Antioxidant Activities of Pleurotus cornucopiae Extracts by Extraction Conditions

추출조건에 따른 노랑느타리버섯 추출물의 항산화 활성

  • Received : 2014.02.03
  • Accepted : 2014.03.03
  • Published : 2014.06.30


Physiological activities of extracts form Pleurotus cornucopiae were examined. DPPH radical scavenging activity of water extract was 70.22 percent at 4 mg/mL, which was the highest among all extracts. Total polyphenol content of water extract was 26.34 mg/g, which was the highest value for all extraction conditions. Superoxide anion radical scavenging activities ranged from 50.17 to 80.56 percent at 1, 2, and 4 mg/mL, which were higher than the ascorbic acid activity (56.19% at 10 mg/mL) (P<0.05). ACE inhibitory activities were higher in ethanolic extracts than in water extracts. Nitrite-scavenging abilities under acidic conditions (pH 1.2 and pH 3.0) were the most effective among all the extracts. These results will be useful for understanding the physiological activities of Pleurotus cornucopiae.


  1. Kim HS, Ha HC, Kim TS. 2003. Research and prospects in new functional mushroom-Tremella fuciformis, Grofora frondosa, and Hypsizigus marmoreus-. Food Sci Ind 36(4):42-46.
  2. Yang HC, Song CH, Kweon MH. 1996. Mycelial new material, food functional technology. Hanlimwon, Seoul, Korea. p 187-189.
  3. Jo EK. 2012. Physiological and antioxidant activities of subcritical water extracts from gold oyster mushroom (Pleurotus cornucopiae Rolland var. citrinopileatus). MS Thesis. Kyungnam University, Changwon, Korea. p 38-39.
  4. Hossain S, Hashimoto M, Choudhury EK, Alam N, Hussain S, Hasan M, Choudhury SK, Mahmud I. 2003. Dietary mushroom (Pleurotus ostreatus) ameliorates atherogenic lipid in hypercholesterolaemic rats. Clin Exp Pharmacol Physiol 30: 470-475.
  5. Barrosa L, Baptistaa P, Correiaa DM, Casalb S, Oliveirab B, Ferreira ICFR. 2007. Fatty acid and sugar compositions, and nutritional value of five wild edible mushrooms from Northeast Portugal. Food Chem 105: 140-145.
  6. Jung IC, Park S, Park KS, Ha HC, Kim SH, Kwon YI, Lee JS. 1996. Antioxidative effect of fruit body and mycelial extracts of Pleurotus ostreatus. Korean J Food Sci Technol 28: 464-469.
  7. Kim MS, Kim GH. 2010. Contents of nucleic acids (nucleosides and mono-nuleotides) in extracts of Pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes. Korean J Food & Nutr 23: 376-380.
  8. Kim HJ, Bae JT, Lee JW, HwangBo MH, Im HK, Lee IS. 2005. Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extracts. Korean J Food Preserv 12: 80-85.
  9. Qi Y, Zhao X, Lim YI, Park KY. 2013. Antioxidant and anticancer effects of edible and medicinal mushrooms. J Korean Soc Food Sci Nutr 42: 655-662.
  10. Yoo BH. 2011. Production of ${\beta}$-glucan using mixed culture of mushroom mycelium and Trichoderma koningii. MS Thesis. Inje University, Gimhae, Korea. p 4-5.
  11. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1120.
  12. Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.
  13. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European J Biochem 47: 468-474.
  14. Cushman DW, Chung HS. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem Pharmacol 20: 1637-1648.
  15. Gray JI, Dugan Jr LR. 1975. Inhibition of N-nitrosamine formation in model food systems. J Food Sci 40: 981-984.
  16. Cho ML, Lee DJ, You SG. 2012. Radical scavenging activity of ethanol extracts and solvent partitioned fractions from various red seaweeds. Ocean and Polar Research 34: 445-451.
  17. Torel J, Cillard J, Cillard P. 1986. Antioxidant activity of flavoniods and reactivity with peroxy radical. Phytochemistry 25: 383-385.
  18. Kang HW. 2012. Antioxidant and anti-inflammatory effect of extracts from Flammulina velutipes (Curis) singer. J Korean Soc Food Sci Nutr 41: 1072-1078.
  19. Kim HJ, Bae JT, Lee JW, Hwangbo MH, Im HG, Lee IS. 2005. Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extracts. Korean J Food Preserv 12: 80-85.
  20. Lee YS, Joo EY, Kim NW. 2006. Polyphenol contents and antioxidant activity of Lepista nuda. J Korean Soc Food Sci Nutr 35: 1309-1314.
  21. Qi Y, Zhao X, Lim YL, Park KY. 2013. Antioxidant and anticancer effects of edible and medicinal mushrooms. J Korean Soc Food Sci Nutr 42: 655-662.
  22. Wang SY, Chang HN, Lin KT, Lo CP, Yang NS, Shyur LF. 2003. Antioxidant properties and phytochemical characteristics of extracts from Lactuca indica. J Agric Food Chem 26: 1506-1512.
  23. Ha JH, Jeong MH, Seo YC, Yong CW, Kim JS, Kim HH, Ahn JH, Lee HY. 2010. Enhancement of antioxidant activities of bark of Berberis koreana Palibin by lactic acid fermentation. Korean J Medicinal Crop Sci 18: 421-428.
  24. Kim DH, Park SR, Debnath T, Hasnat MA, Mehnaz P, Lim BO. 2013. Evaluation of the antioxidant activity and anti-inflammatory effect of Hericium erinaceus water extracts. Korean J Medicinal Crop Sci 21: 112-117.
  25. Cushman DW, Ondetti MA. 1980. Inhibitors of angiotensinconverting enzyme for treatment of hypertension. Biochem Pharmacol 29: 1871-1877.
  26. Song JH, Lee HS, Hwang JK, Han JW, Ro JG, Keum DH, Park KM. 2003. Physiological activity of Sarcodon aspratus extracts. Korean J Food Sci Ani Resour 23: 172-179.
  27. Song JH, Lee HS, Hwang JK, Chung TY, Hong SR, Park KM. 2003. Physiological activities of Phellinus ribis extracts. Korean J Food Sci Technol 35: 690-695.
  28. Park WM, Kim GH, Hyeon JW. 1995. New synthetic medium for growth of mycelium of Pleurotus species. Korean J Mycol 23: 275-283.
  29. Leaf CD, Vecchio AJ, Roe DA, Hotchkiss JH. 1987. Influence of ascorbic acid dose on N-nitrosoproline formation in humans. Carcinogenesis 8: 791-795.
  30. Kwon SC. 2011. Biological activities of ethanol extracts from Hericium erinaceus mycelium on Angelica keiskei and Angelica keiskei pomace. J Korean Soc Food Sci Nutr 40:1648-1653.

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