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장기 저장 후 느티만가닥버섯(Hypsizygus marmoreus) 균주의 균사 생존력과 재배 특성에 대한 연구

Mycelial viability and cultivation characteristics of Hypsizygus marmoreus after long-term storage in different conditions

  • Lee, Hyae-Jung (Greenpeace Mushroom Institute) ;
  • Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Jong-Guk (Department of Life Science and Biotechnology, Kyungpook National University) ;
  • Lee, Chang-Yun (Greenpeace Mushroom Institute)
  • 투고 : 2014.01.14
  • 심사 : 2014.01.22
  • 발행 : 2014.03.31

초록

본 연구는 다양한 조건에서 장기 보존한 느티만가닥버섯 균주의 균사 생존력과 재배성능 검증을 수행하였다. 균사 생존력은 장기 보존된 균주를 9, 21, 33, 45개월 단위로 해동하여 PDA 평판배지에 접종한 다음 1, 2, 3, 4, 5차 계대배양에 따른 균사 생장길이를 측정하여 검증하였다. 액체질소에 보존한 균사를 해동 직후 실시한 1차 계대배양에서는 동결보호제로써 Glycerol 10%를 단용으로 처리한 실험구에서 균사생장이 저조한 결과를 나타내었으나, 5차 계대배양에서는 버섯 균사의 생장력이 다른 실험구와 동일한 수준으로 회복되어, 저장방법에 따른 균사의 생존력에는 큰 영향이 없는것으로 판단된다. 그러나 느티만가닥버섯을 액체 질소에 장기간 보존하고자 할 때는 동결보호제로 사용되는 Glycerol 10%에 Trehalose 5%를 첨가하여 보존해야 균사의 생존력을 유지하는데 도움이 된다. 장기 저장에 따른 느티만가닥버섯 균주의 재배 성능 시험에서는 $4^{\circ}C$ 사면배지에 보호제로써 mineral oil을 사용하는 것이 사면배지 자체로 보존하는 것에 비해 수확량이 감소하는 결과를 나타내므로 장기 저장시에 mineral oil을 사용하는 것은 지양해야 할 것으로 판단된다.

과제정보

연구 과제 주관 기관 : 농림축산식품부

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피인용 문헌

  1. Effects of Preservation Period at Low Temperature on the Mycelial Growth and the Lignocellulolytic Enzyme Activities of Basidiomycetes vol.42, pp.4, 2014, https://doi.org/10.4489/KJM.2014.42.4.322