Effect of Ponciri Fructus Extracts Fermented with Ganoderma lucidum on the Collagen Synthesis and Expression of Matrix Metalloproteinase-1

콜라겐 합성과 MMP-1 발현에 대한 생물전환 지실 추출물의 효과

  • Lee, Gye Won (Department of Pharmaceutics & Biotechnology, College of Medical Engineering, Konyang University) ;
  • Park, Sung Min (R&D Center, CoSeedBioPharm Co.) ;
  • Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University) ;
  • Cho, Young Ho (Department of Pharmaceutics & Biotechnology, College of Medical Engineering, Konyang University)
  • 이계원 (건양대학교 제약생명공학과) ;
  • 박성민 ((주)코씨드바이오팜 기술연구소) ;
  • 유영춘 (건양대학교 의학과) ;
  • 조영호 (건양대학교 제약생명공학과)
  • Received : 2013.02.13
  • Accepted : 2013.04.21
  • Published : 2013.04.27


Ponciri fructus, the unripe fruits of Poncirus trifoliata, are widely used in oriental traditional medicine as a remedy for inflammation, gastritis, emesis, digestive ulcers, allergy, and dysentery. To study the anti-wrinkle effects of Ponciri fructus extract (PFE) containing flavanone glycosides, PFE was fermented with Ganoderma lucidum mycelia and its biological activities were investigated. In Ponciri fructus extracts fermented with G. lucidum (G-PFE), polyphenol content was $1,021.00{\pm}0.50{\mu}g/mL$ and flavonoid content was $589.41{\pm}0.21{\mu}g/mL$. G-PFE was found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radical by a dose dependent manner, respectively. G-PFE showed higher antioxidant activity than that of PFE. In addition, the photoprotective properties of G-PFE was tested in human dermal fibroblasts (HDF) exposed to UVA radiation. G-PFE inhibited the activity of matrix metalloproteinase-1 (MMP-1) and showed a dose dependent decrease in the expression level of MMP-1. G-PFE also increased collagen biosynthesis in HDF. These results demonstrate that G-PFE could be useful as a potential cosmetic ingredient for anti-wrinkle.


Supported by : 중소기업청


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