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Localization of Klotho in cisplatin induced acute kidney failure

Cisplatin 유도 급성신부전에서 Klotho 단백질의 발현

  • Park, So-Ra (Department of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University) ;
  • Kim, Tae-Won (Department of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University) ;
  • Kim, Young-Jung (Department of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University) ;
  • Kim, Hyun-Tae (Department of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University) ;
  • Ryu, Si-Yun (Department of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University) ;
  • Jung, Ju-Young (Department of Veterinary Medicine and Institute of Veterinary Science, Chungnam National University)
  • Received : 2014.06.14
  • Accepted : 2014.11.05
  • Published : 2014.12.31

Abstract

Klotho deficiency is an early event in acute kidney injury (AKI) that exacerbates acute kidney damage. The present study explored the expression of Klotho and inflammation related factors in cisplatin-induced AKI. Rats (n = 18) were treated with cisplatin intraperitoneal injection (5 mg/kg) or left untreated as controls (n = 6), then sacrificed at 5 (n = 6) and 10 days (n = 6) treatment. Five days after cisplatin injection, the serum kidney enzymes and kidney cell apoptosis were significantly increased. Moreover, the expression of Klotho was decreased when compared to the control group, especially in the cortex and outer medulla regions. In contrast, inflammation related signals including nuclear factor kappa B, tumor necrosis factor-${\alpha}$, and tumor necrosis factor-like weak inducer of apoptosis were enhanced. However, 10 days after cisplatin injection, Klotho expression was enhanced upon both IHC and Western blot analysis, with slightly recovered renal function and decreased apoptosis. Furthermore, inflammation related signals expression was decreased relative to the 5 days group. Overall, this study confirmed the opposite expression patterns between Klotho and inflammation related signals and their localization in cisplatin-induced AKI kidney.

Acknowledgement

Supported by : 충남대학교

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