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Optimization of Ethanol Extraction Conditions for Artemisis capillaris Effective Components Using Response Surface Methodology

반응표면분석법을 이용한 인진쑥 유효성분의 에탄올 추출조건 최적화

  • Kim, Seong-Ho (Dept. of Food Science and Technology, Daegu University)
  • 김성호 (대구대학교 식품공학과)
  • Received : 2014.03.07
  • Accepted : 2014.04.15
  • Published : 2014.05.31

Abstract

This study was conducted to monitor the quality characteristics of Artemisis capillaris ethanolic extract by response surface methodology. The independent variables were extraction temperature ($X_1$; 60, 70, 80, 90, and $100^{\circ}C$), extraction time ($X_2$; 1, 2, 3, 4, and 5 hr), and ethanol concentration ($X_3$; 0, 20, 40, 60, and 80%). Soluble solid content ($Y_1$), chlorogenic acid content ($Y_2$), and coumaric acid content ($Y_3$), etc. were analyzed as the dependent variables. Estimated optimal conditions for soluble solids were an extraction temperature of $87.65^{\circ}C$, extraction time of 3.19 hr, and ethanol concentration of 42.40%. The optimal extraction conditions for chlorogenic acid were $84.30^{\circ}C$, 3.14 hr, and 47.85%, respectively. Further, those for coumaric acid were $83.45^{\circ}C$, 3.40 hr, and 45.39%, respectively. Extraction conditions for effective components of Artemisis capillaris were superimposed by response surface plots on optimization extraction condition of each dependent variable, including soluble solid, chlorogenic acid, and coumaric acid contents. As a result, superimposed extraction conditions were $80{\sim}90^{\circ}C$, 3~4 hr, and 40~50%, respectively. Under these conditions, soluble solid, chlorogenic acid, and coumaric acid contents were 1.09%, 25.66 mg%, and 20.25 mg%, respectively.

