Antioxidative Effects of Common and Organic Kale Juices

유기농 및 일반농 케일 착즙액의 항산화 활성

  • Kim, Jong-Dai (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Ok-Hwan (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jong Seok (Dept. of Food Science and Biotechnology, Kangwon National University) ;
  • Park, Kun-Young (Dept. of Food Science and Nutrition, Pusan National University)
  • 김종대 (강원대학교 식품생명공학과) ;
  • 이옥환 (강원대학교 식품생명공학과) ;
  • 이종석 (강원대학교 식품생명공학과) ;
  • 박건영 (부산대학교 식품영양학과)
  • Received : 2013.12.10
  • Accepted : 2014.04.16
  • Published : 2014.05.31


The objective of the present study was to investigate the protective and free radical scavenging effects of conventionally and organically cultivated kale juices against oxidative damage in $LLC-PK_1$ cells. The DPPH, NO, $O_2{^-}$, and ${\cdot}OH$ radical scavenging activities of organically cultivated kale were higher than those of conventionally cultivated kale juice. Oxidative damage induced by AAPH (2,2'-azobis(2-amidinopropane) dihydrochloride), SNP (sodium nitroprusside), pyrogallol, and SIN-1 (3-morpholinosydnonimine) led to loss of cell viability and increased lipid peroxidation in LLC-PK1 cells, whereas treatment with vegetable juices, especially organically cultivated kale juices, significantly increased cell viability and inhibited lipid peroxidation in a dose-dependent manner (P<0.05). These results suggest that organically cultivated kale juices have protective roles against oxidative stress induced by free radicals.


  1. Fridovich I. 1989. Superoxide dismutases. An adaptation to a paramagnetic gas. J Biol Chem 264: 7761-7764.
  2. Lee OH, Lee BY, Lee J, Lee HB, Son JY, Park CS, Shetty K, Kim YC. 2009. Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities. Bioresour Technol 100: 6107-6113.
  3. Shulz H. 1994. Regulation of fatty acid oxidation in heart. J Nutr 124: 165-171.
  4. Halliwell B, Murcia MA, Chirico S, Aruoma OI. 1995. Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr 35: 7-20.
  5. Chung SY, Kim SH, Kim HS, Kang JS, Chung HS, Kim GJ, Kim HJ. 1990. Effects of water soluble extract of Ganoderma lucidum, kale juice and sodium dextrothyroxine on hormone and lipid metabolism in hypercholesterolemic rats: 1. concentration of triiodthyronine, thyroxine, blood sugar and lipid composition in serum. J Korean Soc Food Nutr 19: 381-389.
  6. Byers T, Perry G. 1992. Dietary carotenes, vitamin C, and vitamin E as protective antioxidants in human cancers. Annu Rev Nutr 12: 139-159.
  7. Caragay AB. 1992. Cancer preventive foods and ingredients. Food Tech 46: 65-68.
  8. Stahelin HB, Gey KF, Eichholzer M, Ludin E. 1991. ${\beta}$-Carotene and cancer prevention: the Basel Study. Am J Clin Nutr 53: 265S-269S.
  9. Mertens B, Knorr D. 1992. Developments of nonthermal processes for food preservation. Food Technol 46: 124-133.
  10. Lee DU, Park J, Kang J, Yeo IH. 1996. Effect of high hydrostatic pressure on the shelf-life and sensory characteristics of Angelica keiskei juice. Korean J Food Sci Technol 28: 105-108.
  11. Chung SY, Kim HW, Yoon S. 1999. Analysis of antioxidant nutrients in green yellow vegetable juice. Korean J Food Sci Technol 31: 880-886.
  12. Yang HS. 1993. The effect of green juice. Food Hyg 6: 62-67.
  13. Swarup V, Ahluwalia KS, Roy SK, Chatterjee SS. 1979. Kale-a nutritionally rich vegetable. Indian Horticulture 24: 9-10.
  14. Park KY, Lee K, Rhee SH. 1992. Inhibitory effect of green-yellow vegetables on the mutagenicity in Salmonella assay system and on the growth of AZ-521 human gastric cancer cells. J Korean Soc Food Nutr 21: 149-153.
  15. Lee SM, Rhee SH, Park KY. 1993. Antimutagenic effect of soluble dietary fibers from kale and soybean. Environ Mutagen Carcino 13: 26-35.
  16. Jeon EJ, Kim JS, Park YK, Kim TS, Kang MH. 2003. Protective effect of yellow-green vegetables juices on DNA damage in Chinese hamster lung cell using comet assay. Korean J Nutr 36: 24-31.
  17. Okuyama T, Takata M, Takayasu J, Hasegawa T, Tokuda H, Nishino A, Nishino H, Iwashima A. 1991. Anti-tumor-promotion by principles obtained from Angelica keiskei. Planta Med 57: 242-246.
  18. Park YS, Lim DG, Heo BG. 2009. Changes in the fruit quality of organic and low-level agrochemical-grown kiwi-fruit during storage. Korean J Food Preserv 16: 327-332.
  19. Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 13: 8-17.
  20. Rockett K, Awburn MM, Cowden WB, Clark IA. 1991. Killing of Plasmodium falciparum in vitro by nitric oxide derivatives. Infect Immun 59: 3280-3283.
  21. Niwa Y, Miyachi Y. 1986. Antioxidant action of natural health products and Chinese herbs. Inflammation 10: 79-91.
  22. Smirnoff N, Cumbes QJ. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28: 1057-1060.
  23. Nath KA. 1992. Tubulointerstitial changes as a major determinant in the progression of renal damage. Am J Kidney Dis 20: 1-17.
  24. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
  25. Fraga CG, Leibovitz BE, Tappel AL. 1988. Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med 4: 155-161.
  26. Kim HY, Lee KB, Lim HY. 2004. Contents of minerals and vitamins in organic vegetables. Korean J Food Preserv 11: 424-429.

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