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Evaluation of Various Packaging Systems on the Activity of Antioxidant Enzyme, and Oxidation and Color Stabilities in Sliced Hanwoo (Korean Cattle) Beef Loin during Chill Storage

  • Kang, Sun Moon (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kang, Geunho (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Seong, Pil-Nam (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Park, Beomyoung (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Soohyun (Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2014.02.24
  • Accepted : 2014.06.09
  • Published : 2014.09.01

Abstract

The effects of various packaging systems, vacuum packaging (VACP), medium oxygen-modified atmosphere packaging (50% $O_2/20%$ $CO_2/30%$ $N_2$, MOMAP), MOMAP combined with vacuum skin packaging (VSP-MOMAP), high oxygen-MAP (80% $O_2/20%$ $CO_2/30%$ $N_2$, HOMAP), and HOMAP combined with VSP (VSP-HOMAP), on the activity of antioxidant enzyme, and oxidation and color stabilities in sliced Hanwoo (Korean cattle) beef loin were investigated at $4^{\circ}C$ for 14 d. Higher (p<0.05) superoxide dismutase activity and total reducing ability were maintained in VSP-MOMAP beef than in HOMAP beef. Lipid oxidation (2-thiobarbituric acid reactive substances, TBARS) was significantly (p<0.05) retarded in MOMAP, VSP-MOMAP, and VSP-HOMAP beef compared with HOMAP beef. Production of nonheme iron content was lower (p<0.05) in VSP-MOMAP beef than in HOMAP beef. Red color ($a^*$) was kept higher (p<0.05) in VSP-MOMAP beef compared with MOMAP, HOMAP, and VSP-HOMAP beef. However, VACP beef was found to have the most positive effects on the antioxidant activity, oxidation and red color stabilities among the various packaged beef. These findings suggested that VSP-MOMAP was second to VACP in improving oxidation and color stabilities in sliced beef loin during chill storage.

