DOI QR코드

DOI QR Code

Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

소리쟁이 분획물의 지방세포 분화 억제 효과

  • Received : 2012.08.16
  • Accepted : 2012.11.07
  • Published : 2013.02.28

Abstract

The anti-obesity effect of ethanol xtract and their fractions from Rumex Crispus L. on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibited adipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone- and insulin-along with ethyl acetate fraction residue processing treatment significantly decreased protein expression of obesity-related proteins, such as peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins ${\alpha}$ ($C/EBP{\alpha}$). These results indicate that ethyl acetate fraction of Rumex crispus L. is the most effective candidate for preventing obesity. However further studies will be needed to identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.

Keywords

Rumex Crispus L.;adipocytes;$PPAR{\gamma}$;$C/EBP{\alpha}$;SREBP1c

References

  1. Gregoire FM, Smas CM, Sul HS. Under standing adipocyte differentiation. Physiol. Rev. 78: 783-809 (1998)
  2. Liu F, Kim J, Li Y, Liu X, Li J, Chen X. An extract of Lagerstroemia speciosa L. has insulin-like uptake-stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J. Nutr. 131: 2242-2247 (2001)
  3. Gesta S, Tseng YH, Kahn CR. Developmental origin of fat: Tracking obesity to its source. Cell 131: 242-256 (2007) https://doi.org/10.1016/j.cell.2007.10.004
  4. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Gene. Dev. 14: 1293-1307 (2000)
  5. Ko BS, Lee MY, Kim HK, Chun JM, Choi SB, Jun DW, Jang JS, Park SM. Effect of Cinnamomum camphora leaf fractions on insulin action. J. Korean Soc. Food Sci. Nutr. 34: 1336-1343 (2005) https://doi.org/10.3746/jkfn.2005.34.9.1336
  6. Kim JB, Park JY. Molecular insights into fat cell differentiation and functional roles of adipocytokines. J. Korean Soc. Endo. 17: 1-9 (2002)
  7. Horton JD, Goldstein JL, Brown MS. SREBPs: Transcriptional mediators of lipid homeostasis. Cold Spring Harb. Sym. 67: 491- 498 (2002) https://doi.org/10.1101/sqb.2002.67.491
  8. Zhi J, Moore R, Kanitra L, Mulligan TE. Effects of orlistat, a lipase inhibitor, on the pharmacokinetics of three highly lipophilic drugs (amiodarone, fluoxetine, and simvastatin) in healthy volunteers. J. Clin. Pharmacol. 43: 428-435 (2003) https://doi.org/10.1177/0091270003252236
  9. Ahmad FA, Mahmud S. Acute pancreatitis following orlistat therapy: Report of two cases. J. Pancreas 11: c61-c63 (2010)
  10. Karamadoukis L, Shivashankar GH, Ludeman L, Williams AJ. An unusual complication of treatment with orlistat. Clin. Nephrol. 71: 430-432 (2009) https://doi.org/10.5414/CNP71430
  11. Jeong GT, Lee KM, Park DH. Study of antimicrobial and antioxidant activities of Rumex crispus extract. Korean Chem. Eng. Res. 44: 81-86 (2006)
  12. Chang SW, Kim IH, Han TJ. Antraquinone productivities by the cultures of adventitious roots and hairy roots from culed dock (Rumex crispus). Korean J. Plant Tissue Culture 26: 7-14 (1999)
  13. Hwang SW, Ha TJ, Lee JR, Nam SH, Park KH, Yang MS. Isolation of antraquinone derivatives from the root of Rumex crispus L. J. Korean Soc. Appl. Biol. Chem. 47: 274-278 (2004)
  14. Lee SS, Kim DH, Yim DS, Lee SK. Antiinflammatory, analgesic, and hepatoprotective effect of semen of Rumex crispus. Korean J. Pharmacogn. 38: 334-338 (2007)
  15. Green LM, Reade JL, Ware CF. Rapid colorimetric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines. J. Immunol. Methodsi. 70: 257-268 (1984) https://doi.org/10.1016/0022-1759(84)90190-X
  16. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat. Rev. Mol. Cell Bio. 7: 885-896 (2006) https://doi.org/10.1038/nrm2066
  17. Flier JS, Maratos-Flier E. Obesity and the hypothalamus: novel peptides for new pathways. Cell 92: 437-440 (1998) https://doi.org/10.1016/S0092-8674(00)80937-X
  18. Steel RGD, Torrie JH. Principles and Procedures of Satistics. McGraw Hill, New York, USA. pp.000-000 (1990)
  19. Smith PD, O'Halloran P, Hahn DL, Grasmick M, Radant L. Screening forobesity: Clinical tools in evolution, a WREN study. Wisc. Med. J. 109: 274-278 (2010)
  20. Pi-Sunyer X. The medical risks of obesity. Postgrad. Med. 121: 21-33 (2009) https://doi.org/10.3810/pgm.2009.11.2074
  21. Despres JP, Lemieux I. Abdominal obesity and metabolic syndrome. Nature 444: 881-887 (2006) https://doi.org/10.1038/nature05488
  22. Hotamisligil GS. Inflammation and metabolic disorders. Nature 444: 860-867 (2006) https://doi.org/10.1038/nature05485
  23. Attie AD, Scherer PE. Adipocyte metabolism and obesity. J. Lipid Res. 50: 395-399 (2009)
  24. Cornelius P, MacDougald OA, Lane MD: Regulation of adipocyte development. Annu. Rev. Nutr. 14: 99-129 (1994) https://doi.org/10.1146/annurev.nu.14.070194.000531
  25. Hauser S, Adelmant G, Sarraf P, Wright HM, Mueller E, Spiegelman BM. Degradation of the peroxisome proliferator-activated receptor gamma is linked to ligand-dependent activation. J. Biol. Chem. 275: 18527-18533 (2000) https://doi.org/10.1074/jbc.M001297200
  26. Ohshima T, Koga H, Shimotohno K. Transcriptional activity of peroxisome proliferator-activated receptor gamma is modulated by SUMO-1 modification. J. Biol. Chem. 279: 29551-29557 (2004) https://doi.org/10.1074/jbc.M403866200
  27. Darlington GJ, Ross SE, MacDougald OA. The role of C/EBP genes in adipocyte differentiation. J. Biol. Chem. 273: 30057- 30060 (1998) https://doi.org/10.1074/jbc.273.46.30057
  28. Kim HJ, Hwang EY, Im NK, Park SK, Lee IS. Antioxidant activities of Rumex crispus extracts and effects on quality characteristics of seasoned pork. Korean J. Food Sci. Technol. 42: 445-451 (2010)
  29. Rejman J. Kozubek A. Inhibitory effect of natural phenolic lipids upon NAD-dependent dehydrogenases and on triglyceride accumulation in 3T3-L1 cells in culture. J. Agr. Food Chem. 52: 246- 250 (2004) https://doi.org/10.1021/jf034745a
  30. Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawada T, Lim BO, Park DK. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 75: 3195-3203 (2004) https://doi.org/10.1016/j.lfs.2004.06.012

