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Characterization of Entomopathogenic Fungus from Trialeurodes vaporariorum and Evaluation as Insecticide

온실가루이 병원성 곰팡이의 특성 및 살충제 개발을 위한 평가

  • Yoon, Hwi Gun (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Shin, Tae Young (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Yu, Mi Ra (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Lee, Won Woo (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Ko, Seung Hyun (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Bae, Sung Min (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Choi, Jae Bang (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Woo, Soo Dong (Department of Plant Medicine, College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • 윤휘건 (충북대학교 농업생명환경대학 식물의학과) ;
  • 신태영 (충북대학교 농업생명환경대학 식물의학과) ;
  • 유미라 (충북대학교 농업생명환경대학 식물의학과) ;
  • 이원우 (충북대학교 농업생명환경대학 식물의학과) ;
  • 고승현 (충북대학교 농업생명환경대학 식물의학과) ;
  • 배성민 (충북대학교 농업생명환경대학 식물의학과) ;
  • 최재방 (충북대학교 농업생명환경대학 식물의학과) ;
  • 우수동 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2013.01.29
  • Accepted : 2013.02.20
  • Published : 2013.03.31

Abstract

The greenhouse whitefly, Trialeurodes vaporariorum, is an economically important pest for greenhouse crops because they cause direct damage by feeding on plant nutrients and indirect damage as transmits many virus vectors. It has recently become a serious problem because of the continuous use of insecticide resulting in resistance among greenhouse whitefly population. To overcome these problems, in this study, the biological characteristics and virulence of an entomopathogenic fungus isolated from the cadaver of nymph greenhouse whitefly were investigated. Isolated fungus was identified as Isaria fumosorosea by morphological examinations and genetic identification using sequences of the ITS, ${\beta}$-tubulin, and EF1-${\alpha}$ regions. This fungus was named as I. fumosorosea SDTv and tested for the virulence against nymphs T. vaporariorum and the cold activity, the thermotolerance and the stability of UV-B irradiation on conidia. Mortality rate of greenhouse whitefly showed from 84 to 100% and the virulence increased with increasing conidial concentrations, $1{\times}10^5$ to $10^8$ conidia/ml. Conidia were stable at $35^{\circ}C$, 0.1 $J/cm^2$ of UV irradiation and germinated after 8 days at $4^{\circ}C$. Additionally, the activities of chitinases and proteases produced by I. fumosorosea SDTv were varied according to the medium. In conclusion, I. fumosorosea SDTv which showed high mortality rate against greenhouse whitefly will be used effectively in the integrated pest management programs against the greenhouse whitefly.

Acknowledgement

Supported by : 한국연구재단, 농림수산식품부

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