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Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse

  • Bao, Wenlei (College of Life Science, Inner Mongolia University) ;
  • Yin, Jianxin (College of Life Science, Inner Mongolia University) ;
  • Liang, Yan (College of Life Science, Inner Mongolia University) ;
  • Guo, Zhixin (College of Life Science, Inner Mongolia University) ;
  • Wang, Yanfeng (College of Life Science, Inner Mongolia University) ;
  • Liu, Dongjun (College of Life Science, Inner Mongolia University) ;
  • Wang, Xiao (College of Life Science, Inner Mongolia University) ;
  • Wang, Zhigang (College of Life Science, Inner Mongolia University)
  • Received : 2014.01.21
  • Accepted : 2014.03.31
  • Published : 2014.09.01

Abstract

To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant $6{\times}his-gVEGF164$ protein was induced by 0.5 mM isopropyl thio-${\beta}$-D-galactoside at $32^{\circ}C$. Recombinant goat VEGF164 (rgVEGF164) was purified and identified by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.

Keywords

Goat;Vascular Endothelial Growth Factor (VEGF);Recombinant Protein;Hair Growth

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