Keywords

Packaging;Antioxidant;Oxidation Stability;Color;Beef Loin;Hanwoo

Acknowledgement

Supported by : National Institute of Animal Science

References

  1. Sinnhuber, R. O. and T. C. Yu. 1977. The 2-thiobarbituric acid reaction, an objective measure of the oxidative deterioration occurring in fats and oils. J. Jpn. Soc. Fish. Sci. 26:259-267.
  2. Smith, G. C., K. E. Belk, J. N. Sofos, J. D. Tatum, and S. N. Williams. 2000. Economic implications of improved color stability in beef. In: Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality (Eds. E. A. Decker, C. Faustman, and C. J. Lopez-Bote). John Wiley & Sons, Inc, NY, USA. pp. 397-426.
  3. SPSS. 2011. PASW Statistics 21. Statistical Package for the Social Sciences Incorporated, Chicago, IL, USA.
  4. Troy, D. J. and J. P. Kerry 2010. Consumer perception and the role of science in the meat industry. Meat Sci. 86:214-226. https://doi.org/10.1016/j.meatsci.2010.05.009
  5. Vazquez, B. I., L. Carreira, C. Franco, C. Fente, A. Cepeda, and J. B. Velazquez. 2004. Shelf life extension of beef retail cuts subjected to an advanced vacuum skin packaging system. Eur. Food Res. Technol. 218:118-122. https://doi.org/10.1007/s00217-003-0837-6
  6. Xiong, Y. L. and E. A. Decker. 1995. Alterations of muscle protein functionality by oxidative and antioxidative processes. J. Muscle Foods 6:139-160. https://doi.org/10.1111/j.1745-4573.1995.tb00563.x
  7. Xiong, Y. L. 2000. Protein oxidation and implications for muscle food quality. In: Antioxidants on Muscle Foods (Eds. E. Decker, C. Faustman, and C. J. Lopez-Bote). John Wiley and Sons, Inc., NY, USA. pp. 85-111.
  8. Morzel, M., Ph. Gatellier, T. Sayd, M. Renerre, and E. Laville. 2006. Chemical oxidation dcreases proteolytic susceptibility of skeletal muscle myofibrillar proteins. Meat Sci. 73:536-543. https://doi.org/10.1016/j.meatsci.2006.02.005
  9. Muhlisin, Y. R. Song, D. S. Kim, J. H. Lee, J. H. Choi, and S. K. Lee. 2013. Total iron, heme iron and free iron contents in raw and cooked meat of Korean native chicken, duck and Hanwoo. In: Proceedings of 2013 Annual Congress of Korean Society of Animal Sciences and Technology, Jeju, Korea. pp. 229.
  10. O'Grady, M. N., F. J. Monahan, R. M. Burke, and P. Allen. 2000. The effect of oxygen level and exogenous $\alpha$-tocopherol on the oxidative stability of minced beef in modified atmosphere packs. Meat Sci. 55:39-45. https://doi.org/10.1016/S0309-1740(99)00123-0
  11. Reeder, B. J. and M. T. Wilson. 1998. Mechanism of reaction of myoglobin with the lipid hydroperoxide hydroperoxyocta-decadienoic acid. Biochem. J. 330:1317-1323. https://doi.org/10.1042/bj3301317
  12. Renerre, M., F. Dumont, and Ph. Gatellier. 1996. Antioxidant enzyme activities in beef in relation to oxidation of lipid and myoglobin. Meat Sci. 43:111-121.
  13. Reznick, A. Z. and L. Packer. 1994. Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. In: Methods Enzymol. (Ed. L. Packer). Academic Press, Inc., San Diego, CA, USA. pp. 357-363.
  14. Santos, E. M., A. M. Diez, C. Gonzalez-Fernandez, I. Jaime, and J. Rovira. 2005. Microbiological and sensory changes in "Morcilla de Burgos" preserved in air, vacuum and modified atmosphere packaging. Meat Sci. 71:249-255. https://doi.org/10.1016/j.meatsci.2005.03.028
  15. Seyfert, M., R. A. Mancini, M. C. Hunt, J. Tang, and C. Faustman. 2007. Influence of carbon monoxide in package atmospheres containing oxygen on colour, reducing activity, and oxygen consumption of five bovine muscles. Meat Sci. 75:432-442. https://doi.org/10.1016/j.meatsci.2006.08.007
  16. Liu, C., Y. Zhang, X. Yang, R. Liang, Y. Mao, X. Hou, X. Lu, and X. Luo. 2014. Potential mechanisms of maintaining color stability of different bovine muscles. Meat Sci. 97:189-196. https://doi.org/10.1016/j.meatsci.2014.01.027
