Antioxidant and Anti-adipogenic Effects of PineXol®

PineXol®의 항산화 및 지방세포 분화 억제 효과

  • Lee, Young Jun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Han, Ohan Taek (Nutrapham Co., Ltd.) ;
  • Choi, Hyeon-Son (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Boo Yong (Department of Food Science and Biotechnology, CHA University) ;
  • Chung, Hyun-Jung (Department of Food and Nutrition, Inha University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 이영준 (강원대학교 식품생명공학과) ;
  • 한완택 ((주)뉴트라팜) ;
  • 최현선 (차의과학대학교 식품생명공학과) ;
  • 이부용 (차의과학대학교 식품생명공학과) ;
  • 정현정 (인하대학교 식품영양학과) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2012.11.06
  • Accepted : 2012.12.11
  • Published : 2013.02.28


Pine bark extract is made from the bark of Pinus densiflora which naturally contains occurring phytochemicals such as phenolic compounds. PineXol$^{(R)}$ from products of pine bark extract is sold under the brand name. The aim of this study was to evaluate the total phenol, total flavonoids contents and antioxidant activity of the PineXol$^{(R)}$ as well as to assess the lipid accumulation during adipogenesis of 3T3-L1 cells. Our results demonstrate that the total phenolic and flavonoids contents of the PineXol$^{(R)}$ were $717.40{\pm}6.86$ GAE mg/mL and $54.44{\pm}0.01$ RE mg/mL, respectively. The antioxidative activities of the PineXol$^{(R)}$ were significantly increased in a dose dependent manner on DPPH (1,1-Diphenyl-2-picryl hydrazyl) radical scavenging, ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging, FRAP (ferric reducing antioxidant power) activity, reducing power, nitrite radical scavenging activity and ORAC (Oxygen radical absorbance capacity) value. In addition, the PineXol$^{(R)}$ inhibited the adipocyte differentiation of 3T3-L1 preadipocytes. Exposure to 200 ${\mu}g/mL$, PineXol$^{(R)}$ significantly reduced lipid accumulation (~80%) in 3T3-L1 cells compared to control cells.


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