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Fabrication and Evaluation of Hybrid Scaffold by Nano-Micro Precision Deposition System

나노-마이크로 정밀 분사 시스템을 이용한 하이브리드 인공지지체의 제작 및 평가

  • Ha, Seong-Woo (Dept. of Mechanical Engineering, Andong Nat'l Univ.) ;
  • Kim, Jong Young (Dept. of Mechanical Engineering, Andong Nat'l Univ.)
  • 하성우 (안동대학교 기계공학과) ;
  • 김종영 (안동대학교 기계공학과)
  • Received : 2014.04.17
  • Accepted : 2014.05.30
  • Published : 2014.08.01

Abstract

Recently, three-dimensional scaffolds and nanofibers are being developed for bone tissue regeneration. In this study, we fabricated a hybrid scaffold using a nano-micro precision deposition system. The fabrication process involved the application of the solid freeform fabrication (SFF) technology and electrospinning. The hybrid scaffolds were combined using micro scaffolds and nanofibers. The nanofibers were deposited on each layer of the micro scaffolding using the electrospinning process. The micro scaffolds were fabricated using the SFF technology at a temperature of $100^{\circ}C$, pressure of 650 kPa, and scan velocity of 250 mm/s. Nanofiber fabrication was conducted by means of electrospinning using the flow rate, solution concentration, distance from the tip to the collector (TCD), and voltage. The nanofibers were fabricated using a flow rate of 0.1 ml/min, voltage of 5 kV, TCD of 1 mm, and 10 wt% of solution concentration. MG-63 cells were seeded into the hybrid scaffold for the purpose of its evaluation.

Keywords

Nano-Micro Precision Deposition System;Hybrid Scaffold;Electrospinning;Nanofiber;MG-63 Cell

Acknowledgement

Supported by : 한국연구재단

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