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Investigation of Antimicrobial Activity and Stability of Orixa japonica Thunb. Leaf Extract

상산나무 잎 추출물의 항균활성 및 안정성 조사

  • Received : 2013.09.30
  • Accepted : 2013.11.26
  • Published : 2014.02.28

Abstract

The antimicrobial activity of Orixa japonica Thunb. leaf extract towards 13 microorganism strains was evaluated. Both methanol (MEex) and 70% ethanol extracts showed antimicrobial activity towards Streptococcus mutans, Bacillus cereus, Staphylococcus aureus, and Pseudomonas aeruginosa. MEex showed a higher antimicrobial activity than the 70% ethanol extract. In addition, the dichloromethane fraction (DCMfr) of the MEex also had an antimicrobial effect against the microorganisms examined. The minimum inhibitory concentrations (MICs) towards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 49.22, 24.61, 49.22, and 49.22 mg/mL, respectively. In contrast, the MICs of the DCMfr tpwards S. mutans, B. cereus, S. aureus, and P. aeruginosa were 3.31, 0.21, 1.7, and 1.7 mg/mL, respectively. The MEex antimicrobial activity was not affected by a 3 h exposures to pH in the range of 3-11 or by temperatures were maintained between $80^{\circ}C-100^{\circ}C$ for 6 h. However, the MEex antimicrobial activity decreased at a heat treatment of $121^{\circ}C$ 1 h.

Keywords

Orixa japonica Thunb.;antimicrobial activity;heat stability;pH stability

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  1. Evaluation of Antioxidant and Antimicrobial Activities of Solvent Extracts from Coriolus versicolor vol.44, pp.12, 2015, https://doi.org/10.3746/jkfn.2015.44.12.1793
  2. Antimicrobial Effects on Food-Borne Pathogens and the Antioxidant Activity of Torreya Nucifera Extract vol.26, pp.4, 2015, https://doi.org/10.7856/kjcls.2015.26.4.697

Acknowledgement

Supported by : 서울과학기술대학교