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Study of improving precision and accuracy by using an internal standard in post column isotope dilution method for HPLC-ICP/MS

후 컬럼 동위원소 희석법을 적용한 HPLC-ICP/MS에서의 정량분석에서 내부 표준물을 이용한 정확도와 정밀도의 개선연구

  • Joo, Mingyu (Department of Chemistry Education, Korea National University of Education Cheng-Won) ;
  • Park, Myungsun (Department of Chemistry Education, Korea National University of Education Cheng-Won) ;
  • Pak, Yong-Nam (Department of Chemistry Education, Korea National University of Education Cheng-Won)
  • 주민규 (한국교원대학교 화학교육학과) ;
  • 박명순 (한국교원대학교 화학교육학과) ;
  • 박용남 (한국교원대학교 화학교육학과)
  • Received : 2014.04.08
  • Accepted : 2014.06.09
  • Published : 2014.06.25

Abstract

An internal standard was used in PCID (post column isotope dilution) to improve the accuracy and precision in quantification of various chemical species. The error occurring in the column was the largest in HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry) when PCID and other traditional quantification methods were compared with each other. Internal standard was effective in correcting the loss of sample in the column to improve accuracy and precision. When applied to SeMet, using MeSecys or $Se^{4+}$ as an internal standard, relative errors were reduced from 31% and 13% to less than 1%, while standard deviations were reduced from 5.1% and 6.9% to 1.5% and 0.2%, respectively. Positive aspects of using an internal standard in PCID were compared with other quantitative techniques and discussed in detail.

Keywords

internal standard;post column isotope dilution;species unspecific ID;HPLC-ICP/MS

Acknowledgement

Supported by : 한국연구재단

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