DOI QR코드

DOI QR Code

Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

  • Cho, Moon Kyun (Department of Dermatology, Soonchunhyang University College of Medicine) ;
  • An, Je Min (Department of Dermatology, Soonchunhyang University College of Medicine) ;
  • Kim, Chul Han (Department of Plastic and Reconstructive Surgery, Soonchunhyang University College of Medicine) ;
  • Kang, Sang Gue (Department of Plastic and Reconstructive Surgery, Soonchunhyang University College of Medicine)
  • Received : 2013.05.30
  • Accepted : 2013.09.02
  • Published : 2014.01.15

Abstract

Background Aurora kinase A (Aurora-A) plays an important role in the regulation of mitosis and cytokinesis. Dysregulated Aurora-A leads to mitotic faults and results in pathological conditions. No studies on Aurora-A expression in human diabetic skin tissue have been reported. In light of this, we explored the expression of Aurora-A in human diabetic skin tissue. Methods Aurora-A protein was evaluated by western blotting in 6 human diabetic skin tissue and 6 normal skin specimens. Results Increased expression of Aurora-A protein was detected in all diabetic skin tissue samples in both western blot analysis and immunohistochemical staining. However, in the case of the normal skin tissue, no bands of Aurora-A protein were detected in either the western blotting analysis or the immunohistochemical staining. Conclusions Thus far, there have been no studies on the expression of Aurora-A in diabetic skin tissue. However, we believe that oxidative DNA damage related to the expression of Aurora-A protein and Aurora-A could be involved inhuman diabetic skin tissue.

Keywords

Acknowledgement

Supported by : Soonchunhyang University

References

  1. Girdler F, Gascoigne KE, Eyers PA, et al. Validating Aurora B as an anti-cancer drug target. J Cell Sci 2006;119:3664-75. https://doi.org/10.1242/jcs.03145
  2. Carmena M, Earnshaw WC. The cellular geography of aurora kinases. Nat Rev Mol Cell Biol 2003;4:842-54. https://doi.org/10.1038/nrm1245
  3. Li X, Sakashita G, Matsuzaki H, et al. Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C. J Biol Chem 2004;279:47201-11. https://doi.org/10.1074/jbc.M403029200
  4. Karthigeyan D, Prasad SB, Shandilya J, et al. Biology of Aurora A kinase: Implications in cancer manifestation and therapy. Med Res Rev 2010 Mar 1 [Epub]. http://dx.doi.org/10.1002/med.20203.
  5. West IC. Radicals and oxidative stress in diabetes. Diabet Med 2000;17:171-80. https://doi.org/10.1046/j.1464-5491.2000.00259.x
  6. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414:813-20. https://doi.org/10.1038/414813a
  7. Hsieh RH, Lien LM, Lin SH, et al. Alleviation of oxidative damage in multiple tissues in rats with streptozotocin-induced diabetes by rice bran oil supplementation. Ann N Y Acad Sci 2005;1042:365-71.
  8. Bhatia P, Menigatti M, Brocard M, et al. Mitotic DNA damage targets the Aurora A/TPX2 complex. Cell Cycle 2010; 9:4592-9. https://doi.org/10.4161/cc.9.22.13665
  9. Park HS, Park CH, Choi BR, et al. Expression of heat shock protein 105 and 70 in malignant melanoma and benign melanocytic nevi. J Cutan Pathol 2009;36:511-6. https://doi.org/10.1111/j.1600-0560.2008.01085.x
  10. Dutertre S, Descamps S, Prigent C. On the role of aurora-A in centrosome function. Oncogene 2002;21:6175-83. https://doi.org/10.1038/sj.onc.1205775
  11. Tsai MY, Wiese C, Cao K, et al. A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly. Nat Cell Biol 2003;5:242-8. https://doi.org/10.1038/ncb936
  12. Murata-Hori M, Tatsuka M, Wang YL. Probing the dynamics and functions of aurora B kinase in living cells during mitosis and cytokinesis. Mol Biol Cell 2002;13:1099-108.
  13. Mortlock AA, Foote KM, Heron NM, et al. Discovery, synthesis, and in vivo activity of a new class of pyrazoloquinazolines as selective inhibitors of aurora B kinase. J Med Chem 2007;50:2213-24. https://doi.org/10.1021/jm061335f
  14. Lengauer C, Kinzler KW, Vogelstein B. Genetic instabilities in human cancers. Nature 1998;396:643-9. https://doi.org/10.1038/25292
  15. Henle ES, Linn S. Formation, prevention, and repair of DNA damage by iron/hydrogen peroxide. J Biol Chem 1997;272: 19095-8. https://doi.org/10.1074/jbc.272.31.19095
  16. Qi M, Yu W, Liu S, et al. Septin1, a new interaction partner for human serine/threonine kinase aurora-B. Biochem Biophys Res Commun 2005;336:994-1000.
  17. Jackson SP, Bartek J. The DNA-damage response in human biology and disease. Nature 2009;461:1071-8.
  18. Krystyniak A, Garcia-Echeverria C, Prigent C, et al. Inhibition of Aurora A in response to DNA damage. Oncogene 2006;25:338-48. https://doi.org/10.1038/sj.onc.1209056
  19. Katayama H, Wang J, Treekitkarnmongkol W, et al. Aurora kinase-A inactivates DNA damage-induced apoptosis and spindle assembly checkpoint response functions of p73. Cancer Cell 2012;21:196-211. https://doi.org/10.1016/j.ccr.2011.12.025