- Volume 53 Issue 1
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Changes of characterization of Salmonella Typhimurium isolate following sequential exposures to porcine neutrophil
Salmonella Typhimurium의 돼지 호중구내 연속노출에 따른 특성변화
- Lee, Hee-Soo (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Kim, Aeran (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Youn, Min (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Lee, Ji-Youn (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Lim, Suk-Kyung (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Kang, Ho-Young (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
- Yoo, Han Sang (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
- Park, Jung-Won (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Wee, Sung-Hwan (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
- Jung, Suk-Chan (Animal, Plant and Fisheries Quarantine and Inspection Agency)
- 이희수 (농림수산검역검사본부) ;
- 김애란 (농림수산검역검사본부) ;
- 윤민 (농림수산검역검사본부) ;
- 이지연 (농림수산검역검사본부) ;
- 임숙경 (농림수산검역검사본부) ;
- 강호영 (부산대학교 자연과학대학) ;
- 유한상 (서울대학교 수의과대학) ;
- 박중원 (농림수산검역검사본부) ;
- 위성환 (농림수산검역검사본부) ;
- 정석찬 (농림수산검역검사본부)
- Received : 2012.04.10
- Accepted : 2012.12.27
- Published : 2013.03.31
To develop a live vaccine candidate using an attenuated strain of Salmonella Typhimurium (ST), biochemical properties, plasmid profile, PFGE patterns and pathogenic analysis of the ST isolate were carried out after sequential passage of the ST isolate in porcine neutrophils. By the passage, the ability of the neutrophil-adapted isolate to utilize d-xylose was lost, while the ability of the strain to ferment trehalose was delayed after 2 or more days of the culture. Also, changes including deletion of the gene fragments were observed in PFGE analysis of the neutrophil-adapted isolates. Two plasmids, 105kb and 50kb, were cured in the strain passaged over 15 times in porcine neutrophils. The 50% of lethal dose (
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