DOI QR코드

DOI QR Code

Stimulatory effects of Bordetella bronchiseptica antigen on bone marrow cells and immune memory responses

골수세포에 대한 Bordetella bronchiseptica 항원의 자극 효과 및 면역기억반응

  • Yim, Seol-Hwa (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University) ;
  • Joo, Hong-Gu (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
  • 임설화 (제주대학교 수의과대학 수의약리학실) ;
  • 주홍구 (제주대학교 수의과대학 수의약리학실)
  • Received : 2014.06.26
  • Accepted : 2014.10.13
  • Published : 2014.12.31

Abstract

Bone marrow is a hematological and immunological organ that provides multiple immune cells, including B lymphocytes, and thus plays a critical role in the efficacy of vaccine. We previously demonstrated that Bordetella (B.) bronchiseptica antigen has high immunogenicity in spleen cells, a peripheral immune organ. In this study, we investigated the immunogenicity of B. bronchiseptica antigen in bone marrow cells, a central immune organ. B. bronchiseptica antigen increased the cellular activity of bone marrow cells and significantly enhanced the production of nitric oxide, IL-6, and TNF-${\alpha}$. Bone marrow cells primed with B. bronchiseptica antigen in vivo were harvested and stimulated with the same antigen in vitro. The stimulation of B. bronchiseptica antigen significantly increased the cellular activity and proliferation rate of the primed cells. B. bronchiseptica antigen also greatly induced the production of antigen-specific antibody in the primed cells. Taken together, the present study demonstrated that B. bronchiseptica antigen can stimulate bone marrow cells, a central immune organ, and recall the immune response of the primed bone marrow cells.

Acknowledgement

Supported by : 농림축산식품부

References

  1. Chanter N, Magyar T, Rutter JM. Interactions between Bordetella bronchiseptica and toxigenic Pasteurella multocida in atrophic rhinitis of pigs. Res Vet Sci 1989, 47, 48-53.
  2. Abbas AK, Lichtman AH. Cellular and Molecular Immunology, 5th ed. pp. 10, Saunders Elsevier, Philadelphia, 2005.
  3. Audibert FM, Lise LD. Adjuvants: current status, clinical perspectives and future prospects. Immunol Today 1993, 14, 281-284. https://doi.org/10.1016/0167-5699(93)90046-N
  4. Bernasconi NL, Traggiai E, Lanzavecchia A. Maintenance of serological memory by polyclonal activation of human memory B cells. Science 2002, 298, 2199-2202. https://doi.org/10.1126/science.1076071
  5. Horiguchi Y. Swine atrophic rhinitis caused by Pasteurella multocida toxin and Bordetella dermonecrotic toxin. Curr Top Microbiol Immunol 2012, 361, 113-129.
  6. Jo Y, Joo HG. Immunostimulatory effects of Bordetella bronchiseptica antigen on mouse spleen cells. J Prev Vet Med 2013, 37, 35-38.
  7. Kang ML, Kang SG, Jiang HL, Guo DD, Lee DY, Rayamahji N, Seo YS, Cho CS, Yoo HS. Chitosan microspheres containing Bordetella bronchiseptica antigens as novel vaccine against atrophic rhinitis in pigs. J Microbiol Biotechnol 2008, 18, 1179-1185.
  8. Kindt TJ, Goldsby RA, Osborne BA. Kuby Immunology. 6th ed. pp. 42, W. H. Freeman and Company, 2007.
  9. Lanzavecchia A, Sallusto F. Toll-like receptors and innate immunity in B-cell activation and antibody responses. Curr Opin Immunol 2007, 19, 268-274. https://doi.org/10.1016/j.coi.2007.04.002
  10. MacMicking J, Xie Q, Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol 1997, 15, 323-350. https://doi.org/10.1146/annurev.immunol.15.1.323
  11. Matsuda T, Yamasaki K, Taga T, Hirano T, Kishimoto T. Current concepts of B cell modulation. Int Rev Immunol 1989, 5, 97-109. https://doi.org/10.3109/08830188909061976
  12. Woo SH, Moon SY, Byon YY, Joo HG. Evaluation of the immunogenicity of Bordetella bronchiseptica, a vaccine antigen. Korean J Vet Res 2014, 54, 75-79. https://doi.org/10.14405/kjvr.2014.54.2.75

Cited by

  1. Bordetella bronchiseptica antigen enhances the production of Mycoplasma hyopneumoniae antigen-specific immunoglobulin G in mice vol.18, pp.3, 2017, https://doi.org/10.4142/jvs.2017.18.3.327