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Effects of Dietary Supplementation with Garlic Extract on Immune Responses and Diseases Resistance of Olive Flounder, Paralichthys olivaceus

사료 내 마늘 추출액 첨가가 넙치(Paralichthys olivaceus)의 비특이적 면역반응 및 질병저항성에 미치는 영향

  • Kim, Seung Min (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jun, Lyu Jin (Marine and Environmental Research Institute, Jeju National University) ;
  • Yeo, In-Kyu (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeon, You-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lee, Kyeong-Jun (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeong, Hyun Do (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Jeong, Joon Bum (Faculty of Marine Biomedical Science, Jeju National University)
  • 김승민 (제주대학교 해양의생명과학부) ;
  • 전려진 (제주대학교 해양과환경연구소) ;
  • 여인규 (제주대학교 해양의생명과학부) ;
  • 전유진 (제주대학교 해양의생명과학부) ;
  • 이경준 (제주대학교 해양의생명과학부) ;
  • 정현도 (부경대학교 수산생명의학과) ;
  • 정준범 (제주대학교 해양의생명과학부)
  • Received : 2013.10.11
  • Accepted : 2014.03.25
  • Published : 2014.04.30

Abstract

In this study, we investigated the effects of dietary 1% garlic extract on nonspecific immune responses and fish diseases (Viral Hemorrhagic Septicemia Virus, Vibrio anguillarum, Streptococcus iniae and Edwardsiella tarda) resistance in olive flounder Paralichthys olivaceus. Fish were fed a commercial diets supplemented with 1% garlic extract for 4 weeks. After the 4 weeks feeding experiment, the artificial infection was made by V. anguillarum, S. iniae, E. tarda and VHSV. And the cumulative mortality was monitored for 2 weeks after artificial infection. The cumulative mortalities decreased in all experiments except for group of E. tarda compared to control group. We observed significantly higher levels of the hematocrit, glucose, total protein, lysozyme activity and the macrophage activity in the experimental group compared to the control group. In the experiments of drug sensitivity and MIC using the three bacteria (V. anguillarum, S. iniae and E. tarda), 1% garlic extract was more effective than the previously reported fermented garlic powder. These results suggested that garlic extract can increase the disease resistance of olive flounder against V. anguillarum, S. iniae and VHSV and the ability of nonspecific immune responses.

Acknowledgement

Supported by : 제주대학교

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