Studies of separation and quantitation for selenium species in food

식품중의 셀레늄 화학종의 분리 및 정량연구

  • Received : 2013.03.06
  • Accepted : 2013.06.04
  • Published : 2013.06.25


The purpose of this research is to separate and quantitate selenium species in some food samples with HPLC-ICP-MS. Cation exchange chromatography showed efficient separation only for inorganic Se species while reversed phase ion pair chromatography showed good separation for both inorganic and organic Se species. $C_8$ column ($Symmetryshield^{TM}\;RP_8$, 3.5 ${\mu}m$, $4.6{\times}150$ mm) was used with optimum condition of 5% methanol mobile phase, 0.05% of nonafluorovaleric acid ion pairing reagent. Five standard Se species of Se(IV), Se(VI), SeCys(selenocystein), SeMet(selenomethionine) and Se-M-C(seleno methyl cystein) were separated successfully under the optimum condition (mobile phase; 5% methanol, ion-pairing reagent; 0.05% nonafluorovaleric acid, flow rate; 0.9 mL $min^{-1}$). To extract Se species, microwave assisted and enzyme-assisted extraction methods were studied. In enzyme-assisted extraction method, protease I for garlic, protease I plus trypsin for pork and mackerel, and protease XIV for tuna showed the best extraction efficiency. With the optimum condition for each sample, it was found that mostly inorganic Se, SeCys and SeMet are present in the sample studied ranging from few ${\mu}g$ $g^{-1}$ to few tens of ${\mu}g$ $g^{-1}$.


Ion exchange chromatography;RP HPLC;ICP-MS;HPLC-ICP-MS;Se species;seleno amino acids


  1. M. Kotrebai, M. Birringer, J. F. Tyson, E. Block and P. C. Uden, Analyst, 125, 71-78 (2000).
  2. M. Shah, S. S. Kannamkunarath, J. C. A. Wuilloud, R. G. Wuilloud and J. A. Caruso, J. Anal. Atom. Spectrom., 19, 381-387 (2004).
  3. C. Ip, M. Birringer, E. Block and M. Kotrebai, J. F. Tyson, P. C. Uden and D. J. Lisk, Agric. Food Chem., 48, 2062-2070 (2000).
  4. C. Casiot, J. Szpunar, R. Lobinski and M. Potin-Gautier, J. Anal. Atom. Spectrom., 14, 645-652 (1999).
  5. J. K. Kirby, G. H. Lyons and M. P. Karkkainen, J. Agric. Food Chem., 56(5), 1772-1779 (2008).
  6. S. G. Casal, J. Far, K. Bierla, L. Ouerdane and J. Szpunar, Metallomics, 2(8), 535-548 (2010).
  7. G. A. Zachariadis, D. C. Kapsimali and E. Rosenberg, Curr. Org. Chem., 14(19), 2282-2299 (2010).
  8. S. Cho, M. Kim, Y. Kim, Y. Min and Y. Kim, Anal. Science & Tech., 19(3), 203-210 (2006).
  9. O. Lee, Y. Jung and J. Moon, Korean J. Nutr., 43, 114-122 (2010).
  10. Y. Ham, B. Kim, Y. Kwon, H. Paik and S. Lee, Anal. Science & Tech., 24(3), 159-167 (2011).
  11. S. Yoshida, M. Haratake, T. Fuchigami and M. Nakayama, J. Health Sci., 57, 215-224 (2011).
  12. J. L. Guzma Mar, L. H. Reyes, G. M. M. Rahman and H. M. S. Kingston, J. Agric. Food Chem., 57, 3005-3013 (2009).
  13. O. Wada, N. Kurihara and N. Yamazaki, Jpn. J. Nutr. Assess., 10, 199-210 (1993).
  14. J. L. Shisler, T. G. Senkevich, M. J. Berry and B. Moss, Science, 279, 102-105 (1998).
  15. J. Virtamo, E. Valkeila, G. Algrhan, S. Punsar, K. K. Huttnen and M. J. Karvonen, Cancer, 60, 145-148 (1987).<145::AID-CNCR2820600203>3.0.CO;2-U
  16. K. Kose, P. Dogan and Y. Kardas, Biol. Trace Elem. Res., 53, 51-56 (1996).
  17. O. A. Levander. Annu. Rev. Nutriti., 7, 227-250 (1987).
  18. J. Far, H. Preud'homme and R. Lobinski, Anal. Chim. Acta, 657, 175-190 (2010).
  19. M. Klein, L. Ouerdane, M. Bueno and F. Pannier, Metallomics, 3, 513-520 (2011).
  20. J. M. Navarrete, L. C. Longoria, M. T. Martinez and L. Cabrera, J. Radioanal. Nucl. Chem., 271, 599-601 (2007).
  21. P. C. Uden, Anal. Bioanal. Chem., 373, 422-431 (2002).
  22. X. Dauchy, M. Potin-Gautier, A. Astruc and M. Astruc, Fresen. J. Anal. Chem., 348, 792 (1994).
  23. H. Cho and Y. Pak, J. Kor. Chem. Soc., 55(3), 472-477 (2011).
  24. T. D. Grant, M. Montes-Bayon, D. Leduc, M. W. Fricke, N. Terry and J. A. Caruso, J. Chromatogr. A, 1026, 159-166 (2004).
  25. S. Kokarnig, D. Kuehnelt, M. Stiboller, U. Hartleb and K. A. Francesconi, Anal. Bioanal. Chem., 400, 2323- 2327 (2011).
  26. Q. Chan, S. E. Afton and J. A. Caruso, J. Anal. Atom. Spectrom., 25, 186-192 (2010).
  27. Q. Chan, S. E. Afton and J. A. Caruso, Metallomics, 2, 147-153 (2010).
  28. C. B. Hymer and J. A. Caruso, J. Chromatogr. A, 1045, 1-13 (2004).
  29. A. Prange and D. Pofrock, Anal. Bioanal. Chem., 383, 372-389 (2005).
  30. T. DeSnaele, P. Verrept, L. Moens and R. Dams, Spectrochim. Acta. B, 50, 1409-1421 (1995).
  31. L. H. Reyes, F. M. Sanz, P. H. Espilez, J. M. Marchante- Gayon, J. I. G. Alonso and A. Sanz-Medel, J. Anal. Atom. Spectrom., 19, 1230-1238 (2004).
  32. Z. Pedrero, D. Elvira, C. Camara and Y. Madrid, Anal. Chim. Acta., 596, 251-256 (2007).

Cited by

  1. A study of relationship between stomach cancer and selenoproteins in Korean human blood serum vol.28, pp.6, 2015,
  2. Quantitative analysis of selenium species in sea food using solid phase extraction and HPLC-ICP/MS vol.26, pp.5, 2013,
  3. Study of improving precision and accuracy by using an internal standard in post column isotope dilution method for HPLC-ICP/MS vol.27, pp.3, 2014,
  4. Changes of Nutrient Composition and Retention Rate of Sweet Pepper by Different Types and Blanching Method vol.32, pp.4, 2016,
  5. A short study of uncertainty for post column isotope dilution method in HPLC-ICP/MS vol.27, pp.6, 2014,
  6. Quantification of seleno proteins in Korean blood serum using solid phase extraction and affinity chromatography-inductively coupled plasma/mass spectrometry vol.27, pp.2, 2014,


Supported by : 한국연구재단