  17. Lopez-Lorenzo, P., P. Hernandez, B. Sanz-Perez, and J. A. Ordonez. 1980. Effect of oxygen- and carbon dioxide-enriched atmospheres on shelf-life extension of refrigerated ground pork. Meat Sci. 4:89-94. https://doi.org/10.1016/0309-1740(80)90034-0
  18. Lund, M. N., M. S. Hviid, and L. H. Skibsted. 2007a. The combined effect of antioxidants and modified atmosphere packaging on protein and lipid oxidation in beef patties during chill storage. Meat Sci. 76:226-233. https://doi.org/10.1016/j.meatsci.2006.11.003
  19. Lund, M. N., R. Lametsch, M. S. Hviid, O. N. Jensen, and L. H. Skibsted. 2007b. High-oxygen packaging atmosphere influences protein oxidation and tenderness of porcine longissimus dorsi during chill storage. Meat Sci. 77:295-303. https://doi.org/10.1016/j.meatsci.2007.03.016
  20. Marklund, S. L. 1986. Pyrogallol autooxidation. In: CRC Handbook of Methods for Oxygen Radical Research (Ed. R. A. Green). CRC Press, Boca Raton, USA. pp. 243-247.
  21. Mei, L., A. D. Crum, and E. A. Decker. 1994. Development of lipid oxidation and inactivation of antioxidant enzymes in cooked pork and beef. J. Food Lipids 1:273-283. https://doi.org/10.1111/j.1745-4522.1994.tb00252.x
  22. Mercier, Y., P. Gatellier, M. Viau, H. Remignon, and M. Renerre. 1998. Effect of dietary fat and vitamin E on colour stability and on lipid and protein oxidation in turkey meat during storage. Meat Sci. 48:301-318. https://doi.org/10.1016/S0309-1740(97)00113-7
  23. Mercier, Y., P. Gatellier, and M. Renerre. 2004. Lipid and protein oxidation in vitro, and antioxidant potential in meat from Charolais cows finished on pasture or mixed diet. Meat Sci. 66:467-473. https://doi.org/10.1016/S0309-1740(03)00135-9
  24. Min, B and D. U. Ahn. 2005. Mechanism of lipid oxidation in meat and meat products-A review. Food Sci. Biotechnol. 14:152-163.
  25. Harel, S. and J. Kanner. 1985. Muscle membranal lipid peroxidation initiated by $H_2O_2$-activated metmyoglobin. J. Agric. Food Chem. 33:1188-1192. https://doi.org/10.1021/jf00066a042
  26. Jakobsen, M. and G. Bertelsen. 2000. Colour stability and lipid oxidation of fresh beef. Development of a response surface model for predicting the effects of temperature, storage time, and modified atmosphere composition. Meat Sci. 54:49-57. https://doi.org/10.1016/S0309-1740(99)00069-8
  27. Jayasingh, P., D. P. Cornforth, C. E. Carpenter, and D. Whittier. 2001. Evaluation of carbon monoxide treatment in modified atmosphere packaging or vacuum packaging to increase color stability of fresh beef. Meat Sci. 59:317-324. https://doi.org/10.1016/S0309-1740(01)00086-9
  28. Kang, S. M., Muhlisin, G. Y. Kim, S. Cho, B. Park, S. Jung, and S. K. Lee. 2012. Relationship of antioxidant enzyme activity, lipid oxidation, and aroma pattern of Hanwoo (Korean cattle) beef under oxidation-promoted condition. Korean J. Food Sci. Ani. Resour. 32:346-353. https://doi.org/10.5851/kosfa.2012.32.3.346
  29. Lagerstedt, A., M. L. Ahnstrom, and K. Lundstrom. 2011. Vacuum skin pack of beef - A consumer friendly alternative. Meat Sci. 88:391-396. https://doi.org/10.1016/j.meatsci.2011.01.015
  30. Lee, M., R. G. Cassens, and O. R. Fennema. 1981. Effect of meat ions on residual nitrite. J. Food Proc. Preserv. 5:191-205. https://doi.org/10.1111/j.1745-4549.1981.tb00634.x
  31. Lee, S. K., L. Mei, and E. A. Decker. 1996. Lipid oxidation in cooked turkey as affected by added antioxidant enzymes. J. Food Sci. 61:726-728. https://doi.org/10.1111/j.1365-2621.1996.tb12190.x
