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Fungicidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient against Trichophyton mentagrophytes, Candida albicans and Aspergillus niger

Ortho-phenylphenol을 주성분을 하는 훈증소독제의 Trichophyton mentagrophytes, Candida albicans 그리고 Aspergillus niger에 대한 살진균 효과

  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Lee, Soo-Ung (Chuncheon Bioindustry Foundation) ;
  • Cho, Ki-Yung (Meteorology education platoon, Information & Communication School, ROKAF Education & Training Command) ;
  • Kim, Yongpal (Elkahmco Bio Co., Ltd.) ;
  • Yoo, Chang-Yeol (Department of Computer Information, Gyeongnam Provincial Namhae College) ;
  • Kim, Suk (Research Institute of Life Sciences and College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Research Institute of Life Sciences and College of Veterinary Medicine, Gyeongsang National University)
  • 박은기 (고신대학교 의과대학 인문사회의학교실) ;
  • 이수응 ((재)춘천바이오산업진흥원) ;
  • 조기융 (공군교육사령부 정보통신학교 기상교육대) ;
  • 김용팔 (엘캄코 바이오(주)) ;
  • 유창열 (경남도립남해대학 인터넷정보학과) ;
  • 김석 (경상대학교 수의과대학.생명과학연구원) ;
  • 이후장 (경상대학교 수의과대학.생명과학연구원)
  • Received : 2014.04.03
  • Accepted : 2014.06.12
  • Published : 2014.06.30

Abstract

Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenol against Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillus niger (A. niger). Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant were left in a sealed room ($25m^3$) at $21{\pm}0.5^{\circ}C$ and $60{\pm}10%$ relative humidity for 15 hours. Immediately after removal from the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated. Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were $1.0{\times}10^8$, $1.2{\times}10^8$ and $5.7{\times}10^7CFU/mL$, respectively. All the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2 (the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all mean numbers of test strains recovered on the control-carriers (T value) were over $10^6CFU/mL$. For the fungicidal effect of the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL. Conclusions: The present study showed that fumigant containing 20% ortho-phenylphenol has effective fungicidal activity against T. mentagrophytes, C. albicans and A. niger.

Keywords

Aspergillus niger;Candida albicans;Fumigation disinfectant;Ortho-phenylphenol;Trichophyton mentagrophytes

Acknowledgement

Supported by : (주)엘캄코바이오

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