- Volume 24 Issue 2
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Study on the Effective Focal Volume Change due to Light Intensity Using Fluorescence Correlation Spectroscopy
형광상관분광법을 이용한 광세기에 따른 유효 초점 부피 변화에 대한 연구
- Jeong, Chanbae (Department of Physics, University of Ulsan) ;
- Lee, Jaeran (Department of Physics, University of Ulsan) ;
- Kim, Sok Won (Department of Physics, University of Ulsan)
- Received : 2013.03.14
- Accepted : 2013.04.19
- Published : 2013.04.25
Using fluorescence correlation spectroscopy, we analyzed the change of effective focal volume of a confocal system with light intensity. The fluorescence correlation spectroscopy system was home-built in accordance with the He-Ne laser with a wavelength of 632.8 nm, and two kinds of samples (AlexaFluor657 and Quantum dot655) suitable for the wavelength of the laser beam were used. For each sample, we analyzed and compared the correlation functions obtained while changing the intensity of the light source in a range of 1~50
Supported by : 한국연구재단
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