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Development of CAPS marker for identifying a Formosan lily (Lilium formosanum)

흰나리(Lilium formosanum Wallace) 식별을 위한 CAPS 마커의 개발

  • Chung, Sung Jin (Department of Horticulture, Chungnam National University) ;
  • Lee, Ka Youn (Department of Horticulture, Chungnam National University) ;
  • Yoon, A Ra (Department of Horticulture, Chungnam National University) ;
  • Jang, Ji Young (Department of Horticulture, Chungnam National University) ;
  • Kim, Jin Kug (National Agricultural Products Quality Management Service) ;
  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University)
  • 정성진 (충남대학교 농업생명과학대학 원예학과) ;
  • 이가연 (충남대학교 농업생명과학대학 원예학과) ;
  • 윤아라 (충남대학교 농업생명과학대학 원예학과) ;
  • 장지영 (충남대학교 농업생명과학대학 원예학과) ;
  • 김진국 (국립농산물품질관리원 충남지원) ;
  • 이긍주 (충남대학교 농업생명과학대학 원예학과)
  • Received : 2014.06.16
  • Accepted : 2014.06.30
  • Published : 2014.06.30

Abstract

This study was conducted to identify lily species native to Korea from formosan lily (Lilium formosanum) belonging to Longiflorum section. Due to flowering time, flower color and orientation, long shelf life and resistant to diseases, the native lily species can be valuable genetic resources for interspecific hybrids. One of the chloroplast genes, matK, was used to clone and sequence to explore any base changes. The matK was successfully amplified into 1,539 bp (94% of the gene) and phylogenetic tree demonstrated 6 clades for those 11 lily species used in this study. There were one or two base substitutions among 10 lilies native to Korea, while formosan lily native to Taiwan exhibited 6 base substitutions in matK gene, rendering it genetically distant. A restriction enzyme NruI recognized one of the six base changes, and digested the matK gene of 10 native lily species only, but not in formosan lily. The confirmed cleavage characteristic of the target region in matK gene was designed into a CAPS (cleaved amplified polymorphic sequences) marker which will be available to estimate compatibility of interspecific hybridization and to trace the pedigree when those native lilies are crossed with the formosan lily.

Acknowledgement

Supported by : 농림수산식품기술기획평가원

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