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Nutritional Components of Zespri Green Kiwi Fruit (Actinidia delicosa) and Neuronal Cell Protective Effects of the n-hexane fraction

제스프리 그린 키위의 주요 영양성분 및 헥산 분획물의 신경세포 보호효과

  • Jin, Dong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Hyeon Ju (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Ji Hee (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jo, Yu Na (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kwon, O-Jun (Daegyeong Institute for Regional Program Evaluation, Regional Industry Evaluation Agency for Gyeongbuk) ;
  • Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 진동은 (경상대학교 응용생명과학부) ;
  • 김현주 (경상대학교 응용생명과학부) ;
  • 정지희 (경상대학교 응용생명과학부) ;
  • 조유나 (경상대학교 응용생명과학부) ;
  • 권오준 (경북지역사업평가단) ;
  • 최성길 (경상대학교 응용생명과학부) ;
  • 허호진 (경상대학교 응용생명과학부)
  • Received : 2013.12.24
  • Accepted : 2014.02.25
  • Published : 2014.06.30

Abstract

The physiological characteristics of kiwi (Actinidia delicosa) fruit were analyzed, which inclued its nutritional composition, in vitro-antioxidant activities, and neuronal cell protective effects. The most abundant components of mineral, amino acid, and fatty acid were found to be potassium (K), glutamic acid, and a-linolenic acid, respectively. The major free sugars were fructose, glucose, and sucrose. In addition, ${\beta}$-carotene and vitamin C contents were $1.35{\mu}g/100mL$ and 29.21 mg/100 g, respectively. The 2,2'-azinobis-(3-ethylbenothiazline-6-sulfonic acid) (ABTS) radical-scavenging activity of the n-hexane fraction obtained from the kiwi extract was 10.52% at a concentration of $1000{\mu}g/mL$. The malondialdehyde (MDA) inhibition of the n-hexane fraction was found to increased in a dose-dependent manner. The intracellular reactive oxygen species (ROS) accumulation after hydrogen peroxide ($H_2O_2$) treatment of PC12 cells was significantly reduced in the presence of the n-hexane fraction compared to PC12 cells treated with $H_2O_2$ only. Moreover, in the a MTT assay, the n-hexane fraction showed in vitro-protective effects against $H_2O_2$-induced neurotoxicity.

Keywords

Green kiwi fruit;Actinidai delicosa;neuronal protection;PC12 cell;antioxidant

Acknowledgement

Supported by : 한국연구재단, 산림청

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