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Cytoprotective Effects of Phaeophyta Extracts from the Coast of Jeju Island in HT-22 Mouse Neuronal Cells

제주 연안 갈조류 추출물의 신경세포 보호효과

  • Shin, Dong-Bum (Dept. of Food Science and Nutrition, Jeju National University) ;
  • Han, Eun-Hye (Dept. of Food Science and Nutrition, Jeju National University) ;
  • Park, Sung-Soo (Dept. of Food Science and Nutrition, Jeju National University)
  • 신동범 (제주대학교 식품영양학과) ;
  • 한은혜 (제주대학교 식품영양학과) ;
  • 박성수 (제주대학교 식품영양학과)
  • Received : 2013.10.08
  • Accepted : 2013.10.21
  • Published : 2014.02.28

Abstract

Marine algae have long been recognized as a health and beauty food, based on its anti-tumor, anti-inflammatory and anti-obesity activities. In this study, methanol extracts were prepared from 10 different phaeophyta, after which DPPH radical scavenging and cytoprotective activities of HT-22 cells against ${\beta}$-amyloid protein ($A{\beta}$), which has neurotoxic effects, were investigated. In DPPH experiments, Ecklonia cava and Ishige okamurai showed strong ROS scavenging activities, whereas eight other phaeophyta including Petalonia binghamiae (P. bin) showed weak ROS scavenging activities. To validate the cytoprotective effects of 10 different phaeophyta in $A{\beta}$-induced HT-22 cells, protein expression levels of APP, BACE1, iNOS, phosphorylated ERK1/2, phosphorylated p38 and phosphorylated JNK1/2 were determined along with MTT assay. In the MTT assay, P. bin showed the best effective cytoprotective activity at a concentrations of $25{\mu}g/mL$, whereas Sargassum confusum, Colpomenia sinuosa, Myelophycus simplex, and Sargassum hemiphyllum showed potential. Determination of protein expression levels related to $A{\beta}$-induced neurotoxicity in the five selected phaeophyta showed that P. bin inhibited BACE1 and iNOS expression in $A{\beta}$-induced HT-22 cells. These results indicate that the cytoprotective effects of P. bin are mediated by suppressing the pathways involving $A{\beta}$-induced ERK and p38 activation.

Acknowledgement

Supported by : 제주대학교

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