DOI QR코드

DOI QR Code

The Enzyme Inhibitory Activity of Ethanol Extracts Derived from Germinated Rough Rice (Oryza sativar L.) Treated by High Pressure

발아와 고압처리에 따른 벼(Oryza sativar L.) 추출물의 효소저해활성

  • Kim, Min Young (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Sang Hoon (Department of Food Science and Technology, Chungbuk National University) ;
  • Jang, Gwi Young (Department of Food Science and Technology, Chungbuk National University) ;
  • Park, Hye Jin (Department of Food Science and Technology, Chungbuk National University) ;
  • Li, Meishan (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Shinje (Center for Fungi and Plant Genome Research, FnP Corp.) ;
  • Lee, Youn Ri (Department of Food and Nutrition, Daejeon Health Sciences College) ;
  • Lee, Junsoo (Department of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon Sang (Department of Food Science and Technology, Chungbuk National University)
  • Received : 2013.09.30
  • Accepted : 2013.11.14
  • Published : 2014.02.28

Abstract

We evaluated the enzyme inhibitory activity of germinated rough rice (Oryza sativar L.) treated by high pressure (30MPa) for 24 h (HP24) and 48 h (HP48). In rice germinated for 1 day, the ${\alpha}$-glucosidase inhibitory activity reached its highest level, 68.32%, at HP48. The ${\alpha}$-amylase inhibitory activity increased from 32.66-57.00% at HP0, to 43.67-74.82% at HP48. On the other hand, the inhibitory activity of angiotensin-converting enzyme increased from 27.98% to 49.42% over the course of the second day of HP48. The inhibitory activity of xanthine oxidase peaked of 67.51% at HP48 and subsequently decreased. Lipase inhibitory activity increased from 24.04-47.91% at HP0, to 29.62-64.63% at HP48. These results provide useful information for the use of germinated rough rice as a functional food material and demonstrate that high-pressure treatment during the germination process efficiently increase enzyme inhibitory activity.

