Effect of Ursolic Acid on the Development of Mouse Embryonic Stem Cells under Hypoxia

저산소 상태에서 우르솔산이 배아줄기세포 성장에 미치는 효과

  • Han, Gi Yeon (Seoul High School) ;
  • Park, Jae Hong (Maria Biotech Co.) ;
  • Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) ;
  • Lee, Sei-Jung (BK21 PLUS Program for Creative Veterinary Science Research, Seoul National University)
  • 한기연 (서울고등학교) ;
  • 박재홍 ((주)마리아 바이오텍) ;
  • 오건봉 (국립축산과학원) ;
  • 이세중 (서울대학교 BK21플러스 수의창의인력양성사업단)
  • Received : 2013.09.05
  • Accepted : 2013.10.01
  • Published : 2013.10.30


Ursolic acid (UA) a bio-active ingredient found in a variety of fruits and vegetables, and it has potent antioxidant activity. However, the role of UA in mouse embryonic stem (ES) cells is poorly understood. This study investigated the functional role of UA in regulating the development of mouse ES cells under hypoxia. Hypoxia did not exert a significant effect on the undifferentiated state of mouse ES cells. However, it induced reactive oxygen species (ROS) generation and increased the level of lactate dehydrogenase (LDH) production at 48 h of hypoxic exposure. Conversely, oxidative stress induced by hypoxia was significantly inhibited by UA ($30{\mu}M$) pretreatment. Hypoxia significantly decreased cell survival and the level of [$^3H$] thymidine incorporation, both of which recovered following pretreatment of UA. In addition, UA decreased the apoptotic effect of hypoxia by attenuating caspase-3 cleavage or by recovering cellular inhibition of the apoptotic protein (cIAP)-2 and Bcl-2 expression. We further found that UA decreased senescence-associated beta-galactosidase activity. We suggest that UA is a natural antioxidant and one of the functional modulators of hypoxia-induced survival, apoptosis, proliferation, and aging in mouse ES cells.


Supported by : 농촌진흥청


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