Mycelial viability and cultivation characteristics of Hypsizygus marmoreus after long-term storage in different conditions

장기 저장 후 느티만가닥버섯(Hypsizygus marmoreus) 균주의 균사 생존력과 재배 특성에 대한 연구

  • Lee, Hyae-Jung (Greenpeace Mushroom Institute) ;
  • Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Jong-Guk (Department of Life Science and Biotechnology, Kyungpook National University) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Institute)
  • Received : 2014.01.14
  • Accepted : 2014.01.22
  • Published : 2014.03.31


Mycelial viability and cultivation characteristics of strains of Hypsizygus marmoreus after long-term storage were investigated. The experimental conditions were the storage at $4^{\circ}C$ using a slant culture technique with or without mineral oil, and in liquid nitrogen tank in the presence of 10% glycerol or 10% glycerol with 5% trehalose. The myceila of four strains of H. marmoreus were thawed at 9, 21, 33, and 45 months after beginning of the storage, and then the growth of the mycelia was measured on a PDA plate with serial transfers to new plate for the recovery. The mycelial growth data after 45 months showed that the mycelia were mostly viable but not fully active particularly when they were stored in liquid nitrogen with 10% glycerol. The growth activity could be fully recovered after second transfer to new PDA plate. Cultivation of mushroom fruiting body using the recovered mycelia also demonstrated that the storage methods employed in this work were applicable for the long-term storage of H. marmoreus.


Supported by : 농림축산식품부


  1. Boesewinkel HJ. 1976. Storage of fungal cultures in water. Trans Br Mycol Soc 66:183-185.
  2. Ellis JJ. 1979. Preserving fungus strains in sterile waters. Mycologia 7:1072-1075.
  3. Hwang SW. 1968. Investigation of ultra-low temperature for fungal cultures. An evaluation of liquid-nitrogen storage for preservation of selected fungal cultures. Mycologia 60:613-621.
  4. Lee CY, Song HS, Ro HS, Woo JR, You YH. Kim JK. 2012. Comparison of Endo-, Exo-cellular enzyme activity for new strains of Hypsizygus marmoreus. Kor J Life Sci 22(6):837-843.
  5. Jong SC. 1978. The biology and cultivation of edible mushroom. In Conservation of the cultures, p119-135, ed. Chang ST, Hayes WA. Academic Press.
  6. Kang KH, Song JH, Kim HN. 2002. The genetic variations of Pleurotus spp. on subculture. Korean J Mycology 30(1):23-30.
  7. Kobayashi T. 1984. Maintaining cultures of Basidiomycetes by mineral oil method I. Bulletin of Forestry and Forestry Products Research Institute. 325:141-147.
  8. Lee DH, Kim CJ, Shin KS. 1998. Preservation of mushroom cultures in sterile distilled water. Korean J Mycology 26(1): 91-96.
  9. Lee PH, Chi JH, Kim YH. Yu SH. 1999. Comparison in productivity of Pleurotus ostreatus sawdust spawn under different storage conditions. Korean J Mycology 27:319-321.
  10. Li ZQ, Chen YY. 1981. An evaluation of mineral oil seal preservation of Basidiomycetes cultures. Acta Microbiogica Sinica 21:45-52.
  11. McGinnis MR, Padhye AA, Ajello L. 1974. Storage of stock cultures of filamentous fungi, yeasts, and some aerobic Actinomycetes in sterile distilled water. Appl Microbiol 28: 218-222.
  12. Nakasone KK, Peterson SW, Jong SC. 2004. Preservation and distribution of fungal cultures. In Biodiversity of fungi - Inventory and monitoring methods. p37-47, ed. Mueller GM, Bills GF, Foster MS. Elsevier Academic Press.
  13. Nash T. 1966. Chemical constitution and Physical properties of compounds able to protect living cells against damage due to freezing and thawing. In Cryobiology, p179-211, ed. Meryman HT. Academic Press.
  14. Ohashi H. 2010. Trends of mushrrom production and marketing. In "Annual report of mushroom 2010" (ed. by Ohashi, H.). p. 18, Plant's world Co. Ltd.
  15. Peng JT, Wu LC. 1972. Variations in the cultivated mushroom, Agaricus bisporus. Mushroom science 8:103-113.
  16. Smith D, Onions AHS. 1983. A comparison of some preserving techniques for fungi. Trans Br Mycol Soc 80:333-337.
  17. Smith D. 1991. Maintenance of filamentous fungi. In Maintenance of microorganisms and cultured cells - A manual of laboratory methods. In Maintenance of filamentous fungi, p133-159, ed. Kirsop BE, Doyle A. Academic Press.

Cited by

  1. Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes vol.42, pp.4, 2014,