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Studies of separation and quantitation for selenium species in food

식품중의 셀레늄 화학종의 분리 및 정량연구

  • Received : 2013.03.06
  • Accepted : 2013.06.04
  • Published : 2013.06.25

Abstract

The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.

Keywords

Ion exchange chromatography;RP HPLC;ICP-MS;HPLC-ICP-MS;Se species;seleno amino acids

References

  1. M. Kotrebai, M. Birringer, J. F. Tyson, E. Block and P. C. Uden, Analyst, 125, 71-78 (2000). https://doi.org/10.1039/a906320j
  2. M. Shah, S. S. Kannamkunarath, J. C. A. Wuilloud, R. G. Wuilloud and J. A. Caruso, J. Anal. Atom. Spectrom., 19, 381-387 (2004). https://doi.org/10.1039/b312320k
  3. C. Ip, M. Birringer, E. Block and M. Kotrebai, J. F. Tyson, P. C. Uden and D. J. Lisk, Agric. Food Chem., 48, 2062-2070 (2000). https://doi.org/10.1021/jf000051f
  4. C. Casiot, J. Szpunar, R. Lobinski and M. Potin-Gautier, J. Anal. Atom. Spectrom., 14, 645-652 (1999). https://doi.org/10.1039/a809027k
  5. J. K. Kirby, G. H. Lyons and M. P. Karkkainen, J. Agric. Food Chem., 56(5), 1772-1779 (2008). https://doi.org/10.1021/jf073030v
  6. S. G. Casal, J. Far, K. Bierla, L. Ouerdane and J. Szpunar, Metallomics, 2(8), 535-548 (2010). https://doi.org/10.1039/c0mt00002g
  7. G. A. Zachariadis, D. C. Kapsimali and E. Rosenberg, Curr. Org. Chem., 14(19), 2282-2299 (2010). https://doi.org/10.2174/138527210793351445
  8. S. Cho, M. Kim, Y. Kim, Y. Min and Y. Kim, Anal. Science & Tech., 19(3), 203-210 (2006).
  9. O. Lee, Y. Jung and J. Moon, Korean J. Nutr., 43, 114-122 (2010). https://doi.org/10.4163/kjn.2010.43.2.114
  10. Y. Ham, B. Kim, Y. Kwon, H. Paik and S. Lee, Anal. Science & Tech., 24(3), 159-167 (2011). https://doi.org/10.5806/AST.2011.24.3.159
  11. S. Yoshida, M. Haratake, T. Fuchigami and M. Nakayama, J. Health Sci., 57, 215-224 (2011). https://doi.org/10.1248/jhs.57.215
  12. J. L. Guzma Mar, L. H. Reyes, G. M. M. Rahman and H. M. S. Kingston, J. Agric. Food Chem., 57, 3005-3013 (2009). https://doi.org/10.1021/jf803598k
  13. O. Wada, N. Kurihara and N. Yamazaki, Jpn. J. Nutr. Assess., 10, 199-210 (1993).
  14. J. L. Shisler, T. G. Senkevich, M. J. Berry and B. Moss, Science, 279, 102-105 (1998). https://doi.org/10.1126/science.279.5347.102
  15. J. Virtamo, E. Valkeila, G. Algrhan, S. Punsar, K. K. Huttnen and M. J. Karvonen, Cancer, 60, 145-148 (1987). https://doi.org/10.1002/1097-0142(19870715)60:2<145::AID-CNCR2820600203>3.0.CO;2-U
  16. K. Kose, P. Dogan and Y. Kardas, Biol. Trace Elem. Res., 53, 51-56 (1996). https://doi.org/10.1007/BF02784544
  17. O. A. Levander. Annu. Rev. Nutriti., 7, 227-250 (1987). https://doi.org/10.1146/annurev.nu.07.070187.001303
  18. J. Far, H. Preud'homme and R. Lobinski, Anal. Chim. Acta, 657, 175-190 (2010). https://doi.org/10.1016/j.aca.2009.10.040
  19. M. Klein, L. Ouerdane, M. Bueno and F. Pannier, Metallomics, 3, 513-520 (2011). https://doi.org/10.1039/c0mt00060d
  20. J. M. Navarrete, L. C. Longoria, M. T. Martinez and L. Cabrera, J. Radioanal. Nucl. Chem., 271, 599-601 (2007). https://doi.org/10.1007/s10967-007-0313-z
  21. P. C. Uden, Anal. Bioanal. Chem., 373, 422-431 (2002). https://doi.org/10.1007/s00216-002-1405-9
  22. X. Dauchy, M. Potin-Gautier, A. Astruc and M. Astruc, Fresen. J. Anal. Chem., 348, 792 (1994). https://doi.org/10.1007/BF01780980
  23. H. Cho and Y. Pak, J. Kor. Chem. Soc., 55(3), 472-477 (2011). https://doi.org/10.5012/jkcs.2011.55.3.472
  24. T. D. Grant, M. Montes-Bayon, D. Leduc, M. W. Fricke, N. Terry and J. A. Caruso, J. Chromatogr. A, 1026, 159-166 (2004). https://doi.org/10.1016/j.chroma.2003.11.039
  25. S. Kokarnig, D. Kuehnelt, M. Stiboller, U. Hartleb and K. A. Francesconi, Anal. Bioanal. Chem., 400, 2323- 2327 (2011). https://doi.org/10.1007/s00216-011-4992-5
  26. Q. Chan, S. E. Afton and J. A. Caruso, J. Anal. Atom. Spectrom., 25, 186-192 (2010). https://doi.org/10.1039/b914157j
  27. Q. Chan, S. E. Afton and J. A. Caruso, Metallomics, 2, 147-153 (2010). https://doi.org/10.1039/b916194e
  28. C. B. Hymer and J. A. Caruso, J. Chromatogr. A, 1045, 1-13 (2004). https://doi.org/10.1016/j.chroma.2004.06.016
  29. A. Prange and D. Pofrock, Anal. Bioanal. Chem., 383, 372-389 (2005). https://doi.org/10.1007/s00216-005-3420-0
  30. T. DeSnaele, P. Verrept, L. Moens and R. Dams, Spectrochim. Acta. B, 50, 1409-1421 (1995). https://doi.org/10.1016/0584-8547(95)01351-6
  31. L. H. Reyes, F. M. Sanz, P. H. Espilez, J. M. Marchante- Gayon, J. I. G. Alonso and A. Sanz-Medel, J. Anal. Atom. Spectrom., 19, 1230-1238 (2004). https://doi.org/10.1039/b403662j
  32. Z. Pedrero, D. Elvira, C. Camara and Y. Madrid, Anal. Chim. Acta., 596, 251-256 (2007). https://doi.org/10.1016/j.aca.2007.05.067

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Acknowledgement

Supported by : 한국연구재단