DOI QR코드

DOI QR Code

Antimicrobial Chitosan-silver Nanocomposite Film Prepared by Green Synthesis for Food Packaging

녹색합성법에 기인한 식품포장용 키토산-은나노 항균 복합필름의 개발

  • Received : 2014.01.24
  • Accepted : 2014.02.16
  • Published : 2014.06.30

Abstract

We studied the green synthesis and antibacterial activity of chitosan-silver (Ag) nanocomposite films for application in food packaging. Green synthesis of Ag nanoparticles (AgNPs) was achieved by a chemical reaction involving a mixture of chitosan-silver nitrate ($AgNO_3$) in an autoclave at 0.1 MPa, $121^{\circ}C$, for 15-120 s. The formation of AgNPs in chitosan was confirmed by both UV-Visible spectrophotometry and transmission electron microscopy (TEM) and the effects of chitosan-$AgNO_3$ concentration and reaction time on the synthesis of AgNPs in chitosan were examined. The resulting chitosan-Ag composite films were characterized by various analytical techniques and their antibacterial activity was evaluated based on the formation of halo zones around films, indicating inhibition of the growth of Escherichia coli. A fourier-transform infrared (FTIR) spectroscopy analysis showed that free amino groups in chitosan acted as effective reductants and AgNP stabilizers. The composite films exhibited enhanced antibacterial activity with increasing Ag content on the surface of as-prepared composite films.

Keywords

green synthesis;chitosan;silver nanoparticle;antimicrobial activity;food packaging

