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Anti-mycobacterial Effects of the Extract of Humulus japonicus

환삼덩굴(Humulus japonicus) 추출물의 항결핵 효과

  • Hong, Min-Sun (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Son, Eun-Soon (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Lee, Sung-Joong (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Lee, Sun-Kyoung (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Lee, Ye-Jin (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Song, Sun-Dae (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Cho, Sang-Nae (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Barry, Clifton E. III (Tuberculosis Research Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health) ;
  • Eum, Seok-Yong (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center)
  • 홍민선 (국제결핵연구소 면역병리부) ;
  • 손은순 (국제결핵연구소 면역병리부) ;
  • 이성중 (국제결핵연구소 면역병리부) ;
  • 이선경 (국제결핵연구소 면역병리부) ;
  • 이예진 (국제결핵연구소 면역병리부) ;
  • 송선대 (국제결핵연구소 면역병리부) ;
  • 조상래 (국제결핵연구소 면역병리부) ;
  • 크립톤 베리 (미국국립보건원 알레르기 및 감염병연구소 결핵연구부) ;
  • 엄석용 (국제결핵연구소 면역병리부)
  • Received : 2013.09.13
  • Accepted : 2013.11.20
  • Published : 2014.02.28

Abstract

The present study aimed to evaluate the in vitro antimycobacterial effects of hop plant, Humulus japonicus. Methanol extract of H. japonicus (MeOH extract) showed strong direct bactericidal effects against Mycobacterium tuberculosis in vitro. Furthermore, the MeOH extract significantly inhibited M. tuberculosis growth in human macrophages. When five fractions obtained from MeOH extract were examined using the same methods, the hexane and ethyl acetate fractions showed bactericidal effects against M. tuberculosis in vitro, whereas the butanol and water fractions inhibited M. tuberculosis growth in macrophages. Because H. japonicus extract exhibited antimycobacterial activity against both free M. tuberculosis in culture medium and intracellular M. tuberculosis in human macrophages, this plant might be a good candidate for development of a new anti-tuberculosis drug.

Keywords

Humulus japonicus;tuberculosis;bactericidal effect;macrophage

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