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폐 한방슬러지를 이용한 팽이버섯의 배지개발

Medium development of Flammulina velutipes by using herb medicine refuse

  • 서권일 (순천대학교 식품영양학과) ;
  • 김철호 (경남과학기술대학교 제약공학과) ;
  • 서동철 (순천대학교 생물환경학과) ;
  • 이성태 (순천대학교 생물학과) ;
  • 박경욱 (에스엔제이바이오(주)) ;
  • 이창윤 (그린합명회사 그린피스 버섯연구소) ;
  • 이상원 (경남과학기술대학교 제약공학과)
  • Seo, Kwon-Il (Dept. of Food and Nutrition, Sunchon National University) ;
  • Kim, Chul-Ho (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Seo, Dong-Cheol (Dept. of Bio-Environmental Sciences, Sunchon National University) ;
  • Yee, Sung-Tae (Dept. of Biology, Sunchon National University) ;
  • Park, Kyung-Wuk (S&J Bio Co., Ltd.) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Institude of Green Co., Ltd.) ;
  • Lee, Sang-Won (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 투고 : 2014.03.05
  • 심사 : 2014.04.02
  • 발행 : 2014.03.31

초록

폐 한방슬러지의 pH는 5.3으로 주재료인 콘코프의 pH 5.2와 비슷하였으나, 환원당 함량은 4.8 mg/g으로 콘코프의 1.9 mg/g 보다 2.5배 높게 나타났다. 평판배지를 사용하여 폐 한방슬러지의 첨가농도를 검토한 결과 폐 한방슬러지를 첨가하지 않은 대조구보다 5, 15 및 10%(w/w) 첨가 순으로 팽이버섯 균사 성장이 왕성하였다. 콘코프 및 미송톱밥배지에 폐 한방슬러지를 각각 10% 첨가하여 제조한 배지를 시험관에 충진시킨 다음 팽이버섯 액체종균을 접종하여 $25^{\circ}C$에서 6일 동안 배양한 결과 대조구에서는 약 2.2~3.4 cm, 시험구에서는 약 5.8~6.4 cm의 균사성장이 이루어졌다. 그리고 팽이버섯뿐만 아니라 표고버섯 및 녹각버섯(영지버섯 일종)을 접종한 시험구에서도 동일한 결과를 얻었다. 폐 한방슬러지를 10% 첨가한 배지로 현장실험을 행한 결과 팽이버섯의 수확량은 대조구보다 약 5%정도 떨어졌지만 기형버섯 발생율과 2등품 버섯의 생산량은 시험구에서 월등하게 적었다. 팽이버섯의 생산량이 16만병/1일 행하는 재배사를 기준으로 경제성 분석을 행한 결과 당해 년에는 약 5천만원이 절약되고 그 다음해부터는 인건비와 운영비를 제외한 약 1억3천만원의 생산단가 절약이 예상되었다.

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