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The Effect of Cordycepin on the Production of Pro-inflammatory Cytokines in Mouse Peritoneal Macrophages

코디세핀이 마우스 복강 대식세포에서 전염증성 사이토카인의 생성에 미치는 영향

  • Seo, Min-Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kang, Byoung-Won (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kim, Min-Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Lee, Hye-Hyeon (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Seo, Kwon-Il (Department of Food Nutrition, Sunchon National University) ;
  • Kim, Kwang-Hyuk (Department of Microbiology, Kosin University College of Medicine) ;
  • Jeong, Yong-Kee (Medi-Farm Industrialization Research Center, Dong-A University)
  • 서민정 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 강병원 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 김민정 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 이혜현 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 서권일 (순천대학교 식품영양학과) ;
  • 김광혁 (고신대학교 의과대학 미생물학교실) ;
  • 정영기 (동아대학교 Medi-Farm 산업화 연구사업단)
  • Received : 2013.06.20
  • Accepted : 2013.12.03
  • Published : 2014.02.28

Abstract

The effect of cordycepin purified from Cordyceps militaris on macrophage activation was investigated in peritoneal macrophages isolated from C57BL6 mice. Lipopolysaccharide-induced mouse peritoneal cells showed that cordycepin treatment increased the expression of the inflammatory cytokines interleukin (IL)-$1{\beta}$, IL-12, and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), leading to early inflammation-mediated reactions, the activation of immunological responses, and T lymphocyte activation. T lymphocytes, activated by a greater production of IL-6, resulted in antibody-generating immune reactions, suggesting that cordycepin was effective at inducing immunological responses. Consistent with the increase in the inflammation-mediating factors including nitric oxide (NO) and hydrogen peroxide ($H_2O_2$), the toxic response of macrophages was activated and effectively induced inflammation. These findings demonstrate that cordycepin is involved in reducing cell injury provoked by inflammatory reactions. Therefore, these results suggest that cordycepin treatment of mouse peritoneal cells induces inflammation-mediated immunological responses and immunostimulation.

Keywords

cordycepin;cytokines;macrophage;inflammation

Acknowledgement

Supported by : 농림수산식품부

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