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Anti-obesity Effects of Extracts from Young Akebia quinata D. Leaves

어린 으름잎 추출물(Akebia quinata D. Leaves)의 항비만 효과

  • Jeon, Yongseop (Div. of Food and Nutrition, Chonnam National University) ;
  • You, Yanghee (Research Institute for Human Ecology, Chonnam National University) ;
  • Jun, Woojin (Div. of Food and Nutrition, Chonnam National University)
  • 전용섭 (전남대학교 식품영양과학부) ;
  • 유양희 (전남대학교 생활과학연구소) ;
  • 전우진 (전남대학교 식품영양과학부)
  • Received : 2013.12.02
  • Accepted : 2014.01.13
  • Published : 2014.02.28

Abstract

We investigated the in vitro and in vivo anti-obesity effects of extracts from young Akebia quinata D. leaves, including hot water (AQH) and 80% ethanol (AQE) extracts. The inhibitory effects of AQH and AQE on lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Compared to control, lipid accumulation was significantly reduced by 18.3% with the treatment upon AQE at a concentration of $5{\mu}g/mL$. The levels of intracellular triglycerides and free glycerol were also reduced by 52.8% and 9.1% at the same concentration of AQE. The in vivo anti-obesity effect of AQE was evaluated in terms of body and white adipose tissue weights in ICR mice. Experimental groups were divided into the following five groups: normal diet (ND), high fat diet (HFD), high fat diet with 60 mg/kg/day of Orlistat (HFD-RF), high fat diet with 200 mg/kg/day of AQE (HFD-AL), and high fat diet with 600 mg/kg/day of AQE (HFD-AH). Feeding of HFD for eight weeks resulted in significant increases in body weight as well as weight gain compared to the ND group. HFD-AH group showed reduced body weight, weight gain, epididymal white adipose tissue weight, and perirenal white adipose weight as compared to the HFD group. These results indicate that AQE supplementation might have beneficial effects on anti-obesity by inhibiting lipid accumulation.

Acknowledgement

Supported by : 산학협력기업부설연구소

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