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Cultivation conditions for mass production of detoxifying bacterium Pseudomonas sp. HC1 of tolaasin produced by Pseudomonas tolaasii

버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 독소(tolaasin) 저해균 Pseudomonas sp. HC1의 대량배양을 위한 최적 배양조건

  • 이찬중 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영미 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 한주연 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 전창성 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 문지원 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 서장선 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 한혜수 (흙살림) ;
  • 차재순 (충북대학교 식물의학과)
  • Received : 2014.03.03
  • Accepted : 2014.04.01
  • Published : 2014.03.31

Abstract

Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and $20^{\circ}C$, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% $(NH_4)_2HPO_4$, 4mM $FeCl_3$, and 3.0% cysteine.

Acknowledgement

Supported by : 농촌진흥청

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