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Bioethanol Production from Eucheuma spinosum using Various Yeasts

Eucheuma spinosum으로부터 다양한 효모를 이용한 바이오에탄올 생산

  • Kim, Min-Ji (Department of Biotechnology, Pukyong National University) ;
  • Kim, Jung-Soo (Department of Biotechnology, Pukyong National University) ;
  • Ra, Chae Hun (Department of Biotechnology, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
  • 김민지 (부경대학교 수산과학대학 생물공학과) ;
  • 김정수 (부경대학교 수산과학대학 생물공학과) ;
  • 라채훈 (부경대학교 수산과학대학 생물공학과) ;
  • 김성구 (부경대학교 수산과학대학 생물공학과)
  • Received : 2013.07.05
  • Accepted : 2013.09.23
  • Published : 2013.10.30

Abstract

Ethanol fermentations were performed using separate hydrolysis and fermentation (SHF) processes with monosaccharides from pretreated seaweed, Eucheuma spinosum as the biomass. The pretreatment was carried out with 11% (w/v) seaweed slurry and 150 mM $H_2SO_4$ at $121^{\circ}C$ for 40 min. Enzyme hydrolysis after $H_2SO_4$ pretreatment was performed with Celluclast 1.5 L at $45^{\circ}C$ for 24 h. Five % active charcoal were added to hydrolysate to removed 5-hydroxy methylfurfural. Ethanol fermentation with 11% (w/v) seaweed hydrolysate was performed for 72~96 h using Kluyvermyces marxianus, Pichia stipits, Saccharomyces cervisiae and Candida tropicalis. Ethanol concentration was reached to 18 g/L by K. marxianus, 16 g/L by P. stipitis, 15 g/L by S. cerevisiae and 10 g/L by C. tropicalis, respectively. The ethanol yield from total monosugar was obtained 0.50 and ethanol productivity was obtained 0.38 g/L/h by K. marxianus.

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