Cultivation conditions for mass production of detoxifying bacterium Pseudomonas sp. HC1 of tolaasin produced by Pseudomonas tolaasii

버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 독소(tolaasin) 저해균 Pseudomonas sp. HC1의 대량배양을 위한 최적 배양조건

  • 이찬중 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영미 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 한주연 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 전창성 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 문지원 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 서장선 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 한혜수 (흙살림) ;
  • 차재순 (충북대학교 식물의학과)
  • Received : 2014.03.03
  • Accepted : 2014.04.01
  • Published : 2014.03.31


Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and $20^{\circ}C$, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% $(NH_4)_2HPO_4$, 4mM $FeCl_3$, and 3.0% cysteine.


Supported by : 농촌진흥청


  1. Brodey CL, Rainey PB, Teste M, Johnstone K, 1991. Bacterial blotch disease of the cultivated mushroom is caused by an ion channel forming lipodepsipeptide toxin. Mol Plant- Microbe Interact. 4:407-411.
  2. Cho KH, Kim YK. 2003. Two types of ion channel formation of tolaasin, a Pseudomonas peptide toxin. FEMS Microbiol Lett. 221:221-226.
  3. Cutri SS, Macauley BJ, Roberts WP. 1984. Characteristics of pathogenic non-fluorescent (smooth) and non-pathogenic fluorescent (rough) forms of Pseudomonas tolaasii and Pseudomonas 'gingeri'. J Appl Bacteriol. 57:91-298.
  4. Goor M, Vantomme R, Swings J, Gillis M, Kersters K, de Ley J. 1986. Phenotypic and genotypic diversity of Pseudomonas tolaasii and white line reacting organisms isolated from cultivated mushrooms. J Gen Microbiol. 132:2249-2264.
  5. Nair NG, Fahy PC. 1972, Bacteria antagonistic to Pseudomonas tolaasii and their control of brown blotch of the cultivated mushroom Agaricus bisporus. J Applied Bacteriology. 35:439-442.
  6. Jourdan F, Lazzaroni S, Mendes BL, Lo Cantrore P, de Julio M, Amodeo P, Iacobellis NS, Evidente A, Motta A. 2003. A lefthanded alpha-helix containing both L- and D-amino acids: the solution structure of the antimicrobial lipodepsipeptide tolaasin. Proteins. 52:534-543.
  7. Kim JW, Kim KH and Kang HJ. 1994. Studies on the pathogenic Pseudomonas causing bacterial disease of cultivated mushroom in Korea. 1. On the causal organisms of the rots of Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Kor J Plant Pathol. 10:197-210 (in Korean)
  8. Lee CJ, Yoo YM, Han JY, Jhune CS, Cheong JC, Moon JW, Suh JS, Han HS, Cha JS. 2013. Isolation of the Bacterium Pseudomonas sp. HC1 Effective in Inactivation of Tolaasin Produced by Pseudomonas tolaasii. Kor J Mycology. 41:248-254.
  9. Nair NG, Fahy PC. 1973. Toxin production by Pseudomonas tolaasii Paine. Aust J biol Sci. 26:509-512
  10. Nutkins JC, Mortishire-Smith RJ, Packman LC, Brodey CL, Rainey PB, Johnstone K, Williams DH. 1991. Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii Paine. J Am Chem Soc. 113 : 2621-2627.
  11. Paine SG. 1919. Studies in bacteriosis II. A brown blotch disease of cultivated mushrooms. Ann Appl biol. 5:206-219.
  12. Rainey PB, Brodey CL, Johnstone K. 1992. Biology of Pseudomonas tolaasii, cause of brown blotch disease of cultivated mushroom. Pages 95-118 in: Advances in Plant Pathology, Vol. 8. Andrews JH and Tommerup I. eds. Academic Press, Inc., New York.
  13. Reasoner DJ, Geldreich EE. 1985. A new medium for the enumeration and subculture of bacteria from potable water, Appl Environ Microbiol. 49:1-7.
  14. Wells JM, Sapers GM, Fett WF, Butterfield JE, Jones JB, Bouzar H, Miller FC. 1996. Postharvest discolorization of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. 'reactans', and P. 'gingeri'. Phytopathology. 86:1098-1104.
  15. Stainer RY, Palleroni NJ, Doudoroff M. 1966. The aerobic pseudomonads: a taxonomic study. J General Microbiol. 43: 159-271.
  16. Tolaas AG. 1915. A Bacterial disease of cultivated mushrooms. Phytopathology. 5:51-54
  17. Tsuneda A, Suyama K, Muradami S, Ohira I. 1995. Occurrence of Pseudomonas tolaasii on fruiting bodies of Lentinula edodes formed on Quercus logs. Mycoscience. 36:283-288.
  18. Wong WC, Fletche, JT, Unsworth BA, Preece TF. 1982. A note on ginger blotch, a new bacterial disease of the cultivated mushroom, Agaricus bisporus. J Appl Bacteriol. 52:43-48.
  19. Young JM. 1970. Drippy gill: a bacterial disease of cultivated mushrooms caused by Pseudomonas agarici n. sp. NZJ Agr Res. 13:977-990.

Cited by

  1. Optimum cultivation conditions for mass production of antagonistic bacterium Pseudomonas azotoformans HC5 effective in antagonistic of brown blotch disease caused by Pseudomonas tolaasii vol.13, pp.2, 2015,