Characterization of multipotent mesenchymal stem cells isolated from adipose tissue and bone marrow in pigs

돼지 지방 조직 및 골수 유래 성체줄기세포의 성상분석과 다능성에 관한 연구

  • Lee, Ah-Young (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Choe, Gyeong-Im (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Nah, Jin-Ju (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • So, ByungJae (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Kyung-Woo (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Chang, Ki-Yoon (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Song, Jae-Young (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Cha, Sang-Ho (Animal, Plant and Fisheries Quarantine and Inspection Agency)
  • Received : 2012.09.14
  • Accepted : 2013.01.03
  • Published : 2013.03.31


Mesenchymal stem cells (MSCs) have ability to differentiate into multi-lineage cells, which confer a great promise for regenerative medicine to the cells. The aim of this study was to establish a method for isolation and characterization of adipose tissue-derived MSC (pAD-MSC) and bone marrow-derived MSC (pBM-MSC) in pigs. Isolated cells from all tissues were positive for CD29, CD44, CD90 and CD105, but negative for hematopoietic stem cell associated markers, CD45. In addition, the cells expressed the transcription factors, such as Oct4, Sox2, and Nanog by RT-PCR. pAD-MSC and pBM-MSC at early passage successfully differentiated into chondrocytes, osteocytes and adipocytes. Collectively, pig AD-MSC and BM-MSC with multipotency were optimized in our study.


Supported by : 농림수산검역검사본부


  1. Babaie Y, Herwig R, Greber B, Brink TC, Wruck W, Groth D, Lehrach H, Burdon T, Adjaye J. Analysis of OCT4-dependent transcriptional networks regulating selfrenewal and pluripotency in human embryonic stem cells. Stem Cells 2007, 25, 500-510.
  2. Bosch P, Pratt SL, Stice SL. Isolation, characterization, gene modification, and nuclear reprogramming of porcine mesenchymal stem cells. Biol Reprod 2006, 74, 46-57.
  3. Carlin R, Davis D, Weiss M, Schultz B, Troyer D. Expression of early transcription factors Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC) matrix cells. Reprod Biol Endocrinol 2006, 4, 8.
  4. Choi MJ, Byun JH, Kang EJ, Rho GJ, Kim UK, Kim JR, Park BW. Isolation of porcine multipotential skin-derived precursor cells and its multilineage differentiation. J Korean Assoc Oral Maxillofac Surg 2008, 34, 588-593.
  5. Czyz J, Wiese C, Rolletschek A, Blyszczuk P, Cross M, Wobus AM. Potential of embryonic and adult stem cells in vitro. Biol Chem 2003, 384, 1391-1409.
  6. da Silva Meirelles L, Chagastelles PC, Nardi NB. Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci 2006, 119 (Pt 11), 2204-2213.
  7. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 1970, 3, 393-403.
  8. Haynesworth SE, Baber MA, Caplan AI. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 1992, 13, 69-80.
  9. Jaenisch R, Young R. Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 2008, 132, 567-582.
  10. Jung JS. Adipose tissue derived mesenchymal stem cells. J Korean Soc Transplant 2008, 22, 183-196.
  11. Kajahn J, Gorjup E, Tiede S, von Briesen H, Paus R, Kruse C, Danner S. Skin-derived human adult stem cells surprisingly share many features with human pancreatic stem cells. Eur J Cell Biol 2008, 87, 39-46.
  12. Lee RH, Kim B, Choi I, Kim H, Choi HS, Suh K, Bae YC, Jung JS. Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem 2004, 14, 311-324.
  13. Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng 1998, 4, 415-428.
  14. Monaco E, Bionaz M, Hollister SJ, Wheeler MB. Strategies for regeneration of the bone using porcine adult adiposederived mesenchymal stem cells. Theriogenology 2011, 75, 1381-1399.
  15. Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or selfrenewal of ES cells. Nat Genet 2000, 24, 372-376.
  16. Ock SA, Jeon BG, Rho GJ. Comparative characterization of porcine mesenchymal stem cells derived from bone marrow extract and skin tissues. Tissue Eng Part C Methods 2010, 16, 1481-1491.
  17. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science 1999, 284, 143-147.
  18. Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm- Grove CJ, Bovenkerk JE, Pell CL, Johnstone BH, Considine RV, March KL. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 2004, 109, 1292-1298.
  19. Rodriguez AM, Elabd C, Amri EZ, Ailhaud G, Dani C. The human adipose tissue is a source of multipotent stem cells. Biochimie 2005, 87, 125-128.
  20. Sen A, Lea-Currie YR, Sujkowska D, Franklin DM, Wilkison WO, Halvorsen YD, Gimble JM. Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous. J Cell Biochem 2001, 81, 312-319.<312::AID-JCB1046>3.0.CO;2-Q
  21. Silva J, Chambers I, Pollard S, Smith A. Nanog promotes transfer of pluripotency after cell fusion. Nature 2006, 441, 997-1001.
  22. Song SH, Kumar BM, Kang EJ, Lee YM, Kim TH, Ock SA, Lee SL, Jeon BG, Rho GJ. Characterization of porcine multipotent stem/stromal cells derived from skin, adipose, and ovarian tissues and their differentiation in vitro into putative oocyte-like cells. Stem Cells Dev 2011, 20, 1359-1370.
  23. Yang SR, Cho SD, Ahn NS, Jung JW, Park JS, Jo EH, Hwang JW, Kim SH, Lee BH, Kang KS, Lee YS. The role of p38 MAP kinase and c-Jun N-terminal protein kinase signaling in the differentiation and apoptosis of immortalized neural stem cells. Mutat Res 2005, 579, 47-57.