Screening of Radio-resistant Lactic Acid Bacteria

  • Hwang, E-Nam (Department of Microbial Engineering, Konkuk University) ;
  • Kang, Sang-Mo (Department of Microbial Engineering, Konkuk University) ;
  • Kim, Jae-Kyung (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Jong-Heum (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • Received : 2013.01.11
  • Accepted : 2013.06.03
  • Published : 2013.06.30


This study screened for radio-resistant strains lactic acid bacteria (LAB) by evaluating their capability to survive exposure to ionizing radiation. Ten strains of LAB - Lactobacillus bulgaricus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbruekii, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium breve, and Pediocuccos pentosaceus - were selected and subcultuted twice. The LAB was then further cultured for 3 d at $37^{\circ}C$ to reach 7-10 Log colony-forming units (CFU)/mL prior to irradiation and immediately exposed to gamma rays or electron beams with absorbed doses of 0, 1, 2, 3, 4, 5, 6, 8, and 10 kGy. Gamma irradiation gradually decreased the number of the tested viable LAB, and the effect was irradiation dose dependent. A similar effect was found in electron beam-irradiated LAB. Radiation sensitivity of LAB was calculated as $D_{10}$ values, which ranged from 0.26 kGy to 0.9 kGy and 0.5 kGy to 1.44 kGy with exposure to gamma and electron beam irradiation, respectively, in all tested LAB. L. acidophilus was the most resistant to gamma and electron beam irradiation, with $D_{10}$ values of 0.9 kGy and 1.44 kGy, respectively. These results suggest that L. acidophilus might be suitable for the preparation of probiotics as direct-fed microbes for astronauts in extreme space environments.



