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Biocontrol of Biofilm-forming Bacillus cereus by Using Organic Acid, Ethanol, and Sodium Chloride

생물막 형성 Bacillus cereus에 대한 유기산, 에탄올 및 NaCl의 제어효과

  • Lee, Young-Duck (Department of Food Science and Biotechnology, Gachon University) ;
  • Yoo, Hye-Lim (Department of Food Science and Biotechnology, Gachon University) ;
  • Park, Jong-Hyun (Department of Food Science and Biotechnology, Gachon University)
  • 이영덕 (가천대학교 식품생물공학과) ;
  • 유혜림 (가천대학교 식품생물공학과) ;
  • 박종현 (가천대학교 식품생물공학과)
  • Received : 2012.10.10
  • Accepted : 2012.10.24
  • Published : 2013.02.28

Abstract

Food poisoning by Bacillus cereus is one of the common food-borne diseases and B. cereus is widely distributed in natural and commercial products owing to the strong resistance caused by biofilm or spore. The ethanol, NaCl, and organic acids of acetic acid, citric acid, and lactic acid for biocontrol of biofilm-forming B. cereus on glass wool were investigated. The biofilm on glass wool was observed in many developments after 48 h incubation. As the results of reduction of biofilm-forming B. cereus by sanitizers, reduction levels of each organic acid treatment ranged to 5-6 log CFU/g-glass wool. In case of combination treatments of 20% ethanol, 10% NaCl, and 1% of each organic acid for 1-5 min, the reduction level of biofilm-forming B. cereus was 7-8 log CFU/g-glass wool. Therefore, combination treatments of ethanol, NaCl, and an organic acid might effectively reduce biofilm-forming B. cereus in various food processes and industries.

Keywords

biofilm;Bacillus cereus;ethanol;NaCl;organic acid

Acknowledgement

Supported by : 농촌진흥청

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