Keywords

rough rice;germination;high-pressure treatment;enzyme inhibitory activity

Acknowledgement

Supported by : 농림축산식품부

References

  1. Kwak TS, Yeo JH. Varietal variation of ripening and physiochemical properies in different rice ecotypes. Korean J. Intl. Agri. 16: 130-135 (2004)
  2. Kang MY, Lee YR, Nam SH. Characterization of the germinated rices to examine an application potentials as functional rice processed foods. J. Korean Food Sci. Technol. 35: 696-701 (2003)
  3. Bae SM, Kim JH, Cho CW, Jeong TJ, Yook HS, Byun MW, Lee SC. Effect of ${\gamma}$-irradiation on the antioxidant activity of rice hull, rice bran and barley bran. J. Korean Soc. Food Sci. Nutr. 31: 246-250 (2002) https://doi.org/10.3746/jkfn.2002.31.2.246
  4. Lee YR, Kim JY, Woo KS, Hwang IG, Kim KH, Kim KJ, Kim JH, Jeong HS. Changes in the chemical and functional components of Korean rough rice before and after germination. Food Sci. Biotechnol. 16: 1006-1010 (2007)
  5. Kim HY, Hwang IG, Joung EM, Kim TM, Kim DJ, Park DS, JS Lee, Jeong HS. Antiproliferation effects of germinated-Korean rough rice extract on human cancer cells. J. Korean Soc. Food Sci. Nutr. 39: 325-330 (2010) https://doi.org/10.3746/jkfn.2010.39.3.325
  6. Kwon SM, Kim CM, Kim YH. Biological charateristics of instant rice treated with high hydrostatic pressure. Food Sci. Indus. 40: 31-35 (2007)
  7. Kim HY, Hwang IG, Kim TM, Park DS, Kim JK, Kim DJ, Lee YR, Lee JS, Jeong HS. Changes in chemical composition of rough rice (Oryza sativa L.) according to germination period. J. Korean Soc. Food Sci. Nutr. 40: 1265-1270 (2011) https://doi.org/10.3746/jkfn.2011.40.9.1265
  8. Kim HY, Hwang IG, Kim TM, Park DS, Kim JH, Kim DJ, Lee JS, Jeong HS. Antioxidant and angiotensin converting enzyme I inhibitory activity on different parts of germinated rough rice. J. Korean Soc. Food Sci. Nutr. 40: 775-780 (2011) https://doi.org/10.3746/jkfn.2011.40.6.775
  9. Kim MY, Lee SH, Jang GY, Park HJ, Li MS, Kim SJ, Lee YR, Lee JS, Jeong HS. Enzyme inhibition activities of ethanol extracts from germination rough rice (Oryza sativar L.). J. Korean Soc. Food Sci. Nutr. 42: 917-923 (2013) https://doi.org/10.3746/jkfn.2013.42.6.917
  10. San Martin MF, Barbosa-Canovas GV, Swanson BG. Food processing by high hydrostatic pressure. Crit. Rev. Food Sci. Nutr. 42: 627-645 (2002) https://doi.org/10.1080/20024091054274
  11. Northrop DB. Effects of high pressure on enzymatic activity. Biochim. Biophys. Acta 1595: 71-79 (2002) https://doi.org/10.1016/S0167-4838(01)00335-1
  12. Eisenmenger MJ, Reyes-De-Corcuera JI. High pressure enhancement of enzymes: A review. Enzyme Microb. Tech. 45: 331-347 (2009) https://doi.org/10.1016/j.enzmictec.2009.08.001
  13. Tibbot BK, Skadsen RW. Molecular cloning and characterization of a gibberellin-inducible, putative ${\alpha}$-glucosidase gene from barley. Plant Mol. Biol. 30: 229-241 (1996) https://doi.org/10.1007/BF00020110
  14. Lim CS, Li CY, Kim YM, Lee WY, Rhee HI. The inhibitory effect of Cornus walteri extract against ${\alpha}$-amylase. J. Korean Soc. Appl. Biol. Chem. 48: 103-108 (2005)
  15. Gua J, Jin YS, Han W, Shim TH, Sa JH, Wang MH. Studies for component analysis, antioxidative activity and ${\alpha}$-glucosidase inhibitory activity from Equisetum arvense. J. Korean Soc. Appl. Biol. Chem. 49: 77-81 (2006)
  16. Kwon EK, Kim YE, Lee CH, Kim HY. Screening of nine herbs with biological activities on ACE inhibition, HMG-CoA reductase inhibition, and fibrinolysis. Korean J. Food Sci. Technol. 38: 691-698 (2006)
  17. Stirpe F, Corte ED. The regulation of rat liver xanthine oxidase. J. Biol Chem. 244: 3855-3863 (1969)
  18. Kim JH, Kim HJ, Kim CY, Jung HY, Kim YO, Ju JY, Shin CS. Development of lipase inhibitors from various derivatives of Monascus pigment produced by Monascus fermentation. Food Chem. 101: 357-364 (2007) https://doi.org/10.1016/j.foodchem.2005.11.055
  19. Kim JW, Kim JK, Song IS, Kwon ES, Youn KS. Comparison of antioxidant and physiological properties of jerusalem artichoke leaves with different extraction processes. J. Korean Soc. Food Sci. Nutr. 42: 68-75 (2013) https://doi.org/10.3746/jkfn.2013.42.1.068
  20. Lee HS, Lee HJ, Yu HJ, Ju DW, Kim Y, Kim CJ, Cho YJ, Kim N, Choi SY, Suh HJ. A comparison between high hydrostatic pressure extraction and heat extraction of ginsenosides from ginseng (Panax ginseng CA Meyer). J. Sci. Food Agr. 91: 1466-1473 (2011) https://doi.org/10.1002/jsfa.4334
  21. Jang YS, Jeong JM. Antioxidative effects and digestive enzyme inhibition of grape seed extract (GSE). J. Korean Soc. Food Sci. Nutr. 39: 783-788 (2010) https://doi.org/10.3746/jkfn.2010.39.6.783
  22. Kim DY, Han GD. High hydrostatic pressure treatment combined with enzymes increase the extractability and bioactivity of fermented rice bran. Innov. Food Sci. Emerg. 16: 191-197 (2012) https://doi.org/10.1016/j.ifset.2012.05.014
  23. Xi J, Shen D, Zhao S, Lu B, Li Y, Zhang R. Characterization of polyphenols from green tea leaves using a high hydrostatic pressure extraction. Int. J. Pharm. 382: 139-143 (2009) https://doi.org/10.1016/j.ijpharm.2009.08.023
  24. Noh H, Song KB. Isolation of an angiotensin converting enzyme inhibitor from Oenathe javanica. Agric. Chem. Biotechnol. 44: 98-99 (2001)
  25. Unger T. The role of the renin-angiotensin system in the development of cardiovascular disease. Am. J. Cardiol. 89: 3-9 (2002)
  26. Shin ZI, Ahn CW, Nam HS, Lee HJ, Lee HJ, Moon TH. Fractionation of angiotensin converting enzyme (ACE) inhibitory peptides from soybean paste. Korean J. Food Sci. Technol. 27: 230-234 (1995)
  27. Hatano T, Yasuhara T, Yoshihara R, Ikegami Y, Matsuda M, Yazaki K, Agata I, Nishibe S, Noro T, Yoshizaki M, Okuda T. Inhibitory effects of galloylated flavonoids on xanthine oxidase. Planta Med. 57: 83-84 (1991) https://doi.org/10.1055/s-2006-960028
  28. Shouqin Z, Junjie Z, Changzhen W. Novel high pressure extraction technology. Int. J. Pharm. 278: 471-474 (2004) https://doi.org/10.1016/j.ijpharm.2004.02.029
  29. Park JA, Park C, Han MH, Kim BW, Chung YH, Choi YH. Inhibition of adipocyte differentiation and adipogenesis by aged black garlic extracts in 3T3-L1 preadipocytes. J. Life Sci. 21: 720-728 (2011) https://doi.org/10.5352/JLS.2011.21.5.720
  30. Vahouny GV, Roy T, Gallo LL, Story JA, Kritchevshy D, Cassidy M. Effect of chonic intake on cholesterol absorption and metabolism in the rat. Am. J. Clin. Nutr. 33: 2182-2188 (1980)

Cited by

  1. Process Parameters on Quality Characteristics of Jacopever (Sebastes schlegeli Hilgendorf) under Treatment of Hydrostatic Pressure vol.29, pp.3, 2016, https://doi.org/10.9799/ksfan.2016.29.3.371