  32. Li, X., G. Lindahl, G. Zamaratskaia, and K. Lundstrom. 2012. Influence of vacuum skin packaging on color stability of beef longissimus lumborum compared with vacuum and high-oxygen modified atmosphere packaging. Meat Sci. 92:604-609. https://doi.org/10.1016/j.meatsci.2012.06.006
  33. Lindahl, G. 2011. Colour stability of steaks from large beef cuts aged under vacuum or high oxygen modified atmosphere. Meat Sci. 87:428-435. https://doi.org/10.1016/j.meatsci.2010.10.023
  34. Clausen, I., M. Jakobsen, P. Ertbjerg, and N. T. Madsen. 2009. Modified atmosphere packaging affects lipid oxidation, myofibrillar fragmentation index and eating quality of beef. Packag. Technol. Sci. 22:85-96. https://doi.org/10.1002/pts.828
  35. Davis, M. J. and R. T. Dean. 2003. Radical-mediated protein oxidation. Oxford Science Univ., London, England. pp. 215.
  36. Decker, E. A., S. A. Livisay, and S. Zhou. 2000. Mechanisms of endogenous skeletal muscle antioxidants: chemical and physical aspects. In: Antioxidants in Muscle Foods: Nutritional Strategies to Improve Quality. (Ed. E. A. Decker, C. Faustman and C. J. Lopez-Bote). John Wiley & Sons, Inc., NY, USA. pp. 25-60.
  37. Faustman, C., Q. Sun, R. Mancini, and S. P. Suman. 2010. Myoglobin and lipid oxidation interactions: Mechanistic bases and control. Meat Sci. 86:86-94. https://doi.org/10.1016/j.meatsci.2010.04.025
  38. Flohe, L. and W. A. Gunzler. 1984. Assays of glutathione peroxidase. In: Methods Enzymol. (Ed. L. Packer). Academic Press, Inc., London, UK. pp. 114-121.
  39. Franco, D., L. Gonzalez, E. Bispo, A. Latorre, T. Moreno, J. Sineiro, M. Sanchez, and M. J. Nunez. 2012. Effects of calf diet, antioxidants, packaging type and storage time in beef steak storage. Meat Sci. 90:871-880. https://doi.org/10.1016/j.meatsci.2011.10.008
  40. Gheisari, H. R. and H. Motamedi. 2010. Chloride salt type/ionic strength and refrigeration effects on antioxidant enzymes and lipid oxidation in cattle, camel and chicken meat. Meat Sci. 86:377-383. https://doi.org/10.1016/j.meatsci.2010.05.020
  41. Gill, C. O. and J. C. L. Harrison. 1989. The storage life of chilled pork packaged under carbon dioxide. Meat Sci. 26:313-324. https://doi.org/10.1016/0309-1740(89)90015-6
  42. Aebi, H. E. 1983. Catalase. In: Methods of Enzymatic Analysis. (Eds. H. U. Bergmeyer, J. Bergmeyer, and M. Grassl). Verlag Chemie GmbH, Weinheim, Germany. pp. 273-286.
  43. Aviles, C., M. Juarez, I. L. Larsen, A. Rodas-Gonzalez, and J. L. Aalhus. 2014. Effect of multiple vacuum packs on colour development and stability in beef steaks. Meat Sci. 96:124.
  44. Barros-Velazquez, J., L. Carreira, C. Franco, B. I. Vazquez, C. Fente, and A. Cepeda. 2003. Microbiological and physicochemical properties of fresh retail cuts of beef packaged under an advanced vacuum skin system and stored at $4^{\circ}C$. J. Food Prot. 66:2085-2092. https://doi.org/10.4315/0362-028X-66.11.2085
  45. Borch, E., M. L. Kant-Muermans, and Y. Blixt. 1996. Bacterial spoilage of meat and cured meat products. Int. J. Food Microbiol. 33:103-120. https://doi.org/10.1016/0168-1605(96)01135-X
  46. Butterfiled, D. A. and E. R. Stadtman. 1997. Protein oxidation processes in aging brain. Adv. Cell Aging Gerontol. 2:161-191. https://doi.org/10.1016/S1566-3124(08)60057-7
  47. Carter, P. 1971. Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal. Biochem. 40:450-458. https://doi.org/10.1016/0003-2697(71)90405-2
  48. Chan, K. M. and E. A. Decker. 1994. Endogenous skeletal muscle antioxidants. Crit. Rev. Food Sci. Nutr. 34:403-426. https://doi.org/10.1080/10408399409527669
  49. Chen, T., G. H. Zhou, X. L. Xu, G. M. Zhao, and C. B. Li. 2010. Phospholipase A2 and antioxidant enzyme activities in normal and PSE pork. Meat Sci. 84:143-146. https://doi.org/10.1016/j.meatsci.2009.08.039

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