References

  1. Coma V, Martial-Gros A, Garreau S, Copinet A, Salin F, Deschamps A. Edible antimicrobial films based on chitosan matrix. J. Food Sci. 67: 1162-1169 (2002) https://doi.org/10.1111/j.1365-2621.2002.tb09470.x
  2. Wei D, Sun W, Qian W, Ye Y, Ma X. The synthesis of chitosanbased silver nanoparticles and their antibacterial activity. Carbohyd. Res. 344: 2375-2382 (2009) https://doi.org/10.1016/j.carres.2009.09.001
  3. Wei D, Ye Y, Jia X, Yuan C, Qian W. Chitosan as an active support for assembly of metal nanoparticles and application of the resultant bioconjugates in catalysis. Carbohyd. Res. 345: 74-81 (2010) https://doi.org/10.1016/j.carres.2009.10.008
  4. Abdollahi M, Rezaei M, Farzi G. A novel active bionanocomposite film incorporating rosemary essential oil and nanoclay into chitosan. J. Food Eng. 111: 343-350 (2012) https://doi.org/10.1016/j.jfoodeng.2012.02.012
  5. Park SC, Kang JH, Lim HA. Study on the change in physical and functional properties of paper by the addition of chitosan. J. Korea TAPPI 42: 37-46 (2010)
  6. Bordenave N, Grelier S, Coma V. Hydrophobization and antimicrobial activity of chitosan and paper-based packaging material. Biomacromolecules 11: 88-96 (2010) https://doi.org/10.1021/bm9009528
  7. Pinto RJB, Fernandes SCM, Freire CSR, Sadocco P, Causio J, Neto CP, Trindade T. Antibacterial activity of optically transparent nanocomposite films based on chitosan or its derivatives and silver nanoparticles. Carbohyd. Res. 348: 77-83 (2012) https://doi.org/10.1016/j.carres.2011.11.009
  8. Ravi Kumar MNV. A review of chitin and chitosan applications. React. Funct. Polym. 46: 1-27 (2000) https://doi.org/10.1016/S1381-5148(00)00038-9
  9. ASTM. Standard test method for determining the antimicrobial activity of immobilized antimicrobial agents under dynamic contact conditions (E 2149-10). American Society for Testing and Materials, West Conshohocken, PA, USA (2010)
  10. Venkatesham M, Ayodhya D, Madhusudhan A, Babu NV, Veerabhadram G. A novel green one-step synthesis of silver nanoparticles using chitosan: catalytic activity and antimicrobial studies. Appl. Nanosci. 4: 113-119 (2014) https://doi.org/10.1007/s13204-012-0180-y
  11. Wei D, Qian W. Facile synthesis of Ag and Au nanoparticles utilizing chitosan as a mediator agent. Colloid. Surface. B 62: 136-142 (2007)
  12. Hwang IS, Cho JY, Hwang JH, Hwang BM, Choi HM, Lee JY, Lee DG. Antimicrobial effects and mechanism(s) of silver nanoparticle. Korean J. Microbiol. Biotechnol. 39: 1-8 (2011)
  13. Courrol LC, Silva FRO, Gomes L. A simple method to synthesize silver nanoparticles by photo-reduction. Colloid. Surface. A 305: 54-57 (2007) https://doi.org/10.1016/j.colsurfa.2007.04.052
  14. Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M. Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomed.-Nanotechnol. 5: 382-386 (2009) https://doi.org/10.1016/j.nano.2009.06.005
  15. Ghaseminezhad SM, Hamedi S, Shojaosadati SA. Green synthesis of silver nanoparticles by a novel method: comparative study of their properties. Carbohyd. Polym. 89: 467-472 (2012) https://doi.org/10.1016/j.carbpol.2012.03.030
  16. Vidhu VK, Aswathy Aromal S, Philip D. Green synthesis of silver nanoparticles using Macrotyloma uniflorum. Spectrochim. Acta A 83: 392-397 (2011) https://doi.org/10.1016/j.saa.2011.08.051
  17. Appendini P, Hotchkiss JH. Review of antimicrobial food packaging. Innov. Food Sci. Emerg. 3: 113-126 (2002) https://doi.org/10.1016/S1466-8564(02)00012-7
  18. Cho JH, Lee YW, Kim HJ, Lee JM. Study on preparation of environmental-friendly specialty paper using functional antibiotic nano-particle (I). Appl. Chem. Eng. 16: 385-390 (2005)
  19. Sung SY, Sin LT, Tee TT, Bee ST, Rahmat AR, Rahman WAWA, Tan AC, Vikhraman M. Antimicrobial agents for food packaging applications. Trends Food Sci. Tech. 33: 110-123 (2013) https://doi.org/10.1016/j.tifs.2013.08.001
  20. Weng YM, Hotchkiss JH. Anhydrides as antimycotic agents added to polyethylene films for food packaging. Packag. Technol. Sci. 6: 123-128 (1993) https://doi.org/10.1002/pts.2770060304
  21. Muriel-Galet V, Cerisuelo JP, Lopez-Carballo G, Lara M, Gavara R, Hernandez-Munoz P. Development of antimicrobial films for microbiological control of packaged salad. Int. J. Food Microbiol. 157: 195-201 (2012) https://doi.org/10.1016/j.ijfoodmicro.2012.05.002
  22. Kim JY, Kim TY, Yoon JY. Antimicrobial activity and mechanism of silver. Appl. Chem. Eng. 20: 251-257 (2009)
  23. Cruz-Romero MC, Murphy T, Morris M, Cummins E, Kerry JP. Antimicrobial activity of chitosan, organic acids and nano-sized solubilisates for potential use in smart antimicrobially-active packaging for potential food applications. Food Control 34: 393-397 (2013) https://doi.org/10.1016/j.foodcont.2013.04.042
  24. Cho JH, Lee YW, Kim HJ. Study on preparation of environmental-friendly specialty paper using functional antibiotic nano-particle (II). Appl. Chem. Eng. 18: 17-23 (2007)
  25. Gonzalez A, Alvarez Igarzabal CI. Soy protein-poly (latic acid) bilayer films as biodegradable material for active food packaging. Food Hydrocolloid. 33: 289-296 (2013) https://doi.org/10.1016/j.foodhyd.2013.03.010
  26. Seydim AC, Sarikus G. Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Res. Int. 39: 639-644 (2006) https://doi.org/10.1016/j.foodres.2006.01.013