Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

소리쟁이 분획물의 지방세포 분화 억제 효과

  • Park, Sung-Jin (Department of Food Science and Technology, Keimyung University) ;
  • Choi, Jun-Hyeok (Department of Food Science and Technology, Keimyung University) ;
  • Jung, Yeon-Seop (Department of Food Science and Technology, Keimyung University) ;
  • Yu, Mi Hee (Department of Food Science and Technology, Keimyung University)
  • 박성진 (계명대학교 식품가공학 전공) ;
  • 최준혁 (계명대학교 식품가공학 전공) ;
  • 정연섭 (계명대학교 식품가공학 전공) ;
  • 유미희 (계명대학교 식품가공학 전공)
  • Received : 2012.08.16
  • Accepted : 2012.11.07
  • Published : 2013.02.28


The anti-obesity effect of ethanol xtract and their fractions from Rumex Crispus L. on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibited adipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone- and insulin-along with ethyl acetate fraction residue processing treatment significantly decreased protein expression of obesity-related proteins, such as peroxisome-proliferators-activated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins ${\alpha}$ ($C/EBP{\alpha}$). These results indicate that ethyl acetate fraction of Rumex crispus L. is the most effective candidate for preventing obesity. However further studies will be needed to identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.


Supported by : 대구경북과학기술원


  1. Gregoire FM, Smas CM, Sul HS. Under standing adipocyte differentiation. Physiol. Rev. 78: 783-809 (1998)
  2. Liu F, Kim J, Li Y, Liu X, Li J, Chen X. An extract of Lagerstroemia speciosa L. has insulin-like uptake-stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J. Nutr. 131: 2242-2247 (2001)
  3. Gesta S, Tseng YH, Kahn CR. Developmental origin of fat: Tracking obesity to its source. Cell 131: 242-256 (2007)
  4. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Gene. Dev. 14: 1293-1307 (2000)
  5. Ko BS, Lee MY, Kim HK, Chun JM, Choi SB, Jun DW, Jang JS, Park SM. Effect of Cinnamomum camphora leaf fractions on insulin action. J. Korean Soc. Food Sci. Nutr. 34: 1336-1343 (2005)
  6. Kim JB, Park JY. Molecular insights into fat cell differentiation and functional roles of adipocytokines. J. Korean Soc. Endo. 17: 1-9 (2002)
  7. Horton JD, Goldstein JL, Brown MS. SREBPs: Transcriptional mediators of lipid homeostasis. Cold Spring Harb. Sym. 67: 491- 498 (2002)
  8. Zhi J, Moore R, Kanitra L, Mulligan TE. Effects of orlistat, a lipase inhibitor, on the pharmacokinetics of three highly lipophilic drugs (amiodarone, fluoxetine, and simvastatin) in healthy volunteers. J. Clin. Pharmacol. 43: 428-435 (2003)
  9. Ahmad FA, Mahmud S. Acute pancreatitis following orlistat therapy: Report of two cases. J. Pancreas 11: c61-c63 (2010)
  10. Karamadoukis L, Shivashankar GH, Ludeman L, Williams AJ. An unusual complication of treatment with orlistat. Clin. Nephrol. 71: 430-432 (2009)
  11. Jeong GT, Lee KM, Park DH. Study of antimicrobial and antioxidant activities of Rumex crispus extract. Korean Chem. Eng. Res. 44: 81-86 (2006)
  12. Chang SW, Kim IH, Han TJ. Antraquinone productivities by the cultures of adventitious roots and hairy roots from culed dock (Rumex crispus). Korean J. Plant Tissue Culture 26: 7-14 (1999)
  13. Hwang SW, Ha TJ, Lee JR, Nam SH, Park KH, Yang MS. Isolation of antraquinone derivatives from the root of Rumex crispus L. J. Korean Soc. Appl. Biol. Chem. 47: 274-278 (2004)
  14. Lee SS, Kim DH, Yim DS, Lee SK. Antiinflammatory, analgesic, and hepatoprotective effect of semen of Rumex crispus. Korean J. Pharmacogn. 38: 334-338 (2007)
  15. Green LM, Reade JL, Ware CF. Rapid colorimetric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines. J. Immunol. Methodsi. 70: 257-268 (1984)
  16. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat. Rev. Mol. Cell Bio. 7: 885-896 (2006)
  17. Flier JS, Maratos-Flier E. Obesity and the hypothalamus: novel peptides for new pathways. Cell 92: 437-440 (1998)
  18. Steel RGD, Torrie JH. Principles and Procedures of Satistics. McGraw Hill, New York, USA. pp.000-000 (1990)
  19. Smith PD, O'Halloran P, Hahn DL, Grasmick M, Radant L. Screening forobesity: Clinical tools in evolution, a WREN study. Wisc. Med. J. 109: 274-278 (2010)
  20. Pi-Sunyer X. The medical risks of obesity. Postgrad. Med. 121: 21-33 (2009)
  21. Despres JP, Lemieux I. Abdominal obesity and metabolic syndrome. Nature 444: 881-887 (2006)
  22. Hotamisligil GS. Inflammation and metabolic disorders. Nature 444: 860-867 (2006)
  23. Attie AD, Scherer PE. Adipocyte metabolism and obesity. J. Lipid Res. 50: 395-399 (2009)
  24. Cornelius P, MacDougald OA, Lane MD: Regulation of adipocyte development. Annu. Rev. Nutr. 14: 99-129 (1994)
  25. Hauser S, Adelmant G, Sarraf P, Wright HM, Mueller E, Spiegelman BM. Degradation of the peroxisome proliferator-activated receptor gamma is linked to ligand-dependent activation. J. Biol. Chem. 275: 18527-18533 (2000)
  26. Ohshima T, Koga H, Shimotohno K. Transcriptional activity of peroxisome proliferator-activated receptor gamma is modulated by SUMO-1 modification. J. Biol. Chem. 279: 29551-29557 (2004)
  27. Darlington GJ, Ross SE, MacDougald OA. The role of C/EBP genes in adipocyte differentiation. J. Biol. Chem. 273: 30057- 30060 (1998)
  28. Kim HJ, Hwang EY, Im NK, Park SK, Lee IS. Antioxidant activities of Rumex crispus extracts and effects on quality characteristics of seasoned pork. Korean J. Food Sci. Technol. 42: 445-451 (2010)
  29. Rejman J. Kozubek A. Inhibitory effect of natural phenolic lipids upon NAD-dependent dehydrogenases and on triglyceride accumulation in 3T3-L1 cells in culture. J. Agr. Food Chem. 52: 246- 250 (2004)
  30. Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawada T, Lim BO, Park DK. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 75: 3195-3203 (2004)

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