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Protective Effects of Radiation-induced Blackberry Mutant Extract on Carbon Tetrachloride (CCl4)-induced Liver Injury in Sprague-Dawley Rats

방사선 돌연변이 블랙베리 추출물의 사염화탄소(Carbon Tetrachloride, CCl4) 투여에 의한 흰쥐의 간 손상에 대한 보호 효과

  • Cho, Byoung Ok (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • Lee, Chang-Wook (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • So, Yangkang (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • Jin, Chang-Hyun (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University) ;
  • Byun, Myung-Woo (Dept. of Culinary Nutrition, Woosong University) ;
  • Jeong, Yong-Wook (Dept. of Microbiology, College of Medicine, Seonam University) ;
  • Park, Jong Chun (Dept. of Microbiology, College of Medicine, Seonam University) ;
  • Jeong, Il-Yun (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute)
  • 조병옥 (한국원자력연구원 첨단방사선연구소) ;
  • 이창욱 (한국원자력연구원 첨단방사선연구소) ;
  • 소양강 (한국원자력연구원 첨단방사선연구소) ;
  • 진창현 (한국원자력연구원 첨단방사선연구소) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 변명우 (우송대학교 외식조리영양학부) ;
  • 정용욱 (서남대학교 의과대학 미생물학교실) ;
  • 박종천 (서남대학교 의과대학 미생물학교실) ;
  • 정일윤 (한국원자력연구원 첨단방사선연구소)
  • Received : 2014.02.05
  • Accepted : 2014.04.08
  • Published : 2014.06.30

Abstract

The objective of the present study was to investigate the protective effects of anthocyanin-enriched extract from radiation-induced blackberry (Rubus fruticosus L.) mutant (${\gamma}$-B201) against carbon tetrachloride ($CCl_4$)-induced liver injury in Spargue-Dawley (SD) rats. The in vivo results show that ${\gamma}$-B201 attenuated the levels of serum aspartate aminotransferase, alanine aminotransferase, and liver lipid peroxidation in $CCl_4$-treated SD rats. Histopathological examination of rat livers showed that ${\gamma}$-B201 reduced the incidence of liver lesions induced by $CCl_4$. Moreover, ${\gamma}$-B201 prevented DNA damage in $CCl_4$-treated SD rats. Furthermore, administration of ${\gamma}$-B201 significantly increased the activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), in $CCl_4$-treated rat livers. In conclusion, the present study suggests that ${\gamma}$-B201 blackberry extract protects the liver from $CCl_4$-induced hepatic damage through an antioxidant mechanism. Therefore, ${\gamma}$-B201 blackberry may be functional food material for human health.

Acknowledgement

Supported by : 미래창조과학부

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