Analysis of carotenoids and soluble sugars in the Rainbow carrots

무지개 당근의 carotenoid와 당 함량 분석

  • Kim, Sa-Rang (Department of Food and Nutrition, Chungnam National University) ;
  • Kim, Yeun-Mi (Department of Food Science and Technology, Chungnam National University) ;
  • Jeon, Sang-Jin (Breading Research Institute, Carrotop Seed Co.) ;
  • Park, Jong-Tae (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, Jae-Han (Department of Food and Nutrition, Chungnam National University)
  • 김사랑 (충남대학교 식품영양학과) ;
  • 김연미 (충남대학교 식품공학과) ;
  • 전상진 ((주)캐로톱시드 영농조합) ;
  • 박종태 (충남대학교 식품공학과) ;
  • 김재한 (충남대학교 식품영양학과)
  • Received : 2013.04.30
  • Accepted : 2014.04.21
  • Published : 2014.06.30


Coloring agents in food materials plays important roles in the development of attractive products as well as in the functionality of food such as antioxidant or vitamin supplementation. Carrot has been used as an orange coloring agent in the decoration of food but also a major source of vitamin A complex. Though orange has been considered a typical color of carrot, the Rainbow carrot has been developed recently, which exhibit the various colors such as red, pale yellow, purple, orange or their mixtures. After categorization onto 8 groups by their colors, vitamin A complex (${\beta}$-carotene, lycopene and lutein) and soluble sugars (glucose, fructose, and sucrose) have been analyzed in carrots. The ${\beta}$-carotene was abundant in the groups of orange (Group-O) or groups with the orange color (group-OP, and group-YOP). The content of lycopene content was exclusively high in the red color carrot (group-R). The highest lutein contents were observed from the yellow-purple (group-YP) group. Meanwhile, little amounts of lycopene and ${\beta}$-carotene were observed in yellow-purple (group-YP) nor yellow (group-Y) on yellow (group-Y). Among the reducing sugars in 'rainbow carrots', the amount of sucrose was two times higher than those of fructose and glucose. However, the content of glucose, fructose and sucrose as well as the total reducing sugars did not differ between color groups suggesting little variations on their tastes.


Supported by : 충남대학교


  1. Heinonen M. I. 1990. Carotenoids and Provitamin-a Activity of Carrot (Daucus-Carota L) Cultivars. Journal of Agricultural and Food Chemistry 38(3):609-612.
  2. Matthews PD. Luo RB. 2003. Maize phytoene desaturase and zeta-carotene desaturase catalyse a poly-Z desaturation pathway: implications for genetic engineering of carotenoid content among cereal crops. Journal of Experimental Botany 54(391):2215-2230.
  3. Nilsson T. 1987. Growth and Chemical-Composition of Carrots as Influenced by the Time of Sowing and Harvest. Journal of Agricultural Science 108:459-468.
  4. Nookaraju A, Upadhyaya CP. 2010. Molecular approaches for enhancing sweetness in fruits and vegetables. Scientia Horticulturae 127(1):1-15.
  5. Simon PW, Peterson CE. lindsay RC. 1980. Correlations between sensory and objective parameters of carrot flavor. Journal of Agricultural and Food Chemistry 28(3):559-562.
  6. Vilchez C. Forjan E. 2011. "Marine carotenoids: biological functions and commercial applications." Marine Drugs 9(3): 319-333.
  7. Yuan D, Bassie L. 2011. The potential impact of plant biotechnology on the Millennium Development Goals. Plant Cell Reports 30(3):249-265.
  8. Zhu CF, Naqvi S. 2008. Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize. Proceedings of the National Academy of Sciences of the United States of America 105(47): 18232-18237.
  9. Zhu CF, Bai C. 2010. The regulation of carotenoid pigmentation in flowers. Archives of Biochemistry and Biophysics 504(1): 132-141.
  10. Baranski R, Allender C, and Klimek-Chodacka M. 2012. Towards better tasting and more nutritious carrots: Carotenoid and sugar content variation in carrot genetic resources. Food Research International 47(2):182-187.
  11. Barret G, Fabi J. Rosso V. Cordenunsi B. Lajoloo F. Nascimento J. Mercadante A. 2011. Influence of ethylene on carotenoid biosynthesis during papaya postharvesting ripening. Journal of Food Composition and Analysis 24(4-5):620-624.
  12. Bendich A. J. A. Olson 1989. Biological actions of carotenoids. The journal of the Federation of American Societies for Experimental Biology 3(8):1927-1932.
  13. Britton G, Weesie RJ. Askin D. Warburton JD. GallardoGuerrero L. Jansen FJ. deGroot HJM. Lugtenburg J. Cornard JP. Merlin JC. 1997. Carotenoid blues: Structural studies on carotenoproteins. Pure and Applied Chemistry 69(10):2075-2084.
  14. Daie J. 1984. Characterization of sugar transport in storage tissue of carrot. Journal of the American Society for Horticultural Science 109:718-722.
  15. Furr HC. 2004. Analysis of retinoids and carotenoids: problems resolved and unsolved. Journal of Nutrition 134(1):281S-285S.
  16. Gueguen S, Herbeth B. 2002. An isocratic liquid chromatographic method with diode-array detection for the simultaneous determination of alpha-tocopherol, retinol, and five carotenoids in human serum. Journal of Chromatographic Science 40(2): 69-76.