Pathological description and immunohistochemical demonstration of ovine abortion associated with Toxoplasma gondii in Iran

  • Rassouli, Maryam (Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Razmi, Golam Reza (Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Movassaghi, Ahmad Reza (Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Bassami, Mohammad Reza (Department of Clinical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad) ;
  • Sami, Mehrdad (Department of Clinical Science, School of Veterinary Medicine, Ferdowsi University of Mashhad)
  • Received : 2012.06.23
  • Accepted : 2012.11.14
  • Published : 2013.03.31


The obligatory intracellular protozoan parasite Toxoplasma gondii is a major world wide cause of infectious ovine abortion. In some different diagnostic techniques that are being used to detect this pathogen in ovine fetuses, immunohistochemistry (IHC) is a very sensitive and expensive one. Histopathology is not truly a specific and sensitive test for Toxoplasma infection but it can be helpful to choose some suspected tissues for IHC. In this study 9.5% of 200 samples (aborted ovine fetuses internal organs such as brain, liver, heart, lung, kidney, spleen) (4.6~14.4% with 95% CI) were positive in IHC with a very good logical agreement among different diagnostic techniques (${\kappa}=0.73$, 0.8) and with no significant difference among different fetal age groups (p > 0.05).


  1. Buxton D, Gilmour JS, Angus KW, Blewett DA, Miller JK. Perinatal changes in lambs infected with Toxoplasma gondii. Res Vet Sci 1982, 32, 170-176.
  2. Buxton D, Finlayson J. Experimental infection of pregnant sheep with Toxoplasma gondii: pathological and immunological observations on the placenta and foetus. J Comp Pathol 1986, 96, 319-333.
  3. Buxton D, Anderson IE, Longbottom D, Livingstone M, Wattegedera S, Entrican G. Ovine Chlamydial abortion: characterization of the inflammatory immune response in placental tissues. J Comp Pathol 2002, 127, 133-141.
  4. Chianini F, Adams C, Buxton D. Neuropathological changes in ovine fetuse caused by tickborne fever. Vet Rec 2004, 155, 805-806.
  5. Duncanson P, Terry RS, Smith JE, Hide G. High levels of congenital transmission of Toxoplasma gondii in a commercial sheep flock. Int J Parasitol 2001, 31, 1699-1703.
  6. Fleiss JL, Levin B, Paik MC. Statistical Methods for Rates and Proportions. 3rd ed. pp. 598-626, Wiley, Hoboken, 2003.
  7. Gottstein B, Hentrich B, Wyss R, Thür B, Busato A, Stark KDC, Müller N. Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. Int J Parasitol 1998, 28, 679-691.
  8. Gutierrez J, O'Donovan J, Williams E, Proctor A, Brady C, Marques PX, Worrall S, Nally JE, McElroy M, Bassett H, Sammin D, Buxton D, Maley S, Markey BK. Detection and quantification of Toxoplasma gondii in ovine maternal and foetal tissues from experimentally infected pregnant ewes using real-time PCR. Vet Parasitol 2010, 172, 8-15.
  9. Hartley WJ, Jebson JL, McFarlane D. New Zealand type II abortion in ewes. Aust Vet J 1954, 30, 216-218.
  10. Hartley WJ, Marshall SC. Toxoplasmosis as a cause of ovine perinatal mortality. N Z Vet J 1957, 5, 119-124.
  11. Innes EA, Bartley PM, Buxton D, Katzer F. Ovine toxoplasmosis. Parasitology 2009, 136, 1887-1894.
  12. Kim JH, Kang KI, Kang WC, Sohn HJ, Jean YH, Park BK, Kim Y, Kim DY. Porcine abortion outbreak associated with Toxoplasma gondii in Jeju Island, Korea. J Vet Sci 2009, 10, 147-151.
  13. Luna LG. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd ed. pp. 38-39, McGraw-Hill, New York, 1968.
  14. Morley EK, Williams RH, Hughes JM, Terry RS, Duncanson P, Smith JE, Hide G. Significant familial differences in the frequency of abortion and Toxoplasma gondii infection within a flock of Charollais sheep. Parasitology 2005, 131 (Pt 2), 181-185.
  15. Morley EK, Williams RH, Hughes JM, Thomasson D, Terry RS, Duncanson P, Smith JE, Hide G. Evidence that primary infection of Charollais sheep with Toxoplasma gondii may not prevent foetal infection and abortion in subsequent lambings. Parasitology 2008, 135, 169-173.
  16. Ortega-Mora LM, Gottstein B, Conraths FJ, Buxton D. Protozoal Abortion in Farm Ruminants: Guidelines for Diagnosis and Control. 1st ed. pp. 124-164, CABI, Wallingford, 2007.
  17. Rassouli M, Razmi GR, Bassami MR, Movassaghi AR, Azizzadeh M. Study on ovine abortion associated with Toxoplasma gondii in affected herds of Khorasan Razavi Province, Iran based on PCR detection of fetal brains and maternal serology. Parasitology 2011, 138, 691-697.
  18. Soleimani Rad J. Histology. 2nd ed. pp. 2-3, Golban Medical Publication, Tehran, 2009. [In Persian]
  19. Uggla A, Sjoland L, Dubey JP. Immunohistochemical diagnosis of toxoplasmosis in fetuses and fetal membranes of sheep. Am J Vet Res 1987, 48, 348-351.

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