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Protective Effect of Radiation-induced New Blackberry Mutant γ-B201 on H2O2-induced Oxidative Damage in HepG2 Cells

H2O2 에 의해 유도된 HepG2 세포의 산화적 스트레스에 대한 신품종 방사선 돌연변이 블랙베리 γ-B201의 세포 보호 효과

  • Cho, Byoung Ok (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Chang-Wook (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • So, Yangkang (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jin, Chang-Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Yook, Hong-Sun (Department of Food and Nutrition, Chungnam National University) ;
  • Byun, Myung-Woo (Department of Culinary Nutrition, Woosong University) ;
  • Jeong, Yong-Wook (Department of Microbiology, College of Medicine, Seonam University) ;
  • Park, Jong Chun (Department of Microbiology, College of Medicine, Seonam University) ;
  • Jeong, Il-Yun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 조병옥 (한국원자력연구원 첨단방사선연구소) ;
  • 이창욱 (한국원자력연구원 첨단방사선연구소) ;
  • 소양강 (한국원자력연구원 첨단방사선연구소) ;
  • 진창현 (한국원자력연구원 첨단방사선연구소) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 변명우 (우송대학교 외식조리영양학부) ;
  • 정용욱 (서남대학교 의과대학 미생물학교실) ;
  • 박종천 (서남대학교 의과대학 미생물학교실) ;
  • 정일윤 (한국원자력연구원 첨단방사선연구소)
  • Received : 2014.01.28
  • Accepted : 2014.04.04
  • Published : 2014.06.30

Abstract

The objective of the present study was to investigate the chemical composition of anthocyanin-enriched extract of radiation-induced blackberry (Rubus fruticosus L.) mutant (${\gamma}$-B201) as well as the protective effect of ${\gamma}$-B201 against oxidative stress in vitro. The cytotoxicity, reactive oxygen species (ROS) scavenging capacity, and DNA damage were assessed by WST-1 assay, flow cytometry, and comet assay, respectively. Lactate dehydrogenase, superoxide dismutase, and catalase activities were determined by using a commercial kit. The in vitro results showed that ${\gamma}$-B201 increased the cell viability, reduction of lactate dehydrogenase release, and intracellular ROS scavenging capacity in hydrogen peroxide ($H_2O_2$)-treated HepG2 cells. Furthermore, treatment with ${\gamma}$-B201 attenuated DNA damage in $H_2O_2$-treated HepG2 cells and treatment with ${\gamma}$-B201 restored the activity of superoxide dismutase and catalase in $H_2O_2$-treated HepG2 cells. In conclusion, the present study suggests that ${\gamma}$-B201 blackberry extract can exert a significant cytoprotective effect against $H_2O_2$-induced cell damage.

Keywords

blackberry;ROS;cell viability;DNA damage;antioxidant enzyme

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