Effects of Horse oil on the DNCB-induced Contact Hypersensitivity in Balb/c Mice

마유(馬油)가 DNCB로 유발된 알레르기성 접촉성피부염에 미치는 영향

  • Lee, Young Sun (Research Institute of Korea Horse Industry, Sung Duk University) ;
  • Yoon, Ji-Hyun (Research Institute of Korea Horse Industry, Sung Duk University) ;
  • Kim, Bo-Ae (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Park, Chan Ik (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Yoo, Wang Keun (Department of Health Science, Daegu Haany University) ;
  • Cho, Jae We (Department of Dermatology, College of Medicine, Keimyung University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, College of Oriental Medicine, Daegu Haany University)
  • 이영선 (성덕대학교 대한말산업 연구소) ;
  • 윤지현 (성덕대학교 대한말산업 연구소) ;
  • 김보애 (대구한의대학교 화장품약리학과) ;
  • 박찬익 (대구한의대학교 화장품약리학과) ;
  • 유왕근 (대구한의대학교 보건학부) ;
  • 조재위 (계명대학교 의과대학 피부과학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2013.06.24
  • Accepted : 2013.07.10
  • Published : 2013.07.30


Objectives : Horse oil (HO) has been used long time as the folk medicine of many Asian countries such as Korea, Mongol, China, India and Japan. HO has been used for anti-bacterial, anti-inflammatory, and anti-pruritic purposes in skin. However, it is still largely unknown whether HO modulates the skin condition. In this study, we attempted to evaluate the anti-inflammatory effect of HO on the 1 % of 2, 4-dinitro-1-chlorobenzene (DNCB)-induced contact hypersensitivity in Balb/c mice. Methods : To find the anti-inflammatory effect of HO, contact hypersensitivity, a local inflammatory response of skin, was induced on the back of Balb/c mice by sensitization and repeated application by 1% DNCB and HO treated 2 weeks on the 1% of DNCB-treated Balb/c mice. Excised mice skins were stained with hematoxylin and eosin and serum IgE level was measured by mouse IgE ELISA kit. Results : In this study, we found that HO reduced erythema by 1% of DNCB treated Balb/c mice. Also, HO recovered histopathological features such as the thickening of epidermis, hyperkeratosis and the infiltration of inflammatory cells in 1% of DNCB treated Balb/c mice. In addition, HO reduced IgE level on the serum obtained from blood of 1% of DNCB-treated Balb/c mice. Conclusion : Taken together, these results showed that HO could be used as a pharmaceutical material with anti-inflammatory effects by reducing of erythema, IgE level and recovering of histopathological features skin on DNCB-induced contact hypersensitivity in Balb/c mice model.


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