Anti-nociceptive Activity of Methanol Extracts from Ligularia stenocephala

곤달비 메탄올 추출물의 진통 효과

  • 차동석 (우석대학교 약학대학) ;
  • 전훈 (우석대학교 약학대학)
  • Received : 2013.03.04
  • Accepted : 2013.03.12
  • Published : 2013.03.31

Abstract

Ligularia stenocephala has been widely used as a traditional medicine for the treatment of lots of diseases such as asthma, arthritis, and jaundice. In this study, we evaluated the anti-nocicepitve activities of methanolic extract of L. stenocephala (MLS) using various pain models including thermal nociception as well as chemical nociception methods. MLS showed significant increase in latency time in the tail immersion test and hot-plate test. In addition, the number of acetic acid-induced abdominal constrictions was decreased by MLS. MLS also attenuated paw licking time in the formalin test. The combination test using naloxone revealed that the anti-nociceptive properties of MLS was not associated with opioid receptor activation. The present results demonstrate that MLS may possibly used as valuable analgesic agent for the treatment of nociceptive pain.

Acknowledgement

Supported by : 우석대학교

References

  1. IASP (1979) The need of a taxonomy. Pain 6: 247-252. https://doi.org/10.1016/0304-3959(79)90046-0
  2. Lynn, B., Winlow, W. and Holden, A. V. (1984) The neurobiology of pain: Symposium of the northern neurobiology group.
  3. Kim, H. J., Chung, W. C., Lee, K. M., Paik, C. N., Jung, S. H., Kim, J. D., Jung, S. H. and Kwak, J. W. (2009) Multiple Gastrointestinal Drug Induced Ulcers Associated with Aspirin and Non-steroidal Anti-inflammatory Drugs: A Case Report and Review of the Literature. The Korean Journal of Gastrointestinal Endoscopy 39: 379-383.
  4. Soledad, M. C., Hernando, A., Olga, M. and Daniel, B. C. (2004) Addition of ultralow dose naloxone to postoperative morphine PCA : unchanged analgesia and opioid requirement but decreased incidence of opioid side effects. Pain 107: 41-46
  5. Calixto, J. B, Beirith, A., Ferreira, J., Snatos, A. R., Filho, V. C. and Yunes, R. A. (2000) Naturally occurring antinociceptive substances from plants. phyther Res. 14: 401-418. https://doi.org/10.1002/1099-1573(200009)14:6<401::AID-PTR762>3.0.CO;2-H
  6. Yaoita, Y., Kikuchi, M. and Machida, K. (2012) Terpenoids and related compounds from plants of the family Compositae (Asteraceae). Nat. Prod. Commun. 7: 533-538.
  7. Lee, B. I., Nugroho, A., Bachri, M. S., Choi, J., Lee, K. R., Choi, J. S., Kim, W. B., Lee, K. T., Lee, J. D. and Park, H. J. (2010) Anti-ulcerogenic effect and HPLC analysis of the caffeoylquinic acid-rich extract from Ligularia stenocephala. Biol. Pharm. Bull. 33: 493-497. https://doi.org/10.1248/bpb.33.493
  8. Toyoda, K,, Yaoita, Y. and Kikuchi, M. (2005) Three new dimeric benzofuran derivatives from the roots of Ligularia stenocephala MATSUM. et KOIDZ. Chem. Pharm. Bull. 53: 1555-1558. https://doi.org/10.1248/cpb.53.1555
  9. Yan, F. L., Wang, A. X. and Jia, Z. J. (2005) Three new polymeric isopropenyl benzofurans from Ligularia stenocephala. Pharmazie., 60: 155-159.
  10. Lee, D. K., Kim, J. K., Kim, J. S., Park, K. J., Cha, D. S. and Jeon, H. (2012) Inhibitory Effect of Ligularia Stenocephala on the Cancer Metastasis Natural Product Sciences 18: 89-96
  11. Zimmermann, M. (1983) Ethical guidelines for investigations of experimental pain in conscious animals. Pain 16: 109-110. https://doi.org/10.1016/0304-3959(83)90201-4
  12. Wang, Y. X., Gao, D., Pettus, M., Phillips, C. and Bowersox, S. S. (2000) Interactions of intrathecally administered ziconotide, a selective blocker of neuronal N-type voltagesensitive calcium channels, with morphine on nociception in Kor. J. Pharmacogn. rats. Pain 84: 271-281. https://doi.org/10.1016/S0304-3959(99)00214-6
  13. Franzotti, E. M., Santos, C. V., Rodrigues, H. M., Mourão, R. H., Andrade, M. R. and Antoniolli, A. R. (2000) Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva-branca). Journal of Ethnopharmacology 72: 273-277 https://doi.org/10.1016/S0378-8741(00)00205-1
  14. Santos, A. R. S. and Calixto, J. B. (1997) Further evidence for the involvement of tachykinin receptor subtypes in formalin and capsaicin models of pain in mice. Neuropeptides 31: 381-389 https://doi.org/10.1016/S0143-4179(97)90075-5
  15. Li, S. and Tator, C. H. (2000) Action of locally administered NMDA and AMPA/kainate receptor antagonists in spinal cord injury. Neurological Research 22: 171-180.
  16. Fukawa, K., Kawano, O., Hibi, M., Misaki, N., Ohba, S. and Hatanaka, Y. (1980) A method for evaluating analgesic agents in rats. J Pharmacol Methods 4: 251-259. https://doi.org/10.1016/0160-5402(80)90017-0
  17. Derardt, R., Jougney, S., Delevalcee, F. and Falhout, M. (1980) Release of prostaglandins E and F in an algogenic reaction and its inhibition. European Journal of Pharmacology 51: 17-24.
  18. Bley, K. R., Hunter, J. C., Eglen, R. M. and Smith, J. A. (1998) The role of IP prostanoid receptors in inflammatory pain. Trends Pharmacol. Sci. 19: 141-147. https://doi.org/10.1016/S0165-6147(98)01185-7
  19. Hunskaar, S., Fasmer, O. B. and Hole K. (1985) Formalin test in mice, a useful technique for evaluating mild analgesics. Journal of Neuroscience Methods 14: 69-76. https://doi.org/10.1016/0165-0270(85)90116-5
  20. Amaral, J. F., Silva, M. I. G., Neto, M. R. A., Neto, P. F. T., Moura, B. A., Melo, C. T. V., Araújo , F. L. O., DeSousa, D. P., Vasconcelos, P. F., Vasconcelos, S. M. and Sousa, F. C. F. (2007) Antinociceptive effect of the monoterpene R-(-)- limoneneinmice. Biol. Pharm. Bull. 30: 1217-1220. https://doi.org/10.1248/bpb.30.1217
  21. Vontagu, H., Abbah, J., Nagazal, I. E., Kunle, O. F., Chindo, B. A., Otsapa, P. B. and Gamaniel, KS. (2004) Anti-nociceptive and anti-inflammatory activities of the methanolic extract of Parinari polyandra stem bark in rats and mice. Journal of Ethnopharmacology 90: 115-121. https://doi.org/10.1016/j.jep.2003.09.038
  22. Yalcin, I. and Aksu, F. (2005) Involvement of potassium channels and nitric oxide in tramadol antinociception. Pharmacol Biochem Behav. 80: 69-75. https://doi.org/10.1016/j.pbb.2004.10.020