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Hypopigmenting Effects of Extracts from Bulbs of Lilium Oriental Hybrid 'Siberia' in Murine B16/F10 Melanoma Cells

백합뿌리 추출물의 멜라닌 생성 억제효과

  • Yoon, Hoon Seok (Cosmetic Science Center, Dept. of Chemistry, Jeju National University) ;
  • Yang, Kyung-Wol (Jeju Love Co., Ltd.) ;
  • Kim, Jung Eun (Cosmetic Science Center, Dept. of Chemistry, Jeju National University) ;
  • Kim, Jeong Mi (Jeju Love Co., Ltd.) ;
  • Lee, Nam Ho (Cosmetic Science Center, Dept. of Chemistry, Jeju National University) ;
  • Hyun, Chang-Gu (Cosmetic Science Center, Dept. of Chemistry, Jeju National University)
  • 윤훈석 (제주대학교 화장품과학연구센터) ;
  • 양경월 ((주)제주사랑) ;
  • 김정은 (제주대학교 화장품과학연구센터) ;
  • 김정미 ((주)제주사랑) ;
  • 이남호 (제주대학교 화장품과학연구센터) ;
  • 현창구 (제주대학교 화장품과학연구센터)
  • Received : 2014.01.22
  • Accepted : 2014.04.01
  • Published : 2014.05.31

Abstract

In order to develop a skin-whitening agent, melanin contents and intracellular tyrosinase activity were determined by western blotting. Ethyl acetate fractions of 80% ethanol extracts from lily (Lilium Oriental Hybrid 'Siberia') bulbs (R-EA) inhibited melanin synthesis in a dose-dependent manner in ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH)-treated B16/F10 murine melanoma cells. Intracellular tyrosinase activity and melanin contents were suppressed by 45% and 74%, respectively, in response to treatment with $100{\mu}g/mL$ of R-EA. Examination of protein expression associated with ${\alpha}$-MSH-induced melanogenesis showed that tyrosinase related protein (TRP)-1 was inhibited more strongly than tyrosinase, and these results were correlated with stronger inhibition of melanin synthesis than intracellular tyrosinase activity. Taken together, R-EA containing p-coumaric acid and resveratrol could be used as a hypopigmentation agent through suppression of sustained extracellular signal-regulated kinase (ERK) activation via melanogenic induction.

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