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Inhibition of Melanogenesis by Cucurbitacin B from Cucumis sativus L.

오이로부터 분리된 cucurbitacin B의 미백 효능 연구

  • Chang, Yun-Hee (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Choo, Jung-Ha (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Lee, So-Young (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Kim, Tae-Yoon (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Jin, Mu-Hyun (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Chang, Min-Youl (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Lee, Sang-Hwa (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Lee, Cheon-Koo (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.) ;
  • Park, Sun-Gyoo (Cosmetic Research & Development Center, LG Household & Healthcare Ltd.)
  • 장윤희 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 추정하 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 이소영 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 김태윤 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 진무현 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 장민열 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 이상화 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 이천구 (LG 생활건강 기술연구원 화장품 연구소) ;
  • 박선규 (LG 생활건강 기술연구원 화장품 연구소)
  • Received : 2014.11.09
  • Accepted : 2014.12.09
  • Published : 2014.12.31

Abstract

To develop an effective skin whitening agent for cosmetics, we isolated cucurbitacin B from Cucumis sativus L. which has been used as traditional skin lighting regimen by the bioactivity-guided fractionation, and investigated the inhibitory effects of cucurbitacin B on melanogenesis. At a non-cytotoxic concentration, cucurbitacin B reduced melanin contents of B16F1 melanoma cells in a dose-dependent manner. Cucurbitacin B did not directly inhibit mushroom tyrosinase activity, but it inhibited intracellular tyrosinase activity in a dose-dependent manner. Its inhibitory mechanism on melanin biosynthesis was further assessed, and we found that cucurbitacin B significantly decreased the protein level of tyrosinase, a major melanogenic enzymes and MITF, a master transcriptional factor of melanogenesis. In addition, cucurbitacin B increased the expression of WW domain-containing oxidoreductase (WWOX) which is known to function as tumor repressor and inhibits $Wnt/{\beta}$-catenin pathway. Collectively, these results suggest that cucuritacin B from C. sativus could be used as an active ingredient for skin whitening.

Keywords

cucurbitacin B;melanogenesis;tyrosinase;mitf;wwox

Acknowledgement

Supported by : 보건복지부

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