References

  1. KIM TJ, Jang JS. 1996. Korean resources plants VI. Seoul National University Publishing Department Corp., Seoul, Korea. p 259.
  2. Lee SG. 2005. The therapeutic effect of Artemisia capillaris extract on hepatic damage induced by carbon tetrachloride in rats. J Vet Clin 22: 206-213.
  3. Kim H, Kim J, Lim M, Yeo S, Jang K, Oh T, Lee G. 2007. Antimicrobial effects of Artemisia capillaris extracts on the pathogenic bacteria in vitro. J Vet Clin 24: 130-136.
  4. Kim JO, Kim YS, Lee JH, Kim MN, Rhee SH, Moon SH, Park KY. 1992. Antimutagenic effect of the major volatile compounds identified from mugwort (Artemisia asictica nakai) leaves. J Korean Soc Food Nutr 21: 308-313.
  5. Ahn BM. 2000. What is In-Jin-Sook? Artemisia capillaries, Artemisia iwayomogi, and Artemisia annua. Korean J Hepatol 6: 548-551.
  6. Lee GD, Kim JS, Bae JO, Yoon HS. 1991. Antioxidative effectiveness of water extract and ether extract in wormwood (Artemisia montana Pampan). J Korean Soc Food Nutr 21: 17-22.
  7. Kim EJ, Lee CK, Choi JW. 1992. The effect of scoparone on the hepatic bromobenzene metabolizing enzyme system in rats. Kor J Pharmacogn 23: 81-88.
  8. Kiso Y, Ogasawara S, Hirota K, Watanabe N, Oshima Y, Konno C, Hikino H. 1984. Antihepatotoxic principles of Artemisia capillaris buds 1. Planta Med 50: 81-85. https://doi.org/10.1055/s-2007-969627
  9. Komiya T, Naruse Y, Oshio H. 1976. Studies on "Inchinko": I. capillarisin, a new choleretic substance (author's transl). Yakugaku Zasshi 96: 841-854. https://doi.org/10.1248/yakushi1947.96.7_841
  10. Cho YH, Chiang MH. 2001. Essential oil composition and antibacterial activity of Artemisia capillaries, Artemisia argyi, and Artemisia princeps. Kor J Intl Agri 13: 313-320.
  11. Waterfield CJ, Turton JA, Scales MD, Timbrell JA. 1993. Investigations into the effects of various hepatotoxic compounds on urinary and liver taurine levels in rats. Arch Toxicol 67: 244-254. https://doi.org/10.1007/BF01974343
  12. Block G, Langseth L. 1994. Antioxidant vitamins and disease prevention. Food Technol 48: 80-84.
  13. Sheu SJ, Chieh CL, Weng WC. 2001. Capillary electrophoretic determination of the constituents of Artemisiae capillaris Herba. J Chromatography A 911: 285-293. https://doi.org/10.1016/S0021-9673(01)00513-1
  14. Wu TS, Tsang ZJ, Wu PL, Liou MJ, Leu YL, Chan YY, Lin FW, Shi LS. 1998. Phenylalkynes from Artemisia capillaris. Phytochemistry 47: 1645-1648. https://doi.org/10.1016/S0031-9422(97)00777-2
  15. Wu TS, Tsang ZJ, Wu PL, Lin FW, Li CY, Teng CM, Lee KH. 2001. New constituents and antiplatelet aggregation and anti-HIV principles of Artemisia capillaris. Bioorg Med Chem 9: 77-83. https://doi.org/10.1016/S0968-0896(00)00225-X
  16. Kimura Y, Okuda H, Okuda T, Halano T, Agata I, Arichi S. 1985. Studies on the activities of tannins and related compounds from medicinal plants and drugs. VII. Effects of extracts of leaves of Artemisia species, and caffeic acid and chlorogenic acid on lipid metabolic injury in rats fed peroxidized oil. Chem Pharm Bull (Tokyo) 33: 2028-2034. https://doi.org/10.1248/cpb.33.2028
  17. Lim SS, Lee JH. 1997. Effect of Artemisia princeps var orientalis and Circium japonicum var ussuriense on serum lipid of hyperlipidemic rat. Korean J Nutr 30: 12-18.
  18. Wanasundara PKJPD, Shahidi F. 1996. Optimization of hexametaphosphate-assisted extraction of flaxseed proteins using response surface methodology. J Food Sci 6: 604-607.
  19. Abell ML, Braselton JP. 1992. The mathematica handbook: [compatible with mathematica version 2.0]. Academic press Inc., Boston, MA, USA. p 15-511.
  20. Kwon JH, Belanger JM, Pare JR. 2003. Optimization of microwave-assisted extraction (MAP) for ginseng components by response surface methodology. J Agric Food Soc 89: 216-222.
  21. Amerins MA, Ough CS. 1980. Methods for analysis of musts and wine. Wiley & Sons, New York, NY, USA. p 176-180.
  22. Sheu SJ, Tan YW. 1999. Determination of phenolic compound in Artemisia capillaris. J High Resolut Chromatogr 22: 222-224. https://doi.org/10.1002/(SICI)1521-4168(19990401)22:4<222::AID-JHRC222>3.0.CO;2-6
  23. Jung MJ, Yin Y, Heo SI, Wang MH. 2008. Antioxidant and anticancer activities of extract from Artemisia capillaries. Kor J Pharmacogn 39: 194-198.
  24. Yoshino M, Murakami K. 1998. Interaction of iron with polyphenolic compounds: application to antioxidant characterization. Anal Biochem 257: 40-44. https://doi.org/10.1006/abio.1997.2522
  25. Kim BY, Jeong JS, Kwon HJ, Lee JH, Hong SP. 2008. Determination of rosmarinic acid and caffeic acid from Perilla frutescens var. japonica and var. acuta by reversed phase HPLC. Kor J Herbology 23: 67-72.
  26. Park SK, Park JC. 1994. Antimicrobial activity of extracts and coumaric acid isolated from Artemisia princeps var. orientalis. Korean J Biotechnol Bioeng 9: 506-511.
  27. Rao SR, Ravishankar GA. 2000. Vanilla flavor: production by conventional and biotechnological routes. J Sci Food Agric 80: 289-304. https://doi.org/10.1002/1097-0010(200002)80:3<289::AID-JSFA543>3.0.CO;2-2
  28. Santosh Kumar S, Priyadarsini KI, Sainis KB. 2002. Free radical scavenging activity of vanillin and ο-vanillin using 1,1-diphenyl-2-prcrylhydrazyl (DPPH) radical. Redox Rep 7: 35-40. https://doi.org/10.1179/135100002125000163
  29. Burri J, Graf M, Lambelet P, Loliger J. 1989. Vanillin: more than a flavouring agent a potential antioxidant. J Sci Food Agric 48: 49-56. https://doi.org/10.1002/jsfa.2740480107
  30. Hong JH. 2011. Optimization of extraction conditions for functional components from Acai (Euterpe oleracea Mart.) by response surface methodology. J East Asian Soc Dietary Life 21: 713-722.
  31. Jeon SY, Baek JH, Jeong EJ, Cha YJ. 2012. Optimal extraction conditions of flavonoids from onion peels via response surface methodology. J Korean Soc Food Sci Nutr 41: 695-699. https://doi.org/10.3746/jkfn.2012.41.5.695
  32. Park KJ, Lim JH, Kim BK, Jeong JW, Kim JC, Lee MH, Cho YS, Jung HY. 2009. Optimization of extraction conditions to obtain functional components from buckwheat (Fagopyrum esculentum M.) sprouts, using response surface methodology. Korean J Food Preserv 16: 734-741.