Effects of Delphinidin in Anthocyanin on MDA-MB-231 Breast Cancer Cells

Anthocyanin의 Delphinidin이 MDA-MB-231 유방암세포에 미치는 영향

  • Jang, Hye-Yeon (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Song-Hee (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • An, In-Jung (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Hae-Nim (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Hye-Ri (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Young-Seok (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Byung-Kwon (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Byeong-Soo (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kim, Sang-Ki (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Cho, Sung-Dae (School of dentistry, Institute of Oral Bioscience, Chonbuk National University) ;
  • Nam, Jeong-Seok (Lee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science) ;
  • Choi, Chang-Sun (School of Food Science and Technology, Chung-Ang University) ;
  • Jung, Ji-Youn (Dept. of Companion and Laboratory Animal Science, Kongju National University)
  • 장혜연 (공주대학교 특수동물학과) ;
  • 이송희 (공주대학교 특수동물학과) ;
  • 안인정 (공주대학교 특수동물학과) ;
  • 이해님 (공주대학교 특수동물학과) ;
  • 김혜리 (공주대학교 특수동물학과) ;
  • 박영석 (공주대학교 특수동물학과) ;
  • 박병권 (공주대학교 특수동물학과) ;
  • 김병수 (공주대학교 특수동물학과) ;
  • 김상기 (공주대학교 특수동물학과) ;
  • 조성대 (전북대학교 구강병리학교실) ;
  • 남정석 (가천의과학대학교 이길여암당뇨연구원) ;
  • 최창순 (중앙대학교 식품공학부) ;
  • 정지윤 (공주대학교 특수동물학과)
  • Received : 2013.08.27
  • Accepted : 2014.01.14
  • Published : 2014.02.28


This research showed that delphinidin, an anthocyanin present in blueberry, induced apoptosis in MDA-MB-231 breast cancer cells as well as mediated anti-cancer effects in vivo. As a result, growth of cancer cells as assessed by MTT assay decreased in a concentration-dependent manner. Chromatin condensation by DAPI staining significantly increased in a concentration-dependent manner, indicating apoptosis. The level of p53-protein increased while those of anti-apoptotic molecules (Bcl-2, p-$GSK3{\beta}$) decreased in the western blot. Tumor size decreased in cells treated with 10 mg/kg of delphinidin compared with the control group in vivo. Cell apoptosis assessed by TUNEL assay significantly increased, and tumor inhibitory effect was confirmed. Therefore, delphinidin can be developed for cancer preventive medicine due to its growth inhibitory effects and induction of apoptosis in human breast cancer cells.


Supported by : 농촌진흥청


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