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Molecular Characterization and Toxin Profile of Bacillus cereus Strains Isolated from Ready-to-eat Foods

유통 중인 즉석·편의식품류에서 분리한 Bacillus cereus의 산생 Toxin 및 분자유전학적 특성 조사

  • 김태순 (광주광역시 보건환경연구원) ;
  • 김민지 (광주광역시 보건환경연구원) ;
  • 강유미 (광주광역시 보건환경연구원) ;
  • 오그네 (광주광역시 보건환경연구원) ;
  • 최수연 (광주광역시 보건환경연구원) ;
  • 오무술 (광주광역시 보건환경연구원) ;
  • 양용식 (광주광역시 보건환경연구원) ;
  • 서정미 (광주광역시 보건환경연구원) ;
  • 류미금 (광주광역시 보건환경연구원) ;
  • 김은선 (광주광역시 보건환경연구원) ;
  • 하동룡 (광주광역시 보건환경연구원) ;
  • 조배식 (광주광역시 보건환경연구원)
  • Received : 2014.04.04
  • Accepted : 2014.05.02
  • Published : 2014.06.30

Abstract

Toxin-producing Bacillus cereus is the causative agent of two different types of food poisoning: the emetic and the diarrheal types. This study was conducted to investigate the presence of enterotoxin and emetic toxin genes in 263 B. cereus isolated from 619 different ready-to-eat food items. Hemolytic enterotoxins hblA, hblC, and hblD were detected in 85.6, 41.1, and 76.8%, respectively, of the B. cereus isolates. About 67.0% (175/263) of the isolates presented all of three genes. Non-hemolytic enterotoxins nheA, nheB, and nheC were detected in 100, 97.0, and 68.4% of the isolates, respectively. Approximately 90.0% (236/263) of the isolates presented all of these three non-hemolytic enterotoxin genes. Emetic toxin gene, CER, was detected in 132 of 263 (50.2%) isolates. Computer-assisted cluster analysis of Rep-PCR profiles showed a high genetic diversity among the isolates. All B. cereus isolates from food samples tested in this study carried at least 6 of 10 toxin genes.

Keywords

B. cereus;enterotoxin;emetic toxin;PCR;genetic diversity

Acknowledgement

Supported by : 광주광역시보건환경연구원

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