A Pilot Study on Single-dose Toxicity Testing of Scolopendrid Pharmacopuncture in Sprague-Dawley Rats

  • Son, Ilhong (Department of Neurology, Inam Neuroscience Research Center, Sanbon Medical Center, College of Medicine, Wonkwang University) ;
  • Lee, Sangmi (ALS/MND Center of Wonkwang University) ;
  • Kim, Doho (ALS/MND Center of Wonkwang University) ;
  • Jeong, Hohyung (ALS/MND Center of Wonkwang University) ;
  • Cho, Seung-Hun (Department of Neuropsychiatry, Hospital of Korean Medicine, Kyung Hee University Medical center) ;
  • Lee, Eun-Yong (Department of Acupuncture & Moxibustion Medicine, Semyung University Korean Medical Hospital) ;
  • Lee, Seung-Deok (Department of Acupuncture & Moxibustion Medicine, Dongguk University College of Korean Medicine) ;
  • Ahn, Seong-Hun (Department of Meridian & Acupoint, Wonkwang University College of Korean Medicine) ;
  • Kim, Sungchul (ALS/MND Center of Wonkwang University)
  • Received : 2013.05.29
  • Accepted : 2014.01.13
  • Published : 2014.06.30


Objectives: This study was performed to analyze single dose toxicity and the lethal dose of Scolopendrid Pharmacopuncture in rats. Methods: All experiments were conducted at the Korea Testing & Research Institute (KTR), an institution authorized to perform non-clinical studies, under the regulations of Good Laboratory Practice (GLP). Sprague-Dawley rats were chosen for the pilot study. Doses of Scolopendrid pharmacopuncture, 0.1, 0.5, and 1.0 mL, were administered to the experimental group, and 1.0 mL doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethic Committee. Results: No deaths or abnormalities occurred in any of the groups. No significant changes in the weight, hematological parameters or clinical chemistry were noted between the control group and the experimental group. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs or tissues. Conclusion: The above findings suggest Scolopendrid Pharmacopuncture is a relatively safe to use for treatment. Further studies on the subject should be conducted to yield more concrete evidence.


Supported by : Ministry for Health & Welfare


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