Cited by

  1. Effects of Mugwort Fumigation on the Body Composition and the Autonomic Nervous System vol.14, pp.3, 2016, https://doi.org/10.20402/ajbc.2016.0053
  2. Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes vol.28, pp.5, 2015, https://doi.org/10.7732/kjpr.2015.28.5.582
  3. Anti-obese and Antioxidant activities of Spica prunellae Extract in 3T3-L1 and HepG2 cells vol.18, pp.4, 2014, https://doi.org/10.13050/foodengprog.2014.18.4.413
  4. Enhancement of Anti-Obesity Activities of Aronia melanocarpa Elliot Extracts from Low Temperature Ultrasonification Process vol.24, pp.4, 2016, https://doi.org/10.7783/KJMCS.2016.24.4.309
  5. Antioxidant Activity and Inhibitory Effect of Aster scaber Thunb. Extract on Adipocyte Differentiation in 3T3-L1 Cells vol.45, pp.3, 2013, https://doi.org/10.9721/KJFST.2013.45.3.356
  6. Inhibitory Effects of Lyophilized Dropwort Vinegar Powder on Adipocyte Differentiation and Inflammation vol.24, pp.5, 2014, https://doi.org/10.5352/JLS.2014.24.5.476
  7. Potential of Fisetin as a Nutri-cosmetics Material through Evaluating Anti-oxidant and Anti-adipogenic Activities vol.14, pp.1, 2016, https://doi.org/10.20402/ajbc.2016.0003
  8. Radical Scavenging and Anti-obesity Effects of Various Extracts from Turmeric (Curcuma longa L.) vol.42, pp.12, 2013, https://doi.org/10.3746/jkfn.2013.42.12.1908

Acknowledgement

Supported by : 대구경북과학기술원