  1. Arendt, E. K., Moroni, A., and Zannini, E. (2011) Medical nutrition therapy: use of sourdough lactic acid bacteria as a cell factory for delivering functional biomolecules and food ingredients in gluten free bread. Microb. Cell Fact. 10(suppl 1), S15.
  2. Aween, M. M., Hassan, Z., Muhialdin, B. J., Eljamel, Y. A., Al-Mabrok, A. S., and Lani, M. N. (2012) Antibacterial activity of Lactobacillus acidophilus strains isolated from honey maketed in Malaysia against selected multiple antibiotic resistant (MAR) gram-positive bacteria. J. Food Sci. 77, M364- M367.
  3. Aziz, N. H., El-Fouly, M. Z., Abu-Shady, M. R., and Moussa, L. A. A. (1997) Effect of gamma radiation on the survival of fungal and actinomycetal florae contaminating medicinal plants. Appl. Radiat. Isot. 48, 71-76.
  4. Bari, M. L., Nazuka, E., Sabina, Y., Todoriki, S., and Isshiki, K. (2003) Chemical and irradiation treatments for killing Escherichia coli O157:H7 on alfalfa, radish, and mung bean seeds. J. Food Prot. 66, 767-774.
  5. Byun, M. W. (2009) Current status and prospects of radiation technology for food safety and food security. Bangsaseon San Eob Haghoeji 3, 145-160.
  6. Chen, C. C., Lin, W. C., Kong, M. S., Shi, H. N., Walker, W. A., Lin, C. Y., Huang, C. T., Lin, Y. C., Jung, S. M., and Lin, T. Y. (2012) Oral inoculation of probiotics Lactobacillus acidophilus NCFM suppresses tumor growth both in segmental orthotopic colon cancer and extra-intestinal tissue. Br. J. Nutr. 107, 1623-1634.
  7. Clark, G., Cryan, J. F., Dinan, T. G., and Quigley, E. M. (2012) Probiotics for the treatment of irritable bowel syndrome- Focus on lactic acid bacteria. Aliment Pharnacol. Ther. 35, 403-413.
  8. Hastings, J. W., Holzapfel, W. H., and Noemand, J. G. (1986) Radiation resistance of Lactobacilli isolated from radurized meat relative to growth and environment. Appl. Environ. Microbiol. 52, 898-901.
  9. Horneck, G., Sshafer, M., Baltschukat, K., Weisbrod, U., Micke, U., Facius, R., and Bucker, H. (1989). Cell inactivation, repair, and mutation induction in bacteria after heavy ion exposure: Results from experiments at accelerations and in space. Adv. Space Res. 9, 105-116.
  10. Huang, Y. and Zheng, Y. (2010) The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the downregulation of Niemann-Pick C1-like 1 in Caco-2 cells. Br. J. Nutr. 103, 473-478.
  11. Ilyin, V. K. (2005) Microbiological status of cosmonauts during orbital spaceflight on Salyut and Mir orbital stations. Acta Astonaut. 56, 839-850.
  12. Isolauri, E., Salminen, S., and Ouwehand, A. C. (2004) Probiotics. Best Prac. Res.Cl. Em. 18, 299-313.
  13. Jiang, Y., Lu, X., Man, C., Han, L., Shan, Y., Qu, X., Liu, Y., Yang, S., Xue, Y., and Zhang, Y. (2012) Lactobacillus acidophilus induces cytokine and chemokine production via NFkB and p38 mitogen-activated protein kinase signalling pathway in interstinal epithelial cells. Clin. Vaccine Immunol. 19, 603-608.
  14. Korea Food and Drug Administration. Amendment of health supplemen foods 2008-12. Available from: http://www.mfds. =건강기능식품&division=&pageNo=5.pdf. Accessed Apr. 23, 2013.
  15. Kumar, M., Nagpal, R., Kumar, R., Hemalatha, R., Verma, V., Kumar, A., Chakraborty, C., Sin, B., Marotta, F., Jain, S., and Yadav, H. (2012) Cholesterol-lowering probiotics as potential biotherapeutics for metabolic diseases. Exp. Diabetes Res. 12, 1-14.
  16. Leys, N. M., Hendrickx, L., De Boever, P., Baatout, S., and Mergeay, M. (2004) Space flight effects on bacterial physiology. J. Biol. Regul. Homeost. Agent 18, 193-199.
  17. Maroof, H., Hassan, Z. M., Mobarwz, A. M., and Mohamadabadi, M. A. (2012) Lactobacillus acidophilus could modulate the immune response against breast cancer in murine model. J. Clin. Immunol. 32,1353-1359.
  18. Mercenier, A., Pavan, S., and Pot, B. (2002) Probiotics as biotheraeutic agents: Present knowledge and future prospects. Curr. Pharma. Design 8, 99-110.
  19. Millette, M., Luquet, F. M., and Lacroix, M. (2007) In vitro growth control of selected pathogens by Lactobacillus acidophilus- and Lactobacillus casei-fermented milk. Lett. Appl. Microbiol. 44, 314-319.
  20. Molins, R. A. (2001) Food irradiation: Principles and applications. John Wiley & Sons Inc., New York. 131-191.
  21. Morichi, T. (1997) Characteristic and utilization of lactic acid bacteria; progress in recent researches. Milk Sci. 46, 1-20.
  22. Parvez, S., Malik, K. A., Kang, S. A., and Kim, H. Y. (2006) Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100, 1171-1185.
  23. Rajkowski, K. T., Boyd, G. and Thayer, D. W. (2003) Irradiation D values for Escherichia coli O157:H7 and Salmonella sp. on inoculated broccoli seeds and effects of irradiation on broccoli sprout keeping quality and seed viability. J. Food Prot. 66, 760-766.
  24. Sinn, D. H., Song, J. H., Kim, H. J., Lee, J. H., Son, H. J., Chang, D. K., Kim, Y. H., Kim, J. J., Rhee, J. C., and Rhee, P. L. (2008) Therapeutic effect of Lactobailuus acidophilus- SDC 2012, 2013 in patients with irritable bowel syndrome. Dig. Dis. Sci. 53, 2714-1718.
  25. Wen, K., Li, G., Liu, F., Li, Y., Kocher, J., Lin, L., Yang, X., and Yuan, L. (2012) High dose and low dose Lactobacillus acidophilus exerted differential immune modulating effects on T cell immune responses induced by an oral human rotavirus vaccine in gnotobiotic pigs. Vaccine 30, 1198-1207.
  26. Yadav, H., Jain, S., and Sinha, P. R. (2007) Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats. Nutrition 23, 62-68.
  27. Yang, Y. J., Chuang, C. C., Yang, H. B., Lu, C. C., and Sheu, B. S. (2012) Lactobacillus acidophilus ameliorates H. pyloriinduced gastric inflammation by inactivating the Smad7 and $NF-{\kappa}B$ pathways. BMC Microbiol. 12, 38.
  28. Youn, K. S. and Jeong, Y. N. Market trend in fermented milk products. Abailable from: view.asp?seq=356257. Accessed May 10, 2010.

Cited by

  1. A study of the effects of therapeutic doses of ionizing radiation in vitro on Lactobacillus isolates originating from the vagina - a pilot study vol.16, pp.1, 2016,
  2. Screening of Immune-Active Lactic Acid Bacteria vol.35, pp.4, 2015,