Effects of Substrate to Inoculum Ratio on the Biochemical Methane Potential of Piggery Slaughterhouse Wastes

  • Yoon, Young-Man (Biogas Research Center, Hankyong National University) ;
  • Kim, Seung-Hwan (Biogas Research Center, Hankyong National University) ;
  • Shin, Kook-Sik (Biogas Research Center, Hankyong National University) ;
  • Kim, Chang-Hyun (Department of Animal Life and Environment Science, Hankyong National University)
  • Received : 2013.08.29
  • Accepted : 2013.10.28
  • Published : 2014.04.01


The aim of this study was to assess the effect of substrate to inoculum ratio (S/I ratio) on the biochemical methane potential (BMP) and anaerobic biodegradability ($D_{deg}$) of different piggery slaughterhouse wastes, such as piggery blood, intestine residue, and digestive tract content. These wastes were sampled from a piggery slaughterhouse located in Kimje, South Korea. Cumulative methane production curves for the wastes were obtained from the anaerobic batch fermentation having different S/I ratios of 0.1, 0.5, 1.0, and 1.5. BMP and anaerobic biodegradabilities ($D_{deg}$) of the wastes were calculated from cumulative methane production data for the tested conditions. At the lowest S/I ration of 0.1, BMPs of piggery blood, intestine residue, and digestive tract content were determined to be 0.799, 0.848, and $1.076Nm^3kg^{-1}-VS_{added}$, respectively, which were above the theoretical methane potentials of 0.539, 0.644, and $0.517Nm^3kg^{-1}-VS_{added}$ for blood, intestine residue, and digestive tract content, respectively. However, BMPs obtained from the higher S/I ratios of 0.5, 1.0, and 1.5 were within the theoretical range for all three types of waste and were not significantly different for the different S/I ratios tested. Anaerobic biodegradabilities calculated from BMP data showed a similar tendency. These results imply that, for BMP assay in an anaerobic reactor, the S/I ratio of anaerobic reactor should be above 0.1 and the inoculum should be sufficiently stabilized to avoid further degradation during the assay.


Supported by : Ministry for Food, Agriculture, Forestry and Fisheries


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