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Bactericidal effect of 461 nm blue light emitting diode on pathogenic bacteria

461nm 청색 LED를 이용한 식중독세균의 살균효과

  • Do, Jung Sun (LED-IT Fusion Technology Research Center, Yeungnam University) ;
  • Bang, Woo Suk (LED-IT Fusion Technology Research Center, Yeungnam University)
  • 도정선 (영남대학교 LED-IT융합산업화연구센터) ;
  • 방우석 (영남대학교 LED-IT융합산업화연구센터)
  • Received : 2013.05.02
  • Accepted : 2013.06.05
  • Published : 2013.06.30

Abstract

The objective of this study was to characterize the bactericidal effect of 461nm visible-light LED on three common foodborne bacteria: Escherichia coli O157:H7, Staphylococcus aureus and Vibrio parahaemolyticus. Tests were conducted against pathogen strains that were treated with 461nm LED for 10 h at $15^{\circ}C$. The E. coli (ATCC 43894, ATCC 8739 and ATCC 35150) and the S. aureus (ATCC 27664, ATCC 19095 and ATCC 43300) had average reductions of 2.5, 6.6, 1.5, 2.5 and 2.0 log CFU/mL, respectively, after they were exposed for 10 h to 461nm LED light (p<0.05). In contrast, V. parahaemolyticus (ATCC 43969) had 6 log CFU/mL reductions after it was exposed for 4 h to 461nm LED light. The results showed that both the Gram-positive and Gram-negative bacteria were inactivated with 461nm LED light exposure. Also, the Gram-negative bacteria were more sensitive to the LED treatment than the Gram-positive bacteria. These results show the potential use of 461nm LED as a food preservation and application technology.

Acknowledgement

Supported by : 산업통상자원부

References

  1. Korea Food and Drug Administration (2012) Available from: http://www.mfds.go.kr/e-stat/index.do Accessed on 2012
  2. Kim YS, Shin DH (2003) Researches on the volatile antimicrobial compounds from edible plants and their food application. Korean J Food Sci Technol, 35, 159-165
  3. Kim YS, Park IS, Ha SD (2009) Application sanitizer for the control of microorganisms in Food. Food Sci Indus, 42, 26-35
  4. Shin JK, Kim BR, Kim AJ (2010) Nonthermal food processing technology using electric Power. Food Sci Indus, 43, 21-34
  5. Um GY (2008) display engineering. gijeon, Seoul, Korea p 320-368
  6. Nam SY, Park MS, Gang JG (2010) The LED technology and application of green energy. SangHakdang, Seoul, Korea p 11-114
  7. BIR research group (2010) Eco-friendly, high-efficient LED technology development trends and market outlook. BIR Inc, Seoul, Korea p 27-188
  8. United States Environmental Protection Agency (2003) Ultraviolet disinfection guidance manual. NSCEP, EPA 815-D-03-007
  9. Young AR (2006) Acute effects of UVR on human eyes and skin. Prog Biophys Mol Biol, 92, 80-85 https://doi.org/10.1016/j.pbiomolbio.2006.02.005
  10. Halliday GM, Norval M, Byrne SN, Huang XX, Wolf P (2008) The effects of sunlight on the skin. Drug discovery today: Disease Mechanisms, 5, 201-209 https://doi.org/10.1016/j.ddmod.2009.09.001
  11. Ikehata H, Ono T (2011) The mechanisms of UV mutagenesis. J Radiat Res, 52, 115-125 https://doi.org/10.1269/jrr.10175
  12. Maisch T (2007) Anti-microbial photodynamic therapy: useful in the future? Lasers Med Sci, 22, 83-91 https://doi.org/10.1007/s10103-006-0409-7
  13. Endarko E, Maclean M, Timoshkin IV, Macgregor SJ, Anderson JG (2012) High-Intensity 405 nm Light Inactivation of Listeria monocytogenes. J photochem photobiol, 88, 1280-1286 https://doi.org/10.1111/j.1751-1097.2012.01173.x
  14. Luksiene Z (2005) New approach to inactivation of harmful and pathogenic microorganisms by Photosensitization. Food Technol Biotechnol, 43, 411-418
  15. Luksiene Z, Zukauskas A (2009) Prospects of photosensitization in control of pathogenic and harmful micro-organisms. J Appl Microbiol, 107, 1415-1424 https://doi.org/10.1111/j.1365-2672.2009.04341.x
  16. Buchovec I, Paskeviciute E, Luksiene Z (2010) Photosensitization-based inactivation of food pathogen Listeria monocytogenes in vitro and on the surface of packaging material. J Photochem Photobiol B, 99, 9-14 https://doi.org/10.1016/j.jphotobiol.2010.01.007
  17. Barolet D (2008) Light-emitting diodes (LEDs) in dermatology. Semin Cutan Med Surg, 27, 227-238 https://doi.org/10.1016/j.sder.2008.08.003
  18. Baek KH, Jang MH, Kwack YB, Lee SW, Yun HK (2010) Regulation of acid contents in kiwifruit irradiated by various wavelength of light emitting diode during postharvest storage. Clean Tech, 16, 88-94
  19. Oh MS, Lee HS (2010) Development of phototactic test apparatus equipped with light source for monitoring Pests. J Appl Biol Chem, 53, 248-252 https://doi.org/10.3839/jabc.2010.043
  20. Oh SJ, Park DS, Yang HS, Yoon YH, Honjo T (2007) Bioremediation on the benrhic layer in polluted inner bay by promotion of microphytobenthos growth using Light Emitting Diode (LED). J Kor Soc MEE, 10, 93-101
  21. Durantini EN (2006) Photodynamic inactivation of bacteria. Curr Bioactive Compounds, 2, 127-142 https://doi.org/10.2174/157340706777435158
  22. Kim SW (2011) In vitro bactericidal effect of red, green and blue (RGB) light emitting diode (LED) irradiation. MS thesis Chonnam National University Gwangju, Korea
  23. Murdoch LE, Maclean M, Endarko E, MacGregor SJ, Anderson JG (2012) Bactericidal effects of 405 nm light exposure demonstrated by inactivation of Escherichia, Salmonella, Shigella, Listeria, and Mycobacterium species in liquid suspensions and on exposed surfaces. The Scientific World Journal, 137805, 1-8
  24. Maclean M, MacGregor SJ, Andersom JG, and Woolsey G (2009) Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array. Appl Environ Microbiol, 75, 1932-1937 https://doi.org/10.1128/AEM.01892-08
  25. Maclean M, Macgregor SJ, Anderson JG, Woolsey (2008) The role of oxygen in the visible-light inactivation of Staphylococcus aureus. J Photochem Photobiol, 92, 180-184 https://doi.org/10.1016/j.jphotobiol.2008.06.006
  26. Fotinos N, Convert M, Piffaretti JC, Gurny R, Lange N (2008) Effects on gram-negative and gram-positive bacteria mediated by 5-aminolevulinic Acid and 5-aminolevulinic acid derivatives. Antimicrob Agents Chemother, 52, 1366-1373 https://doi.org/10.1128/AAC.01372-07
  27. Nitzan Y, Salmon DM, Shporen E, Malik Z (2004) ALA induced photodynamic effects on Gram positive and negative bacteria. Photochem Photobiol Sci, 3, 430-435 https://doi.org/10.1